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Effects of Slow Release Recombinant Porcine Somatotropin (rpST) Administration on Growth Performance and pST and IGF-1 of Blood in Finishing Pigs

지속형 유전자 재조합 pST(Recombinant Porcine Somatotropin; rpST) 투여가 비육돈의 성장, 혈중 pST 및 IGF-1 농도에 미치는 영향

  • Published : 2006.10.31

Abstract

The present study was performed to investigate the effect of plant type recombinant porcine somatotropin (pST) adminstration on growth performance and blood profile of finishing pigs. Forty-eight Landrace barrows weighing 78 kg were employed for six week growth trial. Twelve barrows were allocated into various rpST types ; the control (CONT), the 4:1 type rpST (TRT 1), the 1:1 type rpST (TRT 2); and the 4:1 type rpST group(TRT 3) respectively. CONT group were not planted rpST. TRT 1 group was treated with rpST once a week for 6 weeks. Each dose contained the rpST equivalent to 100mg from initial to two weeks and 125mg from three weeks to finial week. TRT 2 and TRT 3 groups were planted rpST four times contained the rpST equivalent to 100mg during trial period from initial to two weeks, and 125 mg from three weeks, to four weeks, respectively. All pigs were fed a commercial feed containing 0.9% lysine ad lib. Daily gain increased by 19.4% in TRT 1(p<0.05) compared to the CONT. rpST improved feed/gain by 13.4~28.9% in all treatment groups(p<0.05). Back fat thickness of the all rpST treated groups were 23.3~29.2% thinner than that of the CONT. These results indicated that the rpST stimulated growth performance in finishing pigs and plant type rpST could be used as a growth stimulant for finishing pigs.

국내에서 제조된 서방출형 유전자재조합 돼지 성장호르몬 (porcine somatotropin; pST)이 돼지의 성장과 등지방두께에 미치는 영향을 구명하기 위해 평균체중이 78kg인 랜드레이스 거세돈 48두를 공시하여 대조구, 4:1제형 rpST구 (6회 투여), 1:1제형 rpST구 (4회 투여), 4:1제형 rpST구 (4회 투여)에 각각 12두씩 배치하였다. 대조구는 pST를 투여하지 않았고, rpST 처리구는 1주일에 1회, 목 근육에 서방형 제재를 주입하였는데, 6회 투여구는 개시부터 2주까지는 100mg을, 3주부터 6주까지는 125mg을, 4회 투여구는 개시부터 2주까지는 100mg을, 3주부터 4주까지는 125mg을 투여하였다. 시험사료는 라이신 함량이 0.9%인 시판 육성돈사료를 무제한 급여한 결과 (주) LG 화학에서 제조한 서방출형 유전자재조합 pST는 증체량 및 사료요구율을 개선시켜 생산성 향상과 등지방두께 감소로 육질이 향상되었다.

