Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antibody Layer Fabrication for Protein Chip to Detect E. coli O157:H7, Using Microcontact Printing Technique

  • KIM HUN-SOO (Department of Chemical and Biomolecular Engineering, and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Technology Planning Team, Corporate R&D Center, Samsung SDI Co. Ltd,) ;
  • BAE YOUNG-MIN (Department of Chemical and Biomolecular Engineering, and Interdisciplinary Program of Integrated Biotechnology, Sogang University) ;
  • KIM YOUNG-KEE (Department of Chemical Engineering, Hankyong National University) ;
  • OH BYUNG-KEUN (Department of Chemical and Biomolecular Engineering, and Interdisciplinary Program of Integrated Biotechnology, Sogang University) ;
  • CHOI JEONG-WOO (Department of Chemical and Biomolecular Engineering, and Interdisciplinary Program of Integrated Biotechnology, Sogang University)
  • Published : 2006.01.01
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Abstract

An antibody layer was fabricated to detect Escherichia coli O157:H7. The micropattern of 16-mercaptohexadecanoic acid (16-MHDA) as alkylthiolate was formed on the gold surface by using the PDMS stamp with microcontact printing $({\mu}CP)$ techniques. In order to form antibody patterns on the template, protein G was chemically bound to the 16-MHDA patterns, and antibody was adsorbed on a self-assembled protein G layer. The formation of the 16-MHDA micropattern, self-assembled protein G layer and antibody pattern on Au substrate was confirmed by surface plasmon resonance (SPR) spectroscopy. Finally, the micropatterning method was applied to fabricate the antibody probe for detection of E. coli O157:H7, and monitoring of antigen by using this probe was successfully achieved.

Keywords

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