Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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The Effect of Ginseng and Caffeine Products on the Antioxidative Activities of Mouse Kidney

인삼과 카페인 함유제품이 흰쥐 신장의 항산화활성에 미치는 영향

  • Chang, Young-Sang (Department of Food Science and Biotechnology, Joongbu University) ;
  • Chang, Yoon-Hyuk (Department of Food Science and Human Nutrition, Michigan State University) ;
  • Sung, Jong-Hwan (Central Research Institute, Il Hwa Co., Ltd.)
  • 장영상 (중부대학교 식품생명공학과) ;
  • 장윤혁 (미시간주립대학교 식품영양과학과) ;
  • 성종환 ((주)일화 중앙연구소)
  • Published : 2006.03.01
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Abstract

This study was carried out to determine the antioxidative activities of tissue cells of mouse kidney isolated from mice fed with different formulation of drinks. The swimming ability of experimental groups without caffeine showed the same pattern, but caffeine group decreased slightly. Superoxide dismutase(SOD) and catalase(CAT) activities of caffeine-containing groups were decreased significantly, but the control and ginseng-containing groups were increased. Hydroperoxide contents did not increase significantly all experimental groups. Hydroperoxlde contents of the control and ginseng-containing groups were similar, but caffeine-containing groups increased. Compared to the control and ginseng-containing groups, lipid peroxidation level and protein contents in caffeine-containing groups were decreased significantly. In conclusion, the antioxidative activities of mouse kidney isolated from mice fed with ginseng-containing products was increased slightly.

인삼성분과 카페인 함유제품을 마우스에 섭취시켜 4주간사육한 후, 마우스 신장 조직에서 Superoxide dismutase, Catalase, Hydroperoxide 등의 항산화 활성 및 조직학적 변화를 비교 검토하였다. 유영능력은 모든 실험군에서 유의적인 차이를 보이지 않았으나 무수카페인 실험군만은 다소 감소하는 경향을 보였다. Superoxide dismutase 및 Catalase 활성은 카페인함유 실험군에서 유의적으로 감소하였으나, 인삼성분함유 실험군에서는 활성도가 높게 나타났다. Hydroperoxide 함량은 모든 실험군에서 유의적인 증가를 보이지 않았으나 인삼성분함유 실험군은 대조군과 비슷한 함량을, 카페인함유 실험군은 대체로 증가하는 경향이었다. 과산화 지질 수준 및 단백질 함량 변화는 인삼성분 실험군은 대조군에 비하여 유의성 있게 증가하지 않았으나 카페인 함유제품 실험군은 유의성 있는 증가를 보였다. 신장 조직학적 변화는 인삼성분 및 카페인 함유 실험군에서 크나큰 조직적 차이를 보이지 않았다. 따라서 각 실험군과 실험방법에 따라 차이는 있겠으나 본 실험에 있어서는 인삼성분 함유제품이 카페인 함유제품보다 신장 조직의 항산화활성능력이 다소 증가하는 것으로 생각된다.

