Nutritional Sciences

The Korean Nutrition Society (한국영양학회)
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Inhibitory Effects of (-) Epigallocatechin Gallate and Quercetin on High Glucose-induced Endothelial Cytotoxicity

  • Choi Yean Jung (Department of Food and Nutrition and Institute of Korea Nutrition Research, Hallym University) ;
  • Kwon Hyang Mi (Department of Food and Nutrition and Institute of Korea Nutrition Research, Hallym University) ;
  • Choi Jung Suk (Department of Food and Nutrition and Institute of Korea Nutrition Research, Hallym University) ;
  • Bae Ji Young (Department of Food and Nutrition and Institute of Korea Nutrition Research, Hallym University) ;
  • Kang Sang Wook (Department of Food and Nutrition and Institute of Korea Nutrition Research, Hallym University) ;
  • Lee Sang Soo (Department of Orthopaedic Surgery, Hallym University) ;
  • Lee Yong Jin (Chuncheon Bioindustry Foundation) ;
  • Kang Young Hee (Department of Food and Nutrition and Institute of Korea Nutrition Research, Hallym University)
  • Published : 2006.02.01
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Abstract

Functional damage to microvascular endothelial cells by hyperglycemia is thought to be one of the critical risk factor.; in the impaired wound healing seen with diabetes mellitus. It is also thought that oxidative stress plays a significant role in this endothelial cell dysfunction. The present study examined the differential effects of flavonoids on endothelial cell dysfunction under high glucose conditions. Human endothelial cells exposed to 30 mmol/L glucose for 7 d were pre-treated with various flavonoids and pulse-treated with 0.2 mmol/L $H_2O_2$ for 30 min. High glucose markedly decreased cell viability with elevated oxidant generation and nuclear condensation. $H_2O_2$ insult exacerbated endothelial cytotoxicity due to chronic exposure to high glucose. (-)Epigallocatechin gallate and quercetin improved glucose-induced cell damage with the disappearnnce of apoptotic bodies, whereas apigenin intensified the glucose cytotoxicity. In addition, cell viability data revealed that these flavonoids of (-)epigallocatechin gallate and quercetin substantially attenuated both high glucose- and $H_2O_2$- induced dual endothelial damage. These results suggest that (-)epigallocatechin gallate and quercetin may be beneficial agents for improving endothelial cell dysfunction induced by high glucose and may prevent or reduce the development of diabetic vascular complications.

Keywords

References

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