Production of Lignin Degrading Enzymes and Decolorization of Various Dye Compounds by Wood-Rot Fungi

목재 부후균의 리그닌 분해효소 활성과 염료 화합물의 탈색

  • Published : 2006.03.01

Abstract

Wood-rot fungi produce extracellular lignin-degrading enzymes, the best known of which are lignin peroxidase, Mn-peroxidase and laccase. In this experiment, some of them produced all of three enzymes. Many other wood-rot fungi produced one or two of those enzymes with various combinations. In this experiment, we tried to clarify the relationship between the pattern of enzyme production and degradative activity of several dye compounds. From the 36 strains of 23 species of wood-rot fungi, Mn-peroxidase activity was found in 30 strains of the fungi tested, whereas the activity of lignin peroxidase and laccase was detected in 11 strains and 12 strains of species, repectively, in Kirks low nitrogen media. In relation to the activity of lignin degrading enzymes and degradation of dye compounds, the white-rot fungi with three kinds of enzymes tested showed the best dye decolorizers. The fungi with Mn-peroxidase activity only decolorized poly R-478 and remazol brilliant blue R dye in proportion to the enzyme activity, while methylene blue, bromophenol blue and congo red dye were degraded in regardless of enzyme activity. Those dyes were degraded in relation to the growth rate of mycelium. Brown-rot fungi did not degrade all the dye compounds except bromophenol blue, in spite of moderate growth rate.

목재부후균은 리그닌 분해효소로 lignin peroxidase (LIP), Mn-peroxidase (MNP) 및 laccase를 생성하는데 균류에 따라 위의 효소중 하나 혹은 둘 이상의 효소를 분비하거나 전혀 생성하지 않는 균도 있다. 본 실험은 이러한 목재 부후균의 효소생성 양상과 몇 종의 염료화합물 탈색과의 상관관계를 조사하고자 하였다. 조사한 23종 36균주 중 MNP 생성균은 30균주였으며 LIP 혹은 laccase 생성균은 각각 11균주와 12균주였다. 또한 같은 종에서도 효소활성은 다양한 양상을 보여 주었다. 리그닌 분해효소 활성과 비교하여 염료 탈색 정도는 세 효소가 모두 분비되는 백색 부후균의 경우 염료 탈색율이 상대적으로 우수하였고 균주에 따라 차이가 있으나 MNP 활성만을 갖는 균주의 경우, poly R-478 polymeric dye 및 anthron-type dye 인 remazol brilliant blue R염료는 효소 활성도와 다소 유연관계를 보였으며 methylene blue, bromophenol blue및 congo red 염료는 위의 효소들과는 직접적인 관련이 없는 것으로 판단되었으며, 오히려 균사의 생장과 비례하여 탈색율을 나타냈다. LIP, MNP 및 laccase 효소활성이 거의 검출되지 않은 갈색 부후균에서는 bromophenol blue를 제외하고는 염료의 탈색이 10%미만 혹은 전혀 탈색이 되지 앓았다.

Keywords

References

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