Keywords

References

  1. Alan, L. R. 1990. Safety and efficacy of sustained-release recombinantly derived bovine somatotropin administered to lactating dairy cows. Graduate Thesis Minnesota Univ
  2. Boyd, R. D. and Bauman, D. E. 1988. Mechanisms of action for somatotropin in growth. In : Campion, D. R., Hausman, G. J. and artin, R, J. (Eds.) Animal growth regulation. Plenum Press. pp. 257-284
  3. Boyd, R. D., Bauman, D. E., Beerman, D. H., DeNeergard, A. F., Souza, L. and Butler, W. R. 1986. Titration of the porcine growth hormone dose which maximizes growth performance and lean deposition in swine. J. Anim. Sci. 63 (Suppl. 1):218
  4. Brameld, J. M., Atkinson, J. L., Saunders, J. C, Pell, J. M., Buttery, P. J. and Gilmour, R. S. 1996. Effects of growth hormone administration and ditary protein intake on insulin-like growth factor I and growth hormone receptor mRNA expression in porcine liver, skeletal muscle, and adipose tissue. J. Anim. Sci. 74:1832-1841
  5. Campbell, R. G., Steele, N. C., Capema, T. J., McMurtry, J. P., Solomon, M., Solomon, B. and Mitchell, A D. 1989. Interrelationships between sex and exogenous growth hormone administration on performance body composition and protein and fat accretion of growing pigs. J. Anim. Sci. 67:177-186 https://doi.org/10.2527/jas1989.671177x
  6. Chilliard, Y. 1990. Long-term effects of recombinant bovine somatotropin (rbST). In use of Somatotropin in Livestock Production(Sejrsen K., Vestergaard, M. and Niemann-Sorensen, A, aditors). Elsevier Applied Science New York
  7. Chung, C. S., Meserole, V. K. and Etherton, T. D. 1983. Temporal nature of insulin binding and insulin-stimulated glucose metabolism in isolated swine adippoytes. J. Anim. Sci. 56:58-63 https://doi.org/10.2527/jas1983.56158x
  8. Clemmons, D. L., Klibanski, A., Underwood, L. E., McArthur, J. W., Ridgway, E. C, Beithins, I. Z. and Van Wyk, J. J. 1981. Reduction of plasma immunoreactive somatomedic-C. J. Clin. Endocrinol. Metab. 53:1247-1250 https://doi.org/10.1210/jcem-53-6-1247
  9. Dunshea, F. R., Bauman, D. E., Boyd, R. D. and Bell, A. W. 1992. Temporal response of circulating metabolites and hormones during somatotropin treatment of growing pigs. J. Anim. Sci. 70: 123-131 https://doi.org/10.2527/1992.701123x
  10. Etherton, T. D. and Chung, C. S. 1981. Preparation, characterization, and insulin sensitivity of isolated swine adipocytes : Comparison with adipose tissueslices. J. Lipid Res. 22:1053-1059
  11. Etherton, T. D., Wiggins, J. P., Evock, C. M., Chung, C. S., Rebhun, J. F., Walton, P. E. and Steele, N. C. 1987. Stimulation of pig growth performance by porcine growth hormone Determination of the does-response relationship. J. Anim. Sci. 63:433-443
  12. Evans, H. M. and Long, J. A. 1922. Characteristic effects upon growth, oestrus and ovulation induced by the intraperitoneal administration of fresh anterior hypophyseal substance. Proc. Natl. Acad. Sci. USA. 8:38-39
  13. Evock, C. M., Etherton, T. D., Chung, C. S. and Ivy, R. E. 1988. Pituitary porcine growth hormone (pGH) and a recombinant pGH analog stimulate pig growth performance in a similar manner. J. Anim. Sci. 66:1928-1941 https://doi.org/10.2527/jas1988.6681928x
  14. Garssen, G. S. and Oldenbroek, J. K. 1992. Somatotropins in animal production. In : N. A. A. Macfarlane(Ed.) Biotechnological Innovations in Animal productivity. Butterworth-Heinemann Ltd., Oxford
  15. Goodband, R. D., Nelssen, J. L., Hines, R. H., Kropf, D. H., Thaler, R. C, Schricker, B. R., Fitzner, G. E. and Lewis, A. J. 1990. The effects of porcine somatotropin and dietary lysine on growth performance and carcass characteristics of finishing swine. J. Anim. Sci. 68:3261-3276 https://doi.org/10.2527/1990.68103261x
  16. Hacker, R. R., Deschutter, A., Adeola, O. and Kasser, T. R. 1993. Evaluation of long-term somatotropin implants in finishing pigs. J. Anim. Sci. 71:564-570
  17. Hadlay, M. E. 1988. Endocrinology(2nd ed.). Prentile-Hall Intrl. INC., New Jersey
  18. Joo, S. T., Kauffman R. G., Kim, B. C. and Kim, C. J. 1995. The relationship between color and water-holding capacity in postrigor porcine longissimus muscle. J. Muscle Foods. 6:211-226 https://doi.org/10.1111/j.1745-4573.1995.tb00568.x
  19. Joo, S. T., Kauffman, R. G., Kim, B. C. and Park, G. B. 1999. The relationship of sarcoplasmic myofibrillar protein solubility to colour and water-holding capacity in porcine longissius muscle. Meat Sci. 52:291-297 https://doi.org/10.1016/S0309-1740(99)00005-4
  20. Knight, C. D., Kasser, T. R., Swenson, G. H., Hintz, R. L., Azain, M. J., Bates, R. O., Cline, T. R., Crenshaw, J. D., Cromwell, G. L., Hedrick, H. B., Jones, S. T., Kropf, D. H., Lewis, A. J., Mahan, D. C, Mckeith, F. M., McLaughlin, C. L., Nelssen, J. L., Novakofski, J. E., Orcutt, M. W. and Parrett, N. A. 1991. The performance and carcass composition response of finishing swine to a range of porcine somatotropin doses in a l-week delivery system. J. Anim, Sci. 69:4678-4689 https://doi.org/10.2527/1991.69124678x
  21. Meisinger, D. J. 1989. Question and dnswers about porcine somatotropin. Pitman-Moore, Persornal Communication
  22. SAS. 1995. SAA/STAT User's Guide : Version 6, 11th edition. SAS Institute Inc., Cary, NC
  23. Smith, V. G. and Kasson, C. W. 1991. The interrelationship between crude protein and exogenous porcine somatotropin on growth, feed and carcass measurements of pigs. J. Anim. Sci. 69: 571-577 https://doi.org/10.2527/1991.692571x
  24. Wolverton, C. K., Azain, M. J., Duffy, J. Y., White, M. E. and Ramsay, T. G. 1992. Influence of somatotropin on lipid metabolism and IGF gene expression in porcine adipose tissue. Am. J. Physiol. Endocrinol. Metab. 263:E637-E645