Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Role of MAP kinase on MMP-13 expression in rat periodontal ligament cells

백서 치주인대세포에서의 MMP-13 mRNA에 대한 MAP kinase의 역할

  • Chung, Chan-Gil (Department of Periodontology School of Dentistry, Chonnam National University) ;
  • Cui, De-Zhe (Dental Science Research Institute, Chonnam National University) ;
  • Chung, Hyun-Ju (Department of Periodontology School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Kim, Young-Joon (Department of Periodontology School of Dentistry, Dental Science Research Institute, Chonnam National University)
  • 정찬길 (전남대학교 치의학전문대학원 치주과학교실) ;
  • 최득철 (전남대학교 치의학전문대학원 치의학연구소) ;
  • 정현주 (전남대학교 치의학전문대학원 치주과학교실 및 치의학연구소) ;
  • 김영준 (전남대학교 치의학전문대학원 치주과학교실 및 치의학연구소)
  • Published : 2006.03.30
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Abstract

Matrix metalloproteinases (MMPs)는 치주조직내에 존재하는 세포외기질의 유지와 분해에 중요한 역할을 담당하고 있으며 이중 MMP-13은 치주질환의 진행과 깊은 관계가 있다고 알려져 있다. 이번 연구는 치주질환의 진행에 있어서 MMP-13의 활성에 대한 mitogen activated protein(MAP) Kinase의 역할을 구명하기 위해 시행되었다. 백서 치주인대세포에서의 MMP-13 mRNA의 발현은 RT-PCR에 의하여, 그리고 MAP Kinase의 발현은 Western blot에 의하여 측정하였다. $Interleukin-1{\beta}$(IL $-1{\beta}$), Tumor necrosis $factora(TNF-{\alpha})$와 parathyroid hormon(PTH)는 MMP- 13 mRNA 발현을 각각 320%, 180%, 380% 증가시켰으나 bone morphogenetic protein-7(BMP-7)은 MMP-13 mRNA의 발현을 증가시키지 않았다. p38 MAP Kinase 억제제인 SB203580은 IL $-1{\beta}$ 유도 MMP-13의 발현을 약 40% 정도 억제시켰으나, PTH-유도 MMP-13 mRNA의 발현은 억제하지 못했다. IL $-1{\beta}$는 MMP- 13 mRNA의 반감기를 약 2시간 정도로 증가시켰으나, p38 MAP Kinase 억제제로 전처치한 경우에는 반감기가 60분으로 줄어들었다. $IL-1{\beta}$는 p38 MAP kinase와 JNK의 인산화 활성을 증가시켰으나 PTH, $TNF-{\alpha}$와 BMP-7은 p38, JNK, ERK의 활성을 증가시키지 못했다. 이상의 연구결과는 p38 MAP Kinase가 백서 치주인대세포에서의 MMP-13 mRNA 발현을 조절하는데 중요한 역할을 담당함을 시사하였다.

Keywords

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