Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

The Chemokine SDF-1α Suppresses Fibronectin-mediated In Vitro Lymphocytes Adhesion

  • Ji, LiLi (Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine) ;
  • Sheng, YuChen (The Department of Pharmacology, Shanghai Institute of Pharmaceutical Industry) ;
  • Wang, ZhengTao (Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine)
  • Received : 2006.08.18
  • Accepted : 2006.11.07
  • Published : 2006.12.31
⟨ Previous Next ⟩

Abstract

Stromal cell-derived factor (SDF-1) is a CXC chemokine that selectively activates the CXCR4 chemokine receptor. Fibronectin is an intracellular matrix component that binds integrin and mediates cell-matrix adhesion. Activation of the integrin receptor can occur in two ways: by ligand binding (outside-in signaling), and in response to intracellular events (inside-out signaling). In the current study we showed that SDF-$1{\alpha}$ inhibited adhesion of T lymphocyte Jurkat cells resulting from binding high concentrations of fibronectin as well as that of THP-1 monocytes. The effect of SDF-$1{\alpha}$ on fibronectin-mediated adhesion was partly reversed by the CXCR4 receptor antagonist T140. Our results suggest that an SDF-1/CXCR4 signal pathway modulates fibronectin-mediated lymphocytes adhesion.

Keywords

References

  1. Campbell, J., Qin, S., Bacon, K. B., Mackay, C. R., and Butcher, E. C. (1996) Biology of chemokine and classical chemoattractant receptors: differential requirements for adhesiontriggering versus chemotactic responses in lymphoid cells. J. Cell Biol. 134, 255−266
  2. Chen, L., Pei, G., and Zhang, W. B. (2004) An overall picture of chemokine receptors: Basic research and drug development. Curr. Pharm. Design 10, 1045−1055 https://doi.org/10.2174/1381612043452749
  3. Clark, E. A. and Brugge, J. S. (1995) Integrins and signal transduction pathways: the road taken. Scinece (Wash. DC) 268, 233−239
  4. Danen, E. H., Aota, S., Van Kraats, A. A., Yamada, K. M., Ruiter, D. J., et al. (1995) Requirement for the synergy site fro cell adhesion to fibronectin depends on the activation state of integrin alpha5 beta1. J. Biol. Chem. 270, 21612−21618
  5. Frade, J. M. R., Liorente, M., Mellado, M., Alcami, J., Gutierrez- Ramos, J. C., et al. (1997) The amino-terminal domain of the CCR2 chemokine receptor acts as coreceptor for HIV-1 infection. J. Clin. Invest. 100, 497−502 https://doi.org/10.1172/JCI119558
  6. Garcia-Bernal, D., Wright, N., Sotillo-Mallo, E., Nombela- Arrieta, C., Stein, J. V., et al. (2005) Vav1 and Rac control chemokine-promoted T lymphocyte adhesion mediated by the integrin alpha4beta1. Mol. Biol. Cell 16, 3223−3235 https://doi.org/10.1091/mbc.E04-12-1049
  7. Gleichmann, M., Gillen, C., Czardybon, M., Bosse, F., Greiner- Petter, R., et al. (2000) Cloning and characterization of SDF- 1$\gamma$, a novel chemokine transcript with developmentally regulated expression in the nervous system. Eur. J. Neurosci. 12, 1857−1866 https://doi.org/10.1046/j.1460-9568.2000.00048.x
  8. Gotoh, A., Reid, S., Miyazawa, K., and Broxmeyer, H. E. (1999) SDF-1 suppresses cytokine-induced adhesion of human haemopoietic progenitor cells to immobilized fibronectin. Br. J. Haematol. 106, 171−174 https://doi.org/10.1046/j.1365-2141.1999.01517.x
  9. Grabovsky, V., Feigelson, S., Chen, C., Bleijs, D. A., Peled, A., et al. (2000) Subsecond induction of alpha4 integrin clustering by immobilized chemokines stimulates leukocytes tethering and rolling in endothelial vascular cell adhesion molecules 1 under flow conditions. J. Exp. Med. 192,495−506
  10. Hedrick, J. A. and Zlotnik, A. (1996) Chemokines and lymphocyte biology. Curr. Opin. Immunol. 8, 343−347
  11. Hernandez-Lopez, C., Varas, A., Sacedon, R., Jimenez, E., Munoz, J. J., et al. (2002) Stromal cell-derived factor 1/CXCR4 signaling is critical for early human T-cell development. Blood 99, 546−554 https://doi.org/10.1182/blood.V99.2.546
  12. Horuk, R. (2001) Chemokine receptors. Cytokine Growth Factor Rev. 12, 313−335
  13. Hynes, R. O. (1987) Integrins: a family of cell surface receptors. Cell 48, 549−554
  14. Kijowski, J., Baj-Krzyworzeka, M., Majka, M., Reca, R., Marquez, L. A., et al. (2001) The SDF-1-CXCR4 axis stimulates VEGF secretion and activates integrins but does not affect proliferation and survival in lymphohematopoietic cells. Stem Cells 19, 453−466 https://doi.org/10.1634/stemcells.19-5-453
  15. Laakko, T. and Juliano, R. L. (2003) Adhesion regulation of stromal cell-derived factor-1 activation of ERK in lymphocytes by phosphatases. J. Biol. Chem. 278, 31621−31628 https://doi.org/10.1074/jbc.M304700200
