The Influence of Hydrogen Peroxide Treatment on Water Stress, Photosynthesis and Thermotolerance of Cucumber(Cucumis sativus) in Greenhouse Cultivation during Summer

Hydrogen Peroxide 처리가 여름철 시설오이의 수분 스트레스, 광합성, 내서성에 미치는 영향

  • Published : 2006.03.01

Abstract

This studies were carried out in summer season to increase high temperature tolerance using hydrogen peroxide treatments on cucumber in greenhouse. The water stress of cucumber in greenhouse by the hydrogen peroxide treatments showed as control>250 mM>500 mM treatments in order. The photosynthesis rate of cucumber at $30^{\circ}C$ did not show difference with each hydrogen peroxide treatment in temperature controlled greenhouse. However, the photosynthesis rate of cucumber in the control and hydrogen peroxide treatments at $40^{\circ}C$ was significantly different. The photosynthesis rate of cucumber in combined treatment with 1,000 $mg{\cdot}L^{-1}\;CO_2$ supply and hydrogen peroxide was also higher than control, however, there was no different of photosynthesis in 250 mM and 500 mM treatment. The value of $F_v/F_m$ and $F_m/F_o$ of chlorophyll fluorescent in 500 mM hydrogen peroxide treatment at $40^{\circ}C$ was highest. Also the activity of POD, the antioxidant enzyme, was higher with high hydrogen peroxide concentration than the other treatments. The high temperature limits for growth were $43^{\circ}C$ in the control, $44^{\circ}C$ in the 250 mM and $46^{\circ}C$ in the 500 mM according to analyze chlorophyll fluorescent $F_o$. The high temperature tolerance in cucumber increased approximately $3^{\circ}C$ by the hydrogen peroxide treatments under this experiment conditions.

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References

  1. Chamnongpol S., H. Willekens, W. Moeder, C. Langebartels, H. Jr. Sandermann, and M. van Montagu. 1998. Defens activation and enhanced pathogen tolerance induced by $H_{2}O_{2}$ in transgenic plants. Proc. Natl. Acad. Sci. USA 95:5818-5823
  2. Oat J., S. Vandenabeele, E. Vranova, M. Van Montagu, D. Inze, and F. Van Breusegem. 2000. Dual action of the active oxygen species during plant stress responses. Cell. Mol. Life Sci. 57:779-795 https://doi.org/10.1007/s000180050041
  3. Idso, S.B. 1982. Non-water-stressed baselines: a key to measuring and interpreting plant water stress. Agricultural Meteorology 27:59-70 https://doi.org/10.1016/0002-1571(82)90020-6
  4. Jackson, R.D., S.B. Idso, R.J. Reginato, and P.J. Pinter Jr. 1981. Canopy temperature as a crop water stress indicator. Water Resources Research 17:11-33 https://doi.org/10.1029/WR017i001p00011
  5. Lu, H. and V.J. Higgins. 1999. The effect of hydrogen peroxide on the viability of tomato cells and of the fungal pathogen Cladosporium fulvum. Physiol. Mol. Plant Pathol. 54:131-143 https://doi.org/10.1006/pmpp.1998.0195
  6. Prasad T.K., M.D. Anderson, and C.R. Stewart. 1994. Acclimation, hydrogen peroxide, and abscisic acid protect mitochondria against irreversible chilling injury in maize seedlings. Plant Physiol. 105:619-627 https://doi.org/10.1104/pp.105.2.619
  7. Sairam, R.K. and D.C. Saxena. 2000. Oxidative stress and antioxidants in wheat genotypes: Possible mechanism of water stress tolerance. J. Agronomy & Crop Science. 184:55-61 https://doi.org/10.1046/j.1439-037x.2000.00358.x
  8. Taub, D.R., J.R. Seemann, and J.S. Coleman 2000. Growth in elevated $CO_{2}$ protects photosynthesis against high-temperature damage. Plant, Cell and Environment 23:649-656 https://doi.org/10.1046/j.1365-3040.2000.00574.x
  9. Weis, E. and J.A. Berry. 1988. Plants and high temperature stress. Experimental Biology. 1004:329-346
  10. Woo, Y.H., H.J. Kim, Y.I. Nam, and Y.S. Kwon. 1998. Analysis of cooling efficiency and required number of air changes and amount of water to set point of inside air temperature at evaporative cooling system in summer glasshouse. RDA. J. Horti. Sci. 40(2):209-215
  11. Woo, Y.H., H.J. Kim, Y.l. Nam, l.H. Cho, and Y.S. Kwon. 2000. Predicting and measuring transpiration based on phytomonitoring of tomato in greenhouse. J. Kor. Soc. Hort. Sci. 41(5):459-463
  12. Woo, Y.H., J.M. Lee, H.J. Kim, and Y.l. Nam. 1995. Forced ventilation number of air changes to set point of inside air temperature in summer glasshouse. J. Bio-Environment Control. 4(2):223-231
  13. Woo, Y.H., J.M. Lee, H.J. Kim, and Y.S. Kwon. 1996. Prediction of maximum air temperature and cooling load of glasshouse during summer. J. Kor. Soc. Hort. Sci. 37(3):479-485
  14. Yamane, K., S. Kawabata, and N. Fujishige. 1999. Change in activities of super oxide dismutase, catalase, and peroxidase during senescence of gladiolus florets. J. Jpn. Soc. Hort. Sci. 68:798-802 https://doi.org/10.2503/jjshs.68.798
  15. Yamane, Y., Y. Kashino, H. Koike, and K. Satoh. 1997. Increases in the fluorescence Fo level and reversible inhitition of Photosystem reaction center by high-temperature treatments in higher plants. Photosynthesis Research 52:57-64 https://doi.org/10.1023/A:1005884717655
  16. Yordanov L., V. Velikova, and T. Tsonev. 2000. Plant responses to drought, acclimation, and stress tolerance. Photosynthetica 38(1):171-186 https://doi.org/10.1023/A:1007201411474