Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Histologic evaluation of the regenerated bone using bone graft materials

수종의 골이식재를 이용한 유도재생골의 조직학적 평가

  • Ryu, Ho-Chul (Department of Periodontology, School of Dentistry, Kyung Hee University) ;
  • Park, Joon-Bong (Department of Periodontology, School of Dentistry, Kyung Hee University) ;
  • Kwon, Young-Hyuk (Department of Periodontology, School of Dentistry, Kyung Hee University) ;
  • Herr, Yeek (Department of Periodontology, School of Dentistry, Kyung Hee University) ;
  • Chung, Jong-Hyuk (Department of Periodontology, School of Dentistry, Kyung Hee University) ;
  • Jue, Seong-Suk (Department of Oral Anatomy, School of Dentistry, Kyung Hee University)
  • 유호철 (경희대학교 치과대학 치주과학교실) ;
  • 박준봉 (경희대학교 치과대학 치주과학교실) ;
  • 권영혁 (경희대학교 치과대학 치주과학교실) ;
  • 허익 (경희대학교 치과대학 치주과학교실) ;
  • 정종혁 (경희대학교 치과대학 치주과학교실) ;
  • 주성숙 (경희대학교 치의학전문대학원 구강해부학교실)
  • Published : 2006.06.30
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Abstract

This study was performed to evaluate the effect of bone graft materials including demineralized freeze-dried bone, freeze-dried bone, deproteinized bovine bone on space-making capacity and bone formation in guided bone regeneration with titanium reinforced ePTFE membrane(TR-ePTFE). Adult male rabbits(mean BW 2kg) were used in this study. Intramarrow penetration defects were surgically created with round bur on calvaria of rabbits. TR-ePTFE membrane was adapted to calvarial defect and bone graft materials were placed. Animals were sacrificed at 2, 8, 12 weeks after surgery. Non-decalcified specimens were processed for histologic analysis and prepared with Villaneuva bone stain. The results of this study were as follows: 1. TR-ePTFE membrane was biocompatible and capable of maintaining the space-making. 2. Tissue integration was not good at TR-ePTFE membrane. Fixation was not enough. so, wound stabilization was not good. 3. In animals using deproteinized bovine bone, demineralized freeze-dried bone, bone formation was little. 4. In animals using freeze-dried bone, bone formation was better. Within the above results, bone formation may be inhibited when wound stabilizafion was not good.

Keywords

References

  1. Dahlin C, Alberius P, Lindhe A. Osteopromotion for cranioplasty. An experimental study in rats using a membrane technique. J Neurosurg 1991;74:487-497 https://doi.org/10.3171/jns.1991.74.3.0487
  2. Dahlin C, Andersson L, Lindhe A. Bone augmentation at fenestrated implants by an osteopromotive membrane technique. : A controlled clinical study. Clin Oral Impl Res 1991;2:159-165 https://doi.org/10.1034/j.1600-0501.1991.020401.x
  3. Becker W, Dahlin C, Becker BE. The use of e-PTFE barrier membranes for bone promotion around titanium implants placed into extraction socket: A prospective multicenter study. Int J Oral Maxillofac Implants 1994;9:31-40
  4. Siebert J, Nyman S. Localized ridge augmentation in dogs. A pilot study using membranes and hydroxyapatite. J Periodontol 1990;61:157-165 https://doi.org/10.1902/jop.1990.61.3.157
  5. Becker W, Becker B, Handlesman M, Celletti R, Ochsenbein C, Hardwick R, Langer B. Bone formation at dehiscenced dental implant sites treated with implant augmentation material : A pilot study in dogs. Int J Periodont Rest Dent 1990;10:93-101
