Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Enhanced Heterologous Expression of Aspergillus niger Epoxide Hydrolase and Its Application to Enantioselective Hydrolysis of Racemic Epoxides

Aspergillus niger의 Epoxide Hydrolase 고효율 발현 및 라세믹 에폭사이드의 입체선택적 가수분해

  • Lee, Soo Jung (Department of Food Science and Technology, College of Engineering, Kyungsung University) ;
  • Kim, Hee Sook (Department of Food Science and Technology, College of Engineering, Kyungsung University) ;
  • Lee, Eun Yeol (Department of Food Science and Technology, College of Engineering, Kyungsung University)
  • 이수정 (경성대학교 공과대학 식품공학과) ;
  • 김희숙 (경성대학교 공과대학 식품공학과) ;
  • 이은열 (경성대학교 공과대학 식품공학과)
  • Received : 2005.08.25
  • Accepted : 2006.06.12
  • Published : 2006.10.10
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Abstract

The epoxide hydrolase (EH) of Aspergillus niger LK was expressed to high levels in Escherichia coli based on codon usage. E. coli, Rosetta (DE3)PLysS, containing a large number of tRNAs for rare-codons was employed as a host strain. The recombinant E. coli expressing A. niger EH showed an enhanced enantioselective hydrolysis activity toward racemic styrene oxide. Enantiopure (S)-styrene oxide with a high enantiopurity of 99% ee was obtained from racemic substrates.

Aspergillus niger LK의 epoxide hydrolase (EH)를 codon usage를 고려한 Escherichia coli 균주에서 고효율로 발현할 수 있었다. E. coli에서 잘 사용되지 않는 rare codon에 대한 tRNA 유전자 정보가 들어있는 plasmid를 함유한 E. coli 균주인 Rosetta (DE3)PLysS를 숙주세포로 사용하였다. A. niger EH를 발현시킨 재조합 E. coli를 생촉매로 사용하여 라세믹 styrene oxide 혼합물과 반응시켰을 때, (R)-styrene oxide에 대한 입체선택적 가수분해활성이 향상됨을 확인할 수 있었다. 또한 라세믹 기질로부터 입체적으로 고순도인 99% ee 값을 갖는 광학적으로 순수한 (S)-styrene oxide를 얻을 수 있었다.

Keywords

References

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