Keywords

References

  1. Park, M. G. : Korean ginseng. Korea Ginseng and Tobacco Research institute, Seoul Korea, p.63 (1994)
  2. Lee, D. W., Sohn, H. O., Lim, H. B. and Lee, Y. G. : Antioxidant action over ginseng. Korean J. Ginseng Sci. 19, 31 (1995)
  3. Sung, J. H. : Effects of white and red panax ginseng extract on serum lipids level and antioxidative activities in high-fatdiet fed rats. Ph.D. thesis, Kunsan National University. (2005)
  4. Kim, S. S., Kim, J. D., Kim, H., Shin, M. S., Park, C. K., Park, H. M. and Yang, J. W. : The effects on the blood lipid profiles and body fat by long term administration of red ginseng product. J. Ginseng Res. 26, 67-73 (2002) https://doi.org/10.5142/JGR.2002.26.2.067
  5. Tonychou, M. D. : Wake up and smell the coffee-caffeine, coffee and the medical consequences. West. J. Med. 157, 533-544 (1992)
  6. Regestein, Q. R. : Pathologic sleepiness induced by caffeine. Am. Med. 588, 425-429 (1981)
  7. Goldsteine, A., Warren, R. and Kaizer, S. : Psychotropic effects of caffeine I. Individual difference in sensitivity of caffeine-induced wakefulness. J. Pharmacol. Exp. Ther. 419, 156-159 (1965)
  8. Lee, H. W. : A study on caffeine containing foods and the effect of caffeine in humans. Culinary Research. 6, 343-355 (2000)
  9. Williams, M. H. : Nutritional ergogenic acids and athletic performance. Nutrition Today. Jan/Feb. 7-14 (1989)
  10. Ha, J. H., Yeum, C. H., Kim, S. W., Kim, N. H., Choi, K. C. and Lee, J. U. : Enhanced atrial natriuretic peptide and nitricoxide system following the treatment with caffeine in rats. Korean J. Nephrology. 21, 123-128 (2002)
  11. Fenster, L., Eskenazi, B., Windham, G. C. and Swan, S. H. : Caffeine consumption during pregnancy and fetal growth. Am. J. Pub. Health. 81, 458-461 (1991) https://doi.org/10.2105/AJPH.81.4.458
  12. Lecos, C. W. and Caffeine, J. : Some safety questions remain. FDA Consumer Dec. p. 22-27 (1987)
  13. Rothore, N., John, S., Kale, M. and Bhatnagar, D. : Lipid peroxidation and antioxidant enzymes in losproterenol induced oxidative stress in rat tissues. Phamacological Research. 38, 197-303 (1998)
  14. Jeon, B. H., Seong, G. S., Sung, J. H. and Chang, C. C. : Antioxidative effects of white ginseng and red ginseng on liver of high fat diet-treated mice. J. Ginseng Res. 29, 138-144 (2005) https://doi.org/10.5142/JGR.2005.29.3.138
  15. Sung, J. H., Chang, C. C. and Chang, Y. S. : The effect of caffeine on the antioxidative of mouse liver. Korean J. Food & Nutr. 17, 442-449 (2004)
  16. Jung, K. and Henke, W. : Development changes of antioxidant enzymes in kidney and liver from rats. Free Radical Biology and Medicine. 20, 613-617 (1996) https://doi.org/10.1016/0891-5849(95)02090-X
  17. Yokozawa, T., Liu, Z. W. and Dong, E. : A study of ginsenoside-Rd in a renal ischemia-reperfusion model., Naphron Basel. 78, 201-206 (1998)
  18. Deng, H. L. and Zhang, J. T. : Anti-lipid peroxidative effect of ginsenoside $Rb_1\;and\;Rg_1$, Chin. Med. J. Med. 104, 395-398 (1991)
  19. Flohe, L. and Otting, F. Superoxide dismutase assays. Methods in Ezymology. 105, 93-105(1984) https://doi.org/10.1016/S0076-6879(84)05013-8
  20. Aebi, H. E. : Catalase.In;Method of enzymatic an analysis. Bergmyer, H. U. erlag. Chemi. Weinheim. 3, 273-286 (1982)
  21. Wolff, S. P. : Hydrogen peroxide assays. Methodsin Enzymology, 233, 182-186 (1994) https://doi.org/10.1016/S0076-6879(94)33021-2
  22. Ohkawa, H, Ohishi, N. and Yagi, K. : Assay for lipid peroxides in animal tissues bythiobarbituric acid reaction. Analytical Biochem. 95, 351-358 (1979) https://doi.org/10.1016/0003-2697(79)90738-3
  23. Bradford, M. M. : A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. J. Anal. Biochem. 72, 248-254 (1976) https://doi.org/10.1016/0003-2697(76)90527-3
  24. Manual of histologic and special staining technology. 2nd ed. 32. Mcgraw-hill book co. New york (1960)
  25. Schefler, W. C. : Statistics for the biological sciences. Addison-wesley, London (1980)
  26. Klaus, J. and Wolfgang, H. : Development changes of antioxidant enzymes in kidney and liver from rats. Free radical biology & medicine. 20, 613-617 (1996) https://doi.org/10.1016/0891-5849(95)02090-X
  27. Callahan, M. M., Rohovsk, M.W., Robertson, R. S. and Yesair, D. W. : The effect of coffee consumption on plasma lipids, lipoproteins, and the development of aortic atherosclerosis inthesus monkeys fed an atherogenic diet. Am. J. Clin. Nutr. 32, 834-548 (1979) https://doi.org/10.1093/ajcn/32.4.834
  28. Thelle, D. S., Arnesen, E. and Forde, O. H. : The tromso heart study. Does coffee raise serum cholesterol? N. Engl. J. Med. 308, 1454-1457 (1983) https://doi.org/10.1056/NEJM198306163082405
  29. Park, M. L. and Cho, S. Y. : Effects of dietary vitamin E level and caffeine on lipid peroxidation in rat liver. J. Korean Soc. Food Nutr. 23, 561-567 (1994)
  30. Bei, H. S., Lee, S. K. and Ahn, H. S. : Effect of caffeine on lipid metabolism in the rat fed with different levels of dietary lipids. J. Basic Science, Sung shin women's univ. 4, 1-11 (1987)

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