Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Inhibition of Oxidative Damage by Phlorotannins from Ecklonia cava in Normal Human Dermal Fibroblasts

  • Kim, Moon-Moo (Marine Bioprocess Research Center, Pukyong National University) ;
  • Rajapakseb, Niranjan (Department of Chemistry, Pukyong National University) ;
  • Kim, Se-Kwon (Marine Bioprocess Research Center, Pukyong National University)
  • Published : 2006.06.30
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Abstract

Phlorotannins which is oligomeric polyphenol of phloroglucinol unit were isolated from solvent fractions of methanolic extract of the brown alga, Ecklonia cava (EC). The inhibitory effects of phlorotannins from EC solvent fractions on oxidative stress were examined in human dermal fibroblasts (HDFs) related to wrinkle formation. Among the solvent fractions, phlorotannins from ethyl acetate fraction exerted the highest scavenging effect on DPPH radical, hydroxyl radical and alkyl radical analyzed by electron spin resonance (ESR) spectroscopy. The levels of intracellular reactive oxygen species (ROS) and lipid peroxidation were measured using 2',7'-dichlorofluorescin diacetate (DCFH-DA) and diphenyl-1-pyrenylphosphine (DPPP), respectively. Their levels were significantly decreased in the presence of phlorotannins from ethyl acetate fraction, compared with other fractions obtained from EC extract (P < 0.01). Furthermore, intracellular glutathione (GSH) level was significantly increased in a time dependent manner by the phlorotannins. Therefore, these results suggest that phlorotannins from EC extract could have a therapeutic potential for prevention and treatment of several diseases such as wrinkle formation related to oxidative stress.

Phloroglucinol 단위체로 구성된 Oligomeric polyphenol인 Phlorotannins을 갈조류의 일종인 감태(Ecklonia cava)의 메탄올 추출물의 용매 분획으로부터 분리하였다. 산화스트레스에 대한 감태추출물의 용매분획으로부터 Phlorotannins의 억제효능을 주름형성과 연관성이 있는 사람피부섬유아세포(HDFs)에서 조사되었다. ESR spectroscopy 분석에서, 감태의 에틸아세테이트 분획으로 부터의 Phlorotannins에서 DPPH radical, Hydroxyl radical 및 Alkyl radical에 대하여 가장 높은 소거효능이 나타났다. 2',7'-Dichlorofluorescin diacetate (DCFH-DA)와 Diphenyl-1-pyrenylphosphine (DPPP)을 이용하여 세포내에서 활성산소종 및 지질과산화 수준을 측정하였다. 감태의 다른 용매 분획과 비교하여 에틸아세테이트 용매분획으로 부터의 Pphlorotannins의 존재에서 이들수준이 유의성 있게 감소되었다 (P < 0.01). 더욱이, Phlorotannins은 세포내의 Glutathione (GSH) 함량도 시간에 따라 증가시켰다. 그러므로, 이러한 결과들은 감태의 Phlorotannins이 산화적 스트레스와 연관성이 있는 주름형성 같은 여러가지 질환의 예방 및 치료에 잠재적인 효능이 있다는 것을 암시하고 있다.

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