  6. Werrer K, Gotfredsen K, $Hj{\phi}trting-Hansen$ E, Karring T. Guided tissue regeneration of dental implants placed into extraction sockets. An experimental study in monkeys. Clin Oral Impl Res 1991;2:166-171 https://doi.org/10.1034/j.1600-0501.1991.020402.x
  7. Jovanovic SA, Spiekermann H, Richter EJ. Bone regeneration around titanium implants in dehised defect sites. A clinical study. Int J Oral Maxillofac Implants 1992;7:233-245
  8. Jovanovic SA, Schenk RK, Orsini M, Kenney Eb. Supracrestal bone formation around dental implants: An experimental dog study. Int J Oral Maxillofac Implants 1995;10:23-31
  9. Simion M, Trisi P, Piatelli A. Vertical ridge augmentation using a membrane technique associated with osseointegrated implants. Int J Periodont Rest Dent 1994;14:496-511
  10. Tinti C, Parma-Benfenati S, Polizzi G. Vertical ridge augmentation: what is the limit? Int J Periodont Rest Dent 1996;16:221-229
  11. Donos N, Kostopoulos L, Karring T. Augmentation of the rat jaw with autogeneic cortico-cancellous bone grafts and guided tissue regeneration. Clin Oral Impl Res 2002;13:192-202 https://doi.org/10.1034/j.1600-0501.2002.130210.x
  12. Donos N, Kostopoulos L, Karring T. Alveolar ridge augmentation by combining autogenous mandibular bone grafts and non-resorbable membranes. An experimental study in the rat. Clin Oral Impl Res 2002;13:185-191 https://doi.org/10.1034/j.1600-0501.2002.130209.x
  13. Donos N, Kostopoulos L, Karring T. Augmentation of the mandible with GTR and onlay cortical bone grafting. An experimental study in the rat. Clin Oral Impl Res 2002;13:175-184 https://doi.org/10.1034/j.1600-0501.2002.130208.x
  14. von Arx T, Cochran DL, Hermann JS, Schenk RK, Buser D. Lateral ridge augmentation using different bone fillers and barrier membrane application. A histologic and histomorphometric pilot study in the mandible. Clin Oral Impl Res 2001;12: 260-269 https://doi.org/10.1034/j.1600-0501.2001.012003260.x
  15. Malchiodi L, Scarano A, Quaranta M, Piattelli A. Rigid fixation by means of titanium mesh in edentulous ridge expansion for horizontal ridge augmentation in the maxilla. Int J Oral Maxillofac Implants 1998;13:701-705
  16. Slott C, Lundgren D. Augmentation of calvarial tissue using non- permeable silicone domes and bovine bone mineral. An experimental study in the rat. Clin Oral Impl Res 1999;10:468-476 https://doi.org/10.1034/j.1600-0501.1999.100605.x
  17. Jovanovic SA, Schenk RK, Orsini M, Kenney Eb. Supracrestal bone formation around dental implants : an experimental dog study. Int J Periodont Rest Dent 1995;10:23-31
  18. Jovanovic SA, Nevins M. Bone formation utilizing titanium-reinforced barrier membranes. Int J Periodont Rest Dent 1995;15:57-69
  19. Leckholm U, Becker W, Dahlin C, Becker B, Donath K, Morrison E. The role of early versus late removal of GRAM membrane on bone formation at oral implants placed into immediate extraction sockets. An experimental study in dogs. Clin Oral Impl Res 1993;4:121-129 https://doi.org/10.1034/j.1600-0501.1993.040302.x
  20. Piattelli A, Scarano M, Corigliano Piattelli M. Comparison of bone regeneration with the use of mineralized and demineralized freeze-dried bone allografts: a histologic and histochemical study in man. Biomaterials 1996;17:1127-1131 https://doi.org/10.1016/0142-9612(96)85915-1
  21. Nevins M, Mellonig JT. Enhancement of the damaged edentulous ridge to receive dental implants: a combination of allograft and the GORE-TEX membrane. Int J Perio Rest Dent 1992;12:97-111
