Journal of Korean Society of Occupational and Environmental Hygiene (한국산업보건학회지)

Korean Industrial Hygiene Association (한국산업보건학회)
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Changes of Pulmonary Function and Biopersistence Evaluation of Sepiolite in Sprague-Dawely Rats

랫드에 주입된 세피오라이트에 의한 폐기능 변화 및 생체내구성 평가

  • Chung, Yong Hyun (Chemical Safety and Health Research Center, Occupational Safety & Health Research Institute) ;
  • Han, Jeong Hee (Chemical Safety and Health Research Center, Occupational Safety & Health Research Institute) ;
  • Sung, Jae Hyuck (Chemical Safety and Health Research Center, Occupational Safety & Health Research Institute) ;
  • Yu, Il Je (Environment & Merchandise Research Institute)
  • 정용현 (산업안전보건연구원화학물질안전보건센터) ;
  • 한정희 (산업안전보건연구원화학물질안전보건센터) ;
  • 성재혁 (산업안전보건연구원화학물질안전보건센터) ;
  • 유일재 (한국생활환경시험연구원)
  • Received : 2006.08.01
  • Accepted : 2006.09.26
  • Published : 2006.12.31
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Abstract

Two kinds of sepiolite (sepiolite 1, sepiolite 2), a $500^{\circ}C$ heat treated sepiolite (sepiolite 500), and a $700^{\circ}C$ heat treated sepiolite (sepiolite 700) were analyzed for their physicochemical properties. After these sepiolites were instilled into rat lungs, the effects of these substances on lung function and biochemical changes were evaluated. In addition, the fibers in the lungs were counted and characterized after the lungs were treated for electron microscopical analysis. The lungs instilled with sepiolites increased their weight and tidal volume statistically significantly compared with the unexposed control. The numbers of lymphocytes and polymorphonuclear cells (PMN) in the bronchoalveolar lavage (BAL) fluid also increased compared with the control, indicating the sepiolite induced inflammation. The heat treated sepiolites, however, did not show any toxicological differences from the untreated sepiolites. Although sepiolite showed less change in fiber atomic % compositions ( sepiolite 500, Si 0.9%, p <0.01 ; sepiolite 700, Si 3.7%, p<0.05) than chrysotile (Si 9.7%, p<0.01), the durability of the fibers in the lungs could not be determined in this subchronic experiment.

