Elastomers and Composites

The Rubber Society of Korea (한국고무학회)
권호 표지

Electrochemical Properties of Biosensor with Butyl Rubber Binder

부틸고무를 결합재로 사용한 바이오센서의 전기화학적 성질

  • Published : 2006.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

An enzyme electrode bound by butyl rubber was newly constructed for the determination of hydrogen peroxide and for the practical application as a biosensor. Then its electrochemical properties were investigated. It produced a hundreds-fold increased signal compared to the plant or animal tissue based biosensor studied previously and could be run at between $0.0{\sim}-1.00\;V$(vs. Ag/AgCl). The relationship between signal and electrode potential was linear in the experimental range of potential. It showed a detection limit of $3.0{\times}10^{-4}\;M$ and a very good linearity of Lineweaver-Burk plot giving the proof of a good enzyme immobilization. Especially, both the reproducibility of signal current due to its high sensitivity and mechanical stability presented a new possibility for the practical use of biosensor bound with butyl rubber.

부틸고무를 결합재로 사용하여 과산화수소 정량 바이오센서를 제작하고, 그것의 전기화학적 특성과 실용화 가능성을 살펴보았다. 전극은 본 실험실에서 연구되었던 실리콘 오일을 결합재로 사용한 동식물조직 바이오센서보다 수백 배 이상 큰 신호를 보여주었으며, $0.0{\sim}-1.00\;V$(vs. Ag/AgCl)의 넓은 퍼텐셜 영역에서 안정적으로 작동될 수 있었다. 또 신호전류는 실험 퍼텔셜 영역에서 전극전위에 따라 좋은 직선성을 보였다. 검출한계는 $3.0{\times}10^{-4}\;M$ 이었으며, Lineweaver-Burk 도시의 직선성은 효소가 전극 표면에 효과적으로 고정되어 있음을 확인시켜 주었다. 높은 감도에 의한 신호전류의 재현성과, 부틸고무의 결합력에 의한 전극물질의 기계적 안정성은 전극의 실용화 가능성을 보여 주었다.

Keywords

References

  1. C. G. J. Koopal, M. C. Feiters, R. J. M. Nolte, B. de Ruiter, and R. B. M. Schasfoort, 'Third-generation Amperometric Biosensor for Glucose. Polypyrrole Deposited within a Matrix of Uniform Latex Particles as Mediator', Bioelectrochem. Bioenerg. 29, 159 (1992) https://doi.org/10.1016/0302-4598(92)80064-N
  2. S. Cosnier, S. Szunerits, R. S. Marks, A. Novoa, L. Peuch, E. Perez, and I. Rico-Lattes, 'A Rapid and Easy Procedure of Biosensor Fabrication by Microencapsulation of Enzyme in Hydrophilic Synthetic Latex Films. Application to the Amperometric Determination of Glucose', Electrochem. Comm. 2, 851 (2000) https://doi.org/10.1016/S1388-2481(00)00135-1
  3. M. G. Roig, F. B. Estevez, F. G. Velasco, N. I. Ghais, and .I. M. C. Silverio, 'Biotechnology and Applied Biology Section, Methods for Immobilizing Enzymes', Biochem. Educ., 14, 180 (1986) https://doi.org/10.1016/0307-4412(86)90213-X
  4. M. S. Lin, S. Y. Tham, and G. A. Rechnitz, 'Pineapple-Tissue Based Bioelectrode for the Determination of Hydrogen Peroxide', Electroanalysis, 2, 511 (1990) https://doi.org/10.1002/elan.1140020703
  5. Y. Fang, R. Cai, J. Deng, and Z. Deng, 'Lettuce Seed Meal Tissue-Based Membrane Electrode with High Biocatalytic Activity for Hydrogen Peroxide', Electroanalysis, 4, 819 (1992) https://doi.org/10.1002/elan.1140040813
  6. B. Li, Z. Zhang, and Y. Iin, 'Plant Tissue Based Chemiluminescence Flow Biosensor for Determination of Unbound Dopamine in Rabbit Blood with On-line Microdialysis Sampling', Biosensors & Bioelctronics, 17, 585 (2002) https://doi.org/10.1016/S0956-5663(02)00022-2
  7. K. J. Yoon, H. S. Kwon, and B. G. Lee, 'Electrochemical Determination of pH-stability Curve of Enzyme', J. Kor. Chem. Soc. 49(3), 325 (2005) https://doi.org/10.5012/jkcs.2005.49.3.325
  8. H. S. Kwon, E. H. lin, K. J. Yoon, and Y. N. Pak, 'Mushroom-juice Based Gold Electrode for the Determination of Phenols', J. Kor. Chem. Soc. 49(2), 224 (2005) https://doi.org/10.5012/jkcs.2005.49.2.224
  9. J. Wang, N. Naser, H. S. Kwon, and M. Y. Cho, 'Tissue Bioelectrode for Organic-phase Enzymatic Assays', Anal. Chim. Acta 264, 7 (1992) https://doi.org/10.1016/0003-2670(92)85290-M
  10. K. J. Yoon, 'Development of Biosensor Utilizing Rubber', Bull. Kor. Chem. Soc. submitted (2006)
  11. B. Wang and S. Dong, 'Organic-phase Enzyme Electrode for Phenolic Determination Based on a Functionalized Sol-gel Composite', J. Electroanal. Chem. 487, 45 (2000) https://doi.org/10.1016/S0022-0728(00)00152-2
  12. K. J. Yoon, 'A New Strategy for Determining Optimum pH of Isozyme', Bull. Kor. Chem. Soc. 25(7), 997 (2004) https://doi.org/10.5012/bkcs.2004.25.7.997
  13. A. N. Diaz, M. C. R. Peinado, and M. C. T. Minguez, 'Sol-gel Horseradish Peroxidase Biosensor for Hydrogen Peroxidase Detection by Chemiluminescence', Anal. Chim. Acta, 363, 221 (1998) https://doi.org/10.1016/S0003-2670(98)00080-4
  14. Y. Miao, and S. N. Tan, 'Arnperometric Hydrogen Peroxide Biosensor with Silica Sol-gel/Chitosan Film as Immobilization Matrix', Anal. Chim. Acta, 437, 87 (2001) https://doi.org/10.1016/S0003-2670(01)00986-2