Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Identification of Medicinal Mushroom Species Based on Nuclear Large Subunit rDNA Sequences

  • Lee Ji Seon (Institute of Biotechnology, Yeungnam University) ;
  • Lim Mi Ok (College of Pharmacy, Yeungnam University) ;
  • Cho Kyoung Yeh (College of Pharmacy, Yeungnam University) ;
  • Cho Jung Hee (Division of Natural drug Evaluation, Korea Food and Drug Administration) ;
  • Chang Seung Yeup (Division of Natural drug Evaluation, Korea Food and Drug Administration) ;
  • Nam Doo Hyun (College of Pharmacy, Yeungnam University)
  • Published : 2006.02.01
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Abstract

The purpose of this study was to develop molecular identification method for medical mushrooms and their preparations based on the nucleotide sequences of nuclear large subunit (LSD) rDNA. Four specimens were collected of each of the three representative medicinal mushrooms used in Korea: Ganoderma Incidum, Coriolus versicolor, and Fomes fomentarius. Fungal material used in these experiments included two different mycelial cultures and two different fruiting bodies from wild or cultivated mushrooms. The genomic DNA of mushrooms were extracted and 3 nuclear LSU rDNA fragments were amplified: set 1 for the 1.1-kb DNA fragment in the upstream region, set 2 for the 1.2-kb fragment in the middle, and set 3 for the 1.3-kb fragment downstream. The amplified gene products of nuclear large subunit rDNA from 3 different mushrooms were cloned into E. coli vector and subjected to nucleotide sequence determination. The sequence thus determined revealed that the gene sequences of the same medicinal mushroom species were more than $99.48\%$ homologous, and the consensus sequences of 3 different medicinal mushrooms were more than $97.80\%$ homologous. Restriction analysis revealed no useful restriction sites for 6-bp recognition enzymes for distinguishing the 3 sequences from one another, but some distinctive restriction patterns were recognized by the 4-bp recognition enzymes AccII and HhaI. This analysis was also confirmed by PCR-RFLP experiments on medicinal mushrooms.

Keywords

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