  22. Nevins M, Mellonig JT, Clem DSC III, Reiser GM, Buser DA. Implants in regenerated bone: long-term survival. Int J Perio Rest Dent 1998;18:35-45
  23. Haas R, Haiolvogl D, Dortbudal O, Mailath G. Freeze-dried bone for maxillary sinus augmentation in sheep. Part II: biomechanical findings. Clin Oral Impl Res 2002;13:581-586 https://doi.org/10.1034/j.1600-0501.2002.130602.x
  24. Feuille F, Knapp CI, Brunsvold MA, Mellonig JT. Clinical and histologic evaluation of bone-replacement grafts in the treatment of localized alveolar ridge defects. Part I : mineralized freeze-dried bone allograft. Int J Periodont Rest Dent 2003;23:29-35
  25. 최성재, 권영혁, 박준봉. 탈회동결건조골과 합성골이식재가 치조골 결손부 재생과정에 미치는 영향. 대한치주과학회지 1994;24:671-684
  26. Shanman RH. A retrospective study of 237 sites treated consecutively with guided tissue regeneration. Int J Perio Rest Dent 1994;14:293-301
  27. van Steenberghe D, Johansson C, Quirynen M, Molly L, Albrektsson T, Naert I. Bone augmentation by means of a stiff occlusive titanium barrier. A study in rabbits and humans. Clin Oral Impl Res 2003;14:63-71 https://doi.org/10.1034/j.1600-0501.2003.140109.x
  28. 이근혁, 권영혁, 박준봉, 허익. 골유도재생술시 그물형 티타늄막의 천공수가 골형성에 미치는 영향. 대한치주과학회지 2003;34:411-424
  29. Lee YM, Nam SH, Kim TI, Lee SJ, Ku Y, Rhyu IC, Chung CP. Enhanced bone augmentation by controlled release of recombinant human bone morphogenetic protein-2 from bioresorbable membranes. J Periodontol 2003;74:865-872 https://doi.org/10.1902/jop.2003.74.6.865
  30. Urist MR. Bone formation by autoinduction. Science 1965;150:893-899 https://doi.org/10.1126/science.150.3698.893
  31. Buck BE, Malinin TI, Brown MD. Bone transplantation and human imunodeficiency virus. An estimate of risk acquired immunodeficiency syndrome(AIDS). Clin Orthop 1989;240:129-136
  32. Friedlander GE, Mellonig JT, Gray JL, Towle HJ. Comparison of freeze-dried bone allograft and porous hydroxyapatite in human periodontal defects. J Periodontol 1965;60:854-858
  33. Berglundh T, Abrahamsson I, Lang NP, Lindhe J. De novo alveolar bone formation adjacent to endosseous implants. A model study in the dog. Clin Oral Impl Res 2003;14:251-262 https://doi.org/10.1034/j.1600-0501.2003.00972.x
  34. Lundgren AK, Lundgren D, H mmerle CHF, Nyman S, Sennerby L. Influence of decortication of the donor bone on guided bone augmentation. Clin Oral Impl Res 2000;11:99-106 https://doi.org/10.1034/j.1600-0501.2000.00002.x
  35. Schmid J, H mmerle CHF, Olah AJ, Lang NP. Membrane permeability is unnecessary for guided generation of new bone. An experimental study in the rabbit. Clin Oral Impl Res 1994;5:125-130 https://doi.org/10.1034/j.1600-0501.1994.050302.x
  36. Majoub Z, Berengo M, Giardino R, Aldini NN, Cordioli G. Role of Intramarrow penetration in osseous repair : A pilot study in the rabbit calvaria. J Periodontol 1999;70:1501-1510 https://doi.org/10.1902/jop.1999.70.12.1501
  37. Zellin G, Linde A. Effect of different osteopromotive membrane porosities on experimental bone neogenesis in rats. Bioimaterials 1996;17:695-702 https://doi.org/10.1016/0142-9612(96)86739-1
  38. Mellonig JT, Bowers GW, Bright RW, Lawrence JJ. Clinical evaluation of freeze-dried allografts in periodontal osseous defects. J Periodontol 1976;47:125-131 https://doi.org/10.1902/jop.1976.47.3.125
