Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Phytate Determination in Various Cultivars of Korean Rice

  • Huang, Long Shuang (College of Pharmacy, Chungnam National University) ;
  • Sok, Dai-Eun (College of Pharmacy, Chungnam National University) ;
  • Kim, Hyoung-Chin (Department of Food and Nutrition, Chungnam National University) ;
  • Yoon, Won-Kee (Department of Food and Nutrition, Chungnam National University) ;
  • Kim, Hwan-Mook (Department of Food and Nutrition, Chungnam National University) ;
  • Kim, Mee-Ree (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2006.03.01
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Abstract

To determine the amount of phytate in rice grains from various cultivars, two methods were employed and compared in respect of the accuracy and conveniency. Phytate in rice samples was extracted with HCl, and then the extracts were subjected to an anion-exchange column. Finally, the phytate in eluate was quantitated using two methods: one method is based on the complex formation between ferric ion and sulfosalicylic acid in the presence of phytate, and the other is the prior acid digestion of phytate sample, followed by the colorimetric determination of liberated phosphorus. Although two methods showed similar values of phytate in rice samples, the former method is simpler and more precise than the latter. Moreover, the former is more reliable for the samples with lower phytate levels. Especially, the dilution condition of rice sample before anion exchange column separation was important for the recovery of phytate in rice samples. Based on the former method, the amount of phytate in rice of various cultivars was estimated to range from 7.3 mg/g to 12.4 mg/g rice. This method would be useful for the determination of phytate in crop samples with a lower level of phytate, one of anti-nutrients in some agricultural plants.

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References

  1. Fruhbeck G, Alonso R, Marzo F, Santidrian S. 1995. A modified method for the indirect quantitative analysis of phytate in foodstuffs. Analytical Biochemistry 225: 206- 212 https://doi.org/10.1006/abio.1995.1145
  2. Dorsch JA, Cook A, Young KA, Anderson JM, Bauman AT, Volkmann CJ, Murthy PP, Raboy V. 2003. Seed phosphorus and inositol phosphate phenotype of barley low phytic acid genotypes. Phytochemistry 62: 691-706 https://doi.org/10.1016/S0031-9422(02)00610-6
  3. Lestienne I, Caporiccio B, Besancon P, Rochette I, Treche S. 2005. Relative contribution of phytates, fibers, and tannins to low iron and zinc in vitro solubility in pearl millet (Pennisetum glaucum) flour and grain fractions. J Agric Food Chem 53: 8342-8348 https://doi.org/10.1021/jf050741p
  4. Lonnerdal B. 2000. Dietary factors influencing zinc absorption. J Nutr 130: 1378-1383
  5. Grases F, Sohnel O, Costa-Bauza A, Ramis M, Wang Z. 2001. Study on concretions developed around urinary catheters and mechanisms of renal calculi development. Nephron 88: 320-328 https://doi.org/10.1159/000046015
  6. Ohkawa T, Ebisuno S, Kitagawa M, Morimoto S, Miyazaki Y, Yasukawa S. 1984. Rice bran treatment for patients with hypercalciuric stones: experimental and clinical studies. J Urol 132: 1140-1145 https://doi.org/10.1016/S0022-5347(17)50065-8
  7. Steer TE, Gee JN, Johnson IT, Gibson GR. 2004. Biodiversity of human faecal bacteria isolated from phytic acid enriched chemostat fermenters. Curr Issues Intest Microbiol 5: 23-39
  8. Pivovarov AS. 1995. Plasticity of cholinoreceptors of neurons of the common snail after effects on inositol-1, 4,5-triphosphate- and Ca$^{2+}$-dependant mobilization of stored Ca$^{2+}$ and the level of phosphatidic acid. Neurosci Behav Physiol 25: 474-482 https://doi.org/10.1007/BF02359275
  9. Wade HE, Morgan DM. 1955. Fractionation of phosphates by paper ionophoresis and chromatography. Biochem J 60: 264-270 https://doi.org/10.1042/bj0600264
  10. Phytate in foods anion-exchange method. 1990. AOAC Offical methods of analysis. 15th ed. Association of official analytical chemists, Washington DC. p 800-801
  11. Munoz JA, Valiente M. 2003. Determination of phytic acid in urine by inductively coupled plasma mass spectrometrty. Anal Chem 75: 6374-6378 https://doi.org/10.1021/ac0345805
  12. Oberleas D, Harland BF. 1977. Nutritional agents which affect metabolic zinc status. Prog Clin Biol Res 14: 11-27
  13. Latta M, Eskin M. 1980. A simple and rapid colorimetric  method for phytate determination. J Agric Food Chem 28: 1313-1315 https://doi.org/10.1021/jf60232a049
  14. Xu P, Price J, Aggett PJ. 1992. Recent advances in methodology for analysis of phytate and inositol phosphates in foods. Prog Food Nutr Sci 16: 245-262
  15. Reddy NR, Pierson MD, Sathe SK, Salunkhe DK. 1989. Phytates in cereals and legumes. BocaReton, FL. p 27-38
  16. Crans DC, Mikus M, Marshman RW, 1993. 31P NMR examination of phosphorus metabolites in the aqueous, acidic, and organic extracts of Phaseolus vulgaris seeds. Anal Biochem 209: 85-94 https://doi.org/10.1006/abio.1993.1086
  17. Oberleas D. 1971. The determination of phytate and inositol phosphates. Methods Biochem Anal 20: 87-101 https://doi.org/10.1002/9780470110393.ch3
  18. Ellis R, Morris ER, Philpot C. 1997. Quantitative de-termination of phytate in the presence of high lnorgainc phosphate. Anal Biochem 77: 536-539 https://doi.org/10.1016/0003-2697(77)90269-X

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