Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Bone formation effect of $HA/{\beta}-TCP$ composite powders in rabbit calvarial bone defects;Histologic study

토끼의 두개골내에 형성된 골결손부에서 $HA/{\beta}-TCP$ composite powders의 골형성에 관한 조직학적 연구

  • Lee, Kwang-Ho (Department of Periodontology, College of Dentistry, Chosun University) ;
  • Jang, Hyun-seon (Department of Periodontology, College of Dentistry, Chosun University, Oral Biology Research Institute, Chosun University) ;
  • Park, Joo-Cheol (Department of Oral Histology, College of Dentistry, Chosun University, Oral Biology Research Institute, Chosun University) ;
  • Kim, Heung-Joong (Department of Oral Anatomy, College of Dentistry, Chosun University, Oral Biology Research Institute, Chosun University) ;
  • Kim, Chong-Kwan (Department of Periodontology, College of Dentistry, Yonsei University) ;
  • Kim, Byung-Ock (Department of Periodontology, College of Dentistry, Chosun University, Oral Biology Research Institute, Chosun University)
  • 이광호 (조선대학교 치과대학 치주과학 교실) ;
  • 장현선 (조선대학교 치과대학 치주과학 교실, 조선대학교 치과대학 구강생물학연구소) ;
  • 박주철 (조선대학교 치과대학 구강조직학 교실, 조선대학교 치과대학 구강생물학연구소) ;
  • 김흥중 (조선대학교 치과대학 구강해부학 교실, 조선대학교 치과대학 구강생물학연구소) ;
  • 김종관 (연세대학교 치과대학 치주과학 교실) ;
  • 김병옥 (조선대학교 치과대학 치주과학 교실, 조선대학교 치과대학 구강생물학연구소)
  • Published : 2006.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of the present study was to evaluate the histologic results of bone cavities that were surgically created in the calvaria of rabbit and filled with $HA/{\beta}-TCP$ composite powders, which had been developed in Korea (Dentium, Korea). Ten young adult rabbits were used. Four defects were surgically produced in calvaria of each rabbit. Each rabbit was anesthetized with Ketamine-HCI (5 mg/kg, Yuhan Cor. Korea) and Xylazine-HCI (1.5 ml/kg, Yuhan Cor. Korea)). An incision was made to the bony cranium and the periosteum was reflected. Using a trephine bur (external diameter: 8 mm, 3i, USA), 4 'through-and-through' bone defects were created with copious irrigation, and classified into 4 groups: control group: no graft materials, experimental group I: normal saline + graft materials: experimental group II: venous blood + graft materials: experimental group III: graft materials only. The defects were randomly filled with graft materials. The defects were closed with resorbable suture material. At the end of the surgical procedure, all animals received a single intramuscular injection of antibiotics Gentamicin (0.1 mg/kg, Dae Sung Microb. Korea). Rabbits were sacrificed with phentobarbital (100 mg/kg) intravenously at 1-, 2-, 4-, 6- and 8-week after. Specimens were treated with hydrochloric acid decalcifying solution (Fisher Scientific, Tustin, CA) and sectioned by bisecting the 8 mm diameter defects. The histologic specimens were prepared in the general method with H & E staining at 6 ${\mu}m$ in thickness. The results were as follows; 1. New bone formation showed from after 2-week of surgery in defect area. As time lapsed, lots of new bone formation and mature bones showed. 2. Histologically, degree of new bone formation could not be discerned among the experimental groups. But, for experimental group II, lots of cells gathered around graft materials after 1-week of surgery, new bone formed slightly faster and than the others at 1-week after. For experimental group I, a few inflammatory finding showed around graft material at after 1-week and after 2-week of surgery. 3. No bone formation did show for control group. Based on histologic results, the new $HA/{\beta}-TCP$ composite powders appeared to act as a scaffolding material for regeneration of osseous defects.

