Antioxidant and Anticancer Activities of Extracts from Styela clava According to the Processing Methods and Solvents

가공방법 및 용매에 따른 미더덕 추출물의 항산화 및 항암효과

  • Kim, Jin-Ju (Division of Food Science and Biotechnology, Kyungnam University) ;
  • Kim, Sun-Jung (Division of Food Science and Biotechnology, Kyungnam University) ;
  • Kim, Sun-Hee (Division of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Hae-Ryong (Division of Food Science and Biotechnology, Kyungnam University) ;
  • Lee, Seung-Cheol (Division of Food Science and Biotechnology, Kyungnam University)
  • 김진주 (경남대학교 식품생명공학부) ;
  • 김선정 (경남대학교 식품생명공학부) ;
  • 김선희 (경남대학교 식품생명공학부) ;
  • 박해룡 (경남대학교 식품생명공학부) ;
  • 이승철 (경남대학교 식품생명공학부)
  • Published : 2006.03.01


Styela clava was processed by four different kinds of method including FR (fresh S. clava), H1 (heat treated S. clava at $110^{\circ}C$ for 15 min) H2 (heat treated S. clava at $120^{\circ}C$ for 5 min), and FD (freeze dried S. clava). Each S. clava sample was treated with methanol, ethanol, acetone, and water, then antioxidant and anticancer activities of the extracts were evaluated. In extracts from non-dried S. clava (FR, H1, and H2), total extract yield decreased with increasing treated temperature. The extraction yield was in the order of ethanol>methanol>water>acetone among treated solvents. In case of dried S. clava (FR), the extraction yield was lower than non-dried samples, and was in the order of methanol>ethanol>water>acetone. The radical scavenging activity (RSA) of non-dried S. clava (FR, H1, and H2) was in the order of acetone>ethanol>methanol and heat treatment also decreased RSA. RSA of FD was the highest in ethanol extract, while acetone and water extracts did not show RSA. When antioxidant activity was determined by reducing power (RD), methanol extract of FR showed the highest values and heat treatment decreased RD, too. RD of FD was in the order of methanol>ethanol>water>acetone. The acetone extracts from FD showed significant anticancer activity against human colon cancer cell line HT-29. These results indicated that extraction yield and properties of extracts from S. clava were dependent on processing temperature, solvent and/or physicochemical state. The appropriate extraction process should provide some valuable bioactive materials from S. clava.