  16. Lauwaet, T., Oliveira, M. J., Mareel, M., and Leroy, A. (2000) Molecular mechanisms of invasion by cancer cells, leukocytes and microorganisms. Microbes Infect. 2, 923−931
  17. Libura, J., Drukala, J., Majka, M., Tomescu, O., Navenot, J. M., et al. (2002) CXCR-SDF-1 signaling is active in rhabdomyosarcoma cells and regulates locomotion, chemotaxis, and adhesion. Blood 100, 2597−2606 https://doi.org/10.1182/blood-2002-01-0031
  18. Liu, L., Schwartz, B. R., Tupper, J., Lin, N., Winn, R. K., et al. (2002) The GTPase Rap1 regulates phorbol 12-myristate 13- acetate-stimulated but not ligand-induced $\beta_{1}$ integrin-dependent leukocyte adhesion. J. Biol. Chem. 277, 40893−40900 https://doi.org/10.1074/jbc.M206208200
  19. Luster, A. D. (2002) The role of chemokines in linking innate and adaptive immunity. Curr. Opin. Immunol. 14, 129−135
  20. Ma, Q., Jones, P., Borghesani, R., Segal, R. A., Nagasawa, T., et al. (1998) Impaired B-lymphopoiesis, myelopoiesis, and derailed cerebellar neuron migration in CXCR4- and SDF-1- deficient mice. Proc. Natl. Acad. Sci. USA 95, 9448−9453
  21. Nagasawa, T., Kikutani, H., and Kishimoto, T. (1994) Molecular cloning and structure of a pre-B-cell growth stimulating factor. Proc. Natl. Acad. Sci. USA 91, 2305−2309
  22. Nagasawa, T., Tachibana, K., and Kishimoto, T. (1998) A novel CXC chemokine PBSF/SDF-1 and its receptor CXCR4: their function in development, hematopoiesis and HIV infection. Semin. Immunol. 10, 179−185 https://doi.org/10.1006/smim.1998.0128
  23. Odemis, V., Moepps, B., Gierschik, P., and Engele, J. (2002) Interleukin-6 and cAMP induce SDF-1 chemotaxis in astroglia by upregulating CXCR4 cell surface expression: implication for brain inflammation. J. Biol. Chem. 277, 39801−39808 https://doi.org/10.1074/jbc.M200472200
  24. Parkes, R. J. and Hart, S. L. (2000) Adhesion molecules and gene transfer. Adv. Drug Deliv. Rev. 44, 135−152
  25. Pelletier, A. J., Van Der Laan, L. J. W., Hildbrand, P. H., Siani, M. A., Thompson, D. A., et al. (2000) Presentation of chemokine SDF-1$\alpha$ by fibronectin mediates directed migration of T cells. Blood 96, 2682−2690
  26. Nanki, T. and Lipsky, P. E. (2000) Cutting edge: stromal cellderived factor-1 is a costimulator for CD4+ T cell activation. J. Immunol. 164, 4121−4126
  27. Shirozu, M., Nakano, T., Inazawa, J., Tashiro, K., Tada, H., et al. (1995) Structural and chromosomal localization of the human stromal cell-derived factor1 (SDF-1) gene. Genomics 28, 495−500 https://doi.org/10.1006/geno.1995.1180
  28. Stantchev, T. S. and Broder, C. C. (2001) Human immunodeficiency virus type-1 and chemokines: beyond competition for common cellular receptors. Cytokine Growth Factor Rev. 12, 219−243 https://doi.org/10.1016/S1359-6101(00)00033-2
  29. Suzuki, K., Saito, J., Yanai, R., Yamada, N., Chikama, T., et al. (2003) Cell-matrix and cell-cell interactions during corneal epithelial wound healing. Prog. Retin. Eye Res. 22, 113−133 https://doi.org/10.1016/S1350-9462(02)00042-3
  30. Tachibana, K., Hirota, S., Lizasa, H., Yoshida, H., Kawabata, K., et al. (1998) The chemokine receptor CXCR4 is essential for vascularization of the gastrointestinal tract. Nature 393, 591−595
  31. Takami, M., Terry, V., and Petruzzelli, L. (2002) Signaling pathways involved in IL-8-dependent activation of adhesion through Mac-1. J. Immunol. 168, 4559−4566
  32. Tamamura, H., Fujisawa, M., Hiramatsu, K., Mizumoto, M., Nakashima, H., et al. (2004) Identification of a CXCR4 antagonist, a T140 analog, as an anti-rheumatoid arthritis agent. FEBS Lett. 569, 99−104 https://doi.org/10.1016/j.febslet.2004.05.056
  33. Weber, K. S. C., Klickstein, L. B., and Weber, C. (1999) Specific ativation of leukocytes $\beta2$ integrins lymphocyte function- associated antigen-1 and Mac-1 by chemokines mediated by distinct pathways via the $\alpha$ subunit cytoplasmic domains. Mol. Biol. Cell 10, 861−873
  34. Wong, M. and Fish E. N. (1998) RANTES and MIP-1$\alpha$ activates stats in T cells. J. Biol. Chem. 273, 309−314 https://doi.org/10.1074/jbc.273.1.309
  35. Zhao, J., Ben, L. H., Wu, Y. L., Hu, W., and Ling, K., et al. (1999) Anti-HIV agent Trichosanthin enhances the capabilities of chemokines to stimulate chemotaxis and G protein activation, and this is mediated through interaction of Trichosanthin and chemokine receptors. J. Exp. Med. 190, 101−111 https://doi.org/10.1084/jem.190.1.101
  36. Zlotnik, A., Morales, J., and Hedrick, J. A. (1999) Recent advances in chemokines and chemokine receptors. Crit. Rev. Immunol. 19, 1−47
  37. Zou, Y. R., Kottmann, A. H., Kuroda, M., Taniuchi, I., and Littman, D. R. (1998) Function of the chemokine receptor CXCR4 in haematopoiesis and in cerebellar development. Nature 393, 595−599