Keywords

References

  1. Arts JHE, Bloksma N, Leusink-Muis T and Kuper CF. Respiratory allergy and pulmonary irritation to trimellitic anhydrite in Brown Norway rats. Toxicol Appl Pham 2003; 187: 38-49 https://doi.org/10.1016/S0041-008X(02)00023-6
  2. Bellmann B, Muhle H, Kamstrup O, Graeger UF. Investigation of the biodurability of man made vitreous fibers ion rat lungs. Environ Health perspect 1997; 102(suppl 5): 185-189
  3. Baris YI, Sahin AA and Erkan ML. Clinical and radiological study in sepiolite workers. Archives of Environmental Health 1980; 35(6): 343-346 https://doi.org/10.1080/00039896.1980.10667517
  4. Brack T, Jubran A and Tobin MJ. Dyspnea and decreased variability of breathing in patients with restrictive lung disease. Am J Respir Crit Care Med 2002; 165: 1260-1264 https://doi.org/10.1164/rccm.2201018
  5. Chamberlain M, Davies R, Brown RC and Griffiths DM. In vitro test for the pathogenicity of mineral dusts. Ann Occup Hyg 1982; 26: 583-592 https://doi.org/10.1093/annhyg/26.5.583
  6. Fancine Denizeau, Michel Marine, Gaston Chevalier and Michel G Cote. Absence of genotoxic effects of nonasbestos mineral fibers. Cell Biology and Toxicology 1985; 1: 23-32 https://doi.org/10.1007/BF00717788
  7. Hori H, Kasai T, Haratake J, Ishimatsu S, Oyabu T, Yamato H, Higashi T, Tanaka I. Biological effects of inhaled magnesium sulphate whiskers in rats. Occup Environ Med 1994; 51: 492-9 https://doi.org/10.1136/oem.51.7.492
  8. Ishihara Y. In vitro studies on biological effects on fibrous minerals. Industrial Health 2001; 39: 94-105 https://doi.org/10.2486/indhealth.39.94
  9. Ishihara Y, Kohyama N, Nagai A, Kagawa J. Contribution of human pulmonary macrophage-derived cytokines to asbestos-induced lung inflammation and fibrosis. Inhal Toxicol 1997; 10: 205-25
  10. Ishihara Y, Kohyama N, Nagai A, Kagawa J. Cellular biological effects and a single transtracheal injection test in three types of whisker fibers. Inhal Toxicol 1998; 10: 275-91 https://doi.org/10.1080/089583798197682
  11. Koshi K, Hayashi H and Sakabe H. Cell toxicity and hemolytic action of asbestos dust. Ind Health 1969; 6: 69-79 https://doi.org/10.2486/indhealth.6.69
  12. Mansoor JK, Hyde DM and Schelegle ES. Contribution of vagal afferents to breathing patten in rats with lung fibrosis. Res Physiol 1997; 108: 45-61 https://doi.org/10.1016/S0034-5687(96)02505-4
  13. Morimoto Y and Tanaka I. In vivo studies of man made mineral fibers - fibrosis related factors -. Industrial Health 2001; 39: 106-113 https://doi.org/10.2486/indhealth.39.106
  14. Morimoto Y, Tsuda T, Yamato H, Hori H, Higashi T, Tanaka I. Risk assessment of man made fibers by RT-PCR. In: Advances in the prevention of occupational respiratory disease. ed. Chiyotani K, Hosoda Y, Aizawa Y, Elsevier Science, Amsterdam; 1998. p. 611-4
  15. Morimoto Y, Tsuda T, Hirohashi M, Yamato H, Hori H, Ohgami A, Yatera K, Kim HN, Ding L, Kido M, Higashi T, Tanaka I. Effects of mineral fibers on the gern expression of proinflammantory cytokines and inducible nitric-oxide synthase in alveolar macrophages. Ind Health 1999; 37: 329-34 https://doi.org/10.2486/indhealth.37.329
  16. Porter D, Hubbs AF, Mercer R, Robinson VA, Ramsey D, McLaurin J, Khan A, Battelli L, Brumbaugh K, Teass A and Castranova V. Progression of lung inflammation and damage in rats after cessation of silica inhalation. Toxicological science 2004; 79: 370-380 https://doi.org/10.1093/toxsci/kfh110
  17. Sakabe H, Koshi K and Hayashi H, On the cell toxicity of mineral dusts. Inhaled Particles III. ed. Walton, W. H., Unwin Brothers Limited; 1971. p. 423-434
  18. Sakai K, Hisanaga N, Kojima A, and Takeuchi Y, Elemental composition of asbestos fibers recovered from human lung. Annual Report of Nagoya City Public Health Research Institute 1991; 37: 116-119
  19. Stephen S. Leonard, Kristina Mowrey, Donna Pack, Xianglin Shi, Vince Castranova, Periannan Kuppusamy and Val Vallyathan. In vivo bioassays of acute asbestosis and its correlation with ESR spectroscopy and imaging in redox status, Molecular and Cellular Biochemistry 2002; 234/235: 369-377 https://doi.org/10.1023/A:1015919101174
  20. Tsuda T, Yamato H, Morimoto Y, Oyabu T, Ishimatsu S, Hori H, Kasai T, Kido M, Higashi T, Tanaka I. One year inhalation study of man made fibers, In: Advances in the prevention of occupational respiratory disease. eds, by Chiyotani K, Hosoda Y, Aizawa Y Elsevier Science. Amsterdam; 1998. p. 596-600
  21. Vilcek J, Lee TH. Tumer necrosis factor: New insights into the moleucular mechanisms of its multiple actions. J Biol Chem 1991; 266: 7313-6
  22. Wright A, Gormley IP, Collings PL and Davis MG. The cytotoxicities of asbestos and other fibrous dusts, In : In vitro effects of mineral dusts (Edited by Brown R. C., et al.) Academic Press; 1980. p 25-31
  23. Yamato H, Tanaka I, Higashi T, Kido M. Determinant factor for clearance of ceramic fibers from rat lungs. Br J Ind Med 1992; 49: 182-5
  24. Yamato H, Tanaka I, Higashi T, Kido M. Clearance of inhaled ceramic fibers from rat lungs. Environ Health Perspect 1994; 102(suppl 5): 169-71 https://doi.org/10.1289/ehp.94102s5169
  25. Zhang Y, Lee TC, Guillemin B, Yu M-C, Rom WN. Enhance ILbeta and tumor necrosis fator- realese and messenger RNA expression in macrophages from isiopathic pulmonary fibrosis or after asbestos exposure. J Immunol 1993; 150: 4188-96