Keywords

References

  1. 김병옥. 골이식을 위한 치주치료의 최신지견. 대한치과의사협회지. 2005, 43(12): 768-773
  2. 김현수. BBP가 치주 골내낭 치유에 미치는 영향. 대한치주과학회지 2002, 40(1):213-223
  3. Bagot D'arc M., Daculsi G. Micro macroporous biphasic ceramics and fibrin sealant as moldable material for bone reconstruction in chronic otitis media surgery. A 15 years experience. Kluwer Academc Publishers. 2003
  4. Cancian DCJ, Hochuli-Veira E. Marcantonio RAC, et al. Utilization of autogenous bone. bioactive glasses. and calcium phosphate cement in surgical mandibular bone defects in cebus apella monkeys. INT J ORAL MAXILLOFAC IMPLANTS. 2004. 19:73-79
  5. de Groot K. Clinical applications of calcium phosphate biomaterials: a review. Ceramics International. 1993. 19:363-366 https://doi.org/10.1016/0272-8842(93)90050-2
  6. Dupraz A, Nguyen TP, Richar M, et al. Influence of a cellulosic ether carrier on the structure of biphasic calcium phosphate ceramic particles in an injectable composite material. Biomaterials, 1999. 20:663-673 https://doi.org/10.1016/S0142-9612(98)00222-1
  7. Ellinger RF, Nery EB, Lynch KL. Histological assessment of periodontal osseous defects following implantation of hydroxyapatite and biphasic calcium phosphate ceramic. A case report. Int J Periodontics Restorative Dent, 1986. 6(3) :22-33
  8. Froum SJ, Kushner L, Scopp IW, et al. Human clinical and histologic responses to durapatite implants in intraosseous lesions. J Periodontol, 1982. 53(12): 719-725 https://doi.org/10.1902/jop.1982.53.12.719
  9. Gauthier O, Bouler JM, Aguado E, et al. Macroporous biphasic calcium phosphate ceramics: influence of macropore diameter and macroporosity percentage on bone ingrowth. Biomaterials. 1998. 19: 133-139 https://doi.org/10.1016/S0142-9612(97)00180-4
  10. Gauthier O, Biox D, Grimanoi G, et al. A new injectable calcium pphosphate biomaterial for immediate bone filling of extraction sockets: A preliminary study in dogs. J Periodontol, 1999, 70:375-383 https://doi.org/10.1902/jop.1999.70.4.375
  11. Jarcho M. Biomaterial aspect of calcium phosphates (properties and applicaions). Dent Clin Nor Am, 1986, 30:25-47
  12. Kim Ck, Choi EJ, Cho KS et al. Periodontal repair in intrabony defects treated with a calcium carbonate implant and guided tissue regeneration. J Periodontol 1996, 67: 1301-1306 https://doi.org/10.1902/jop.1996.67.12.1301
  13. Kwon SH, Jun YK, Hong SH et al. Synthesis and dissolution behavior of B-TCP and $HA/{\beta}-TCP$ composite powders. J. Euro. Ceram. Soc., 2003, 23:1039-1045 https://doi.org/10.1016/S0955-2219(02)00263-7
  14. Lee YM, Park YJ, Lee SJ, et al. Tissue engineered bone formation using chitosan/tricalcium phosphate sponges. J Periodontol, 2000, 71 :410-417 https://doi.org/10.1902/jop.2000.71.3.410
  15. Lee YM, Park YJ, Lee SJ, et al. The bone regenerative effect of platelet-derived growth factor-BB delivered with a chitosan/tricalcium phosphate sponge carrier. J Periodontol, 2000, 71:418-424 https://doi.org/10.1902/jop.2000.71.3.418
  16. Levin MP Getter L, Adrian J et al. Healing of periodontal defects with ceramic implants. J Clin Periodontol, 1974, 1:197-205 https://doi.org/10.1111/j.1600-051X.1974.tb01258.x
  17. Monroe EA, Votava W, Bass DB et al. New calcium phosphate ceramic material for bone and tooth implants. J Dent Res, 1971, 50:860-861 https://doi.org/10.1177/00220345710500041201
  18. Moskow BS, Bubarr A. Histological assessment of human periodontal defects after durapatite ceramic implants. J Periodontol, 1983, 54:455-462 https://doi.org/10.1902/jop.1983.54.8.455
  19. Nery EB, LeGeros RZ, Lynch KL, et al 'Tissue response to biphasic calcium phosphate ceramic with different ratios of $HA/{\beta}-TCP$ in periodontal osseous defects, J Periodontol, 1992, 63: 729-735 https://doi.org/10.1902/jop.1992.63.9.729
  20. Position paper. Periodontal regeneration. J Periodontol, 2005. 75: 1601-1622
  21. Rabalais ML, Yukna TA, Mayer ET, Evaluation of durapatite ceramic as an alloplastic implant in periodontal osseous defects. I Initial six month results. J Periodontol, 1981. 52(11): 680-689 https://doi.org/10.1902/jop.1981.52.11.680
  22. Reynolds MA, Aichelmann-Reudt ME, Branch-Mays GL, et al. The efficacy of bone replacement grafts in the treatment of periodontal osseous defects. A systematic review. Ann Periodontol, 2004, 227-265
  23. Saffar JL, Colombier ML, Detienville R. Bone formation in tricalcium phosphate-filled periodontal intrabony lesion. Histological observations in humans. J Periodontol, 1990, 61:209-216 https://doi.org/10.1902/jop.1990.61.4.209
  24. Takeishi A, Hayashi H, Kamatsubara H, et al. Implant of calcium phosphate ceramics altering Ca/P ratio in bone. J Dent Res, 1989, 68:680
  25. Trecant M, Delecrin T. Mechanical changes in macroporous calcium phosphate ceramics after implantation in bone. Clinical Materials, 15:233-240, 1994 https://doi.org/10.1016/0267-6605(94)90051-5
  26. Wiltfang J, Schlegel KA, Schultze-¬Mosgau S, et al. Sinus floor augmentation with ${\beta}-tricalcium$ phosphate $({\beta}-TCP):$ does platelet-rich plasma promote its osseous integration and degradation?. Clin Oral Impl Res, 14:213-218, 2003 https://doi.org/10.1034/j.1600-0501.2003.140212.x
  27. Yukna RA, Harrison BG, Caudill RF, et al. Evaluation of durapatite ceramic as an alloproastic implant in periodontal osseous defects. II. Twelve month reentry results. J Periodontol, 56(9): 540-547, 1985 https://doi.org/10.1902/jop.1985.56.9.540