Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effects of Straw Size and Thawing Rate on Post-thaw Quality of Bog Semen

개의 동결 정액 제조시 Straw의 크기와 융해온도가 정자의 생존율에 미치는 영향

  • Son J.M. (College of Veterinary Medicine, Chungnam National University) ;
  • Kim Y.S. (College of Veterinary Medicine, Chungnam National University) ;
  • Shin Y.J. (College of Veterinary Medicine, Chungnam National University) ;
  • Lim Y.H. (College of Veterinary Medicine, Chungnam National University) ;
  • Yoon K.Y. (Department of Animal Science, Shingu College) ;
  • Lee D.S. (College of Veterinary Medicine, Chungnam National University) ;
  • Shin S.T. (College of Veterinary Medicine, Chungnam National University) ;
  • Cho J.K. (College of Veterinary Medicine, Chungnam National University)
  • Published : 2006.03.01
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Abstract

본 연구는 개 동결 정액 융해 시 straw 크기 및 융해 속도가 융해 정자의 질(quality)에 미치는 영향을 조사하고 최적의 융해 조건을 조사하는데 그 목적이 있다. 정상적인 번식능을 가진 비글 수컷 5마리에서 정액을 채취하여 원심 분리하여 정장을 버리고 남은 정자에 동결보호제인 glycerol이 첨가된 tris-glucose-egg yolk extender를 첨가하여 동결하고 액체질소에 보관한 후 융해하였다. 동결 융해 조건에 따른 효과를 알아보기 위해 straw는 0.25 ml과 0.5 ml크기를 사용하였고 융해 조건은 $75^{\circ}C$에 10초, $55^{\circ}C$에 12초 및 $37^{\circ}C$에서 120초로 하여 융해 후 정자의 활력도(vigor), 운동성(motility), Hypo-osmotic test(HOS test)를 이용한 생존성(viability) 및 $SperMac^{\circledR}$ 염색을 하여 정자의 membrane integrity를 비교 조사하였다. 조사 결과 0.5 ml 크기의 straw를 사용한 경우 $37^{\circ}C$ 융해가 $55^{\circ}C,\;75^{\circ}C$ 융해보다, 0.25 ml 크기의 straw를 사용한 경우에는 $37^{\circ}C,\;55^{\circ}C$ 융해가 $75^{\circ}C$ 융해보다 유의적으로 높은 활력 지수 및 생존성을 보였다(P<0.05). Straw크기에 따라 비교하였을 경우 0.5 ml 군에서 유의적으로 높은 활력도, 생존성 및 membrane integrity를 보였다(P<0.05). 결론적으로 개 정액이 동결 및 융해 시 0.5ml straw를 이용하여 동결한 후 $37^{\circ}C$에서 120초 동안 융해하는 것이 최적의 조건임이 사료된다.

Keywords

References

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