  1. Blake D, Winyard PG. 1995. Immunopharmacology of Free Radical Species. Academic Prss, San Diego
  2. Lopaczynski W, Zeissel SH. 2001. Antioxidant, pro-grammed cell death, and cancer. Nutr Res 21: 295-307
  3. Cook NC, Samman S. 1996. Flavonoids chemistry, mechanism, cardioprotective effects and dietary source. Nutr Biochem 7: 66-76
  4. Namiki M. 1990. Antioxidants/antimutagens in food. Crit Rev Food Sci Nutr 29: 273-300
  5. Lindenschmidt RC, Trika AF, Guard ME, Witschi HP. 1986. The effect of dietary butylated hydroxy toluene on liver and colon tumor development in mice. Toxicol 38: 151-160
  6. National Statistical Office. 2004. Annual Report on the Cause of Death Statistics. Seoul, Korea
  7. Kang TB, Liang NC. 1997. Studies on the inhibitory effects of quercetin on the growth of HL-60 leukemia cells. Biochem Pharm 54: 1013-1018
  8. Park KY, Moon SH, Rhee SH, Baek KY, Lim SY. 1995. Effect of tannin from persimmon leaves on the growth inhibition and the synthesis of mRNA of type IV collagen in AZ-521 human gastric cancer cells. Environ Mut Carcino 15: 32-37
  9. Park JC. 1996. Screening of marine natural products on inhibitory effect of the formation of lipid peroxidation. Kor J Pharmacogen 27: 117-122
  10. Jo YG. 1978. The sterol composition of Styela clava. Kor Fish Soc 11: 97-101
  11. Lee KH. 1995. Seasonal variations of nutrients in warty sea squirt (Styela clava). Food Sci Nutr 24: 268-273
  12. Ahn SH. 2003. Extraction of glycosaminoglycans from Styela clava tunic. Biotechnol Bioproc Eng 18: 180-185
  13. Lehrer RI. 2001. Clavanins and styelins, alpha-helical anti-microbial peptides from the hemocytes of Styela clava. Adv Exp Med Biol 484: 71-76
  14. Menzel LP, Lee IH, Sjostrand B, Lehrer RI. 2002. Immunolocalization of clavanins in Styela clava hemocytes. Dev Comp Immunol 26: 505-515
  15. Lee IH, Zhao C, Nguyen T, Menzel L, Waring AJ, Sherman MA, Lehrer RI. 2001. Clavaspirin, an antibacterial and haemolytic peptide from Styela clava. J Pept Res 58: 445-456
  16. Taylor SW, Craig AG, Fischer WH, Park M, Lehrer RI. 2000. Styelin D, an extensively modified antimicrobial peptide from ascidian hemocytes. J Biol Chem 275: 38417- 38426
  17. Lee SC, Kim JH, Jeong SM, Kim DR, Ha JU, Nam KC, Ahn DU. 2003. Effect of far-infrared radiation on the anti-oxidant activity of rice hulls. J Agric Food Chem 51: 4400- 4403
  18. Oyaizu M. 1986. Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 44: 307-315
  19. Goodman GY, Yen YP, Cox TC, Crowlny J. 1987. Effect of verapamil on in vitro cytotoxicity of adriamycin and vin-blastine in human tumor cells. Cancer Res 47: 2295-2311
  20. Fish B. 1984. Clinical trials for the evaluation of cancer therapy. Cancer Res 54: 609-615
  21. SAS Institute. 1995. SAS/STAT User's Guide. SAS In-stitute Inc., Cary, NC
  22. Rim AR, Jo SC, Jung ES, Kim SH, Lee SC. 2005. Study of heating conditions fro long shelf life of Styela clava. J Basic Sci 21: 47-54
  23. Kim JJ, Kim SJ, Kim SH, Park HR, Lee SC. 2005. Anti-oxidant and anticancer activities of extracts from Styela plicata. J Korean Soc Food Sci Nutr 34: 937-941
  24. Yokozawa T, Chen CP, Dong E, Tanaka T, Nonaka GI, Nishioka I. 1998. Study on the inhibitory effect of tannins and flavonoids against the 1,1-diphenyl-2-picrylhydrazyl radical. Biochem Pharm 56: 213-222
  25. Bondent V, Brand-Williams W, Bereset C. 1997. Kinetics and mechanism of antioxidant activity using the DPPH free radical methods. Lebensm Wiss Technol 30: 609-615
  26. Sato A, Shindo T, Kasanuki N, Hasegawa K. 1989. Anti-oxidant metabolites from the tunicate Amaroucium multi-plicatum. J Nat Prod 52: 975-981
  27. Matsuno T, Ookubo M, Komori T. 1985. Carotenoids of tunicates. III. The structural elucidation of two new marine carotenoids, amarouciaxanthin A and B. J Nat Prod 48: 606-613
  28. Cotelle N, Moreau S, Bernier JL, Catteau JP, Henichart JP. 1991. Antioxidant properties of natural hydroquinones from the marine colonial tunicate Aplidium californicum. Free Rad Biol Med 11: 63-68
  29. Choi BD, Kang SJ, Choi YJ, Youm MG, Lee KH. 1994 Utilization of ascidian (Halocynthia roretzi) tunic. 3. Carotenoid compositions of ascidian tunic. Bull Korean Fish Soc 27: 344-350
  30. El-Agamey A, Lowe GM, McGarvey DJ, Mortensen A, Phillip DM, Truscott TG, Young AJ. 2004. Carotenoid radical chemistry and antioxidant/pro-oxidant properties. Arch Biochem Biophys 430: 37-48
  31. Meir S, Kanner J, Akiri B, Philosoph-Hadas S. 1995. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J Agric Food Chem 43: 1813-1819
  32. Diplock AT. 1997. Will the good fairies please prove to us that vitamin E lessens human degenerative disease? Free Rad Res 27: 511-532
  33. Jung BM, Lim SS, Park YJ, Bae SJ. 2005. Inhibitory effects on cell survival and quinone reductase induced activity of Aster yomena fractions on human cancer cells. J Korean Soc Food Sci Nutr 34: 8-12
  34. Choe WK, Park JH, Kim SH, Lee DY, Lee YC. 1999. Anti-tumor effects of green tea catechin on different cancer cells. Korean J Nutr 32: 838-843

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