  39. Becker W, Lynch SE, Lekholm U, Becker BE, Caffesse R, Donath K, Sanchez R. A comparision of ePTFE membranes alone or in combination with platelet-derived growth factors and insulin-like growth factor-I or demineralized freeze-dried bone in promoting bone formation around immediate extraction socket implants. J. Periodontol 1992;63:929-940 https://doi.org/10.1902/jop.1992.63.11.929
  40. Becker W, Becker BE, Caffesse R. A comparison of demineralized freeze-dried bone and autologous bone to induce bone formation in human extraction sockets. J periodontol 1994;65:1128-1133 https://doi.org/10.1902/jop.1994.65.12.1128
  41. Becker W, Urist MR, Tucker LM, Becker BE, Ochsenbein C. Human demineralized freeze-dried bone : Inadequate induced bone formation in athymic mice. A prliminary report. J Periodontol 1995;66: 822-828 https://doi.org/10.1902/jop.1995.66.9.822
  42. Becker W, Schenk R, Higuchi K, Lekholm U, Becker BE, Variations in bone rgeneration adjacent to implants augmented with barrier membranes alone or with demineralized freeze-dried bone or autologous grafts : A study in dogs. Int J Oral Maxillofac implants 1995;10:143-154
  43. Becker W, Urist M, Becker BE, Jackson W, Parry DA, Bartold M, Vincenzzi G, De Georges D, Niederwanger M. Clinical and histologic observations of sites implanted with intraoral autologous bone grafts or allografts. 15 human case reports. J Periodontol 1996;67:1025-1033 https://doi.org/10.1902/jop.1996.67.10.1025
  44. Nevins M, Mellonig JT. Enhancement of the damaged edentulous ridge to receive dental implants. A combination of allograft and the Gore-Tex membrane. Int J Periodont Rest Dent 1992;12:97
  45. Simion M, Dahlin C, Trisi P, Piatelli A. Qualitative and quantitative comparative study on different filling materials used in bone tissue regeneration : A controlled clinical study. Int J Periodont Rest Dent 1994;14:198-215
  46. Simion M, Trisi P, Piatelli A. GBR with an e-PTFE membrane associated with DFDBA : Histologic and histochemical analysis in a human implant retrieved after 4 years of loading. Int J Periodont Rest Dent 1996;16:338-347
  47. Wlodarski KH, Brodzikowska A. Ability of commercial demineralized freeze-dried bone allograft to induce new bone formation. J periodontol 1996;67:918-926 https://doi.org/10.1902/jop.1996.67.9.918
  48. Wetzel AC, Stich H, Caffesse RG. Bone apposition onto oral implants in the sinus area filled with different grafting materials. A histologic study in beagle dogs. Clin Oral Impl Res 1995;6:155-163 https://doi.org/10.1034/j.1600-0501.1995.060304.x
  49. Sigurdsson TJ, Nygaard L, Tatakis DN, Fu E, Turek TJ, Jin L, Wozney JM, Wikesjo UME. Periodontal repair in dogs: Evaluation of rhBMP-2 carriers. Int J Periodont Rest Dent 1996;16:525-537
  50. Stavropoulos. A., Kostopoulos, L., Mardas, N., Nyengaard. J. R. & Karring, T. Deproteinized bovine bone used as an adjunct to guided bone augmentation (GBA). An experimental study in the rat. Clinical Implant Dentistry and Related Research 2001;3:156-165 https://doi.org/10.1111/j.1708-8208.2001.tb00136.x
  51. Stavropoulos. A., Kostopoulos, L., Mardas, N., Nyengaard, J. R. & Karring, T. Deproteinized bovine bone(Bio-Oss) and bioactive glass(Biogran) arrest bone formation when used as an adjunct to guided tissue regeneration(GTR). An experimental study in the rat. J Clin Periodontol 2003;30:636-643 https://doi.org/10.1034/j.1600-051X.2003.00093.x
  52. 이진, 권영혁, 박준봉, 허익, 정종혁, 김종관. 티타늄 강화 차폐막의 골유도 재생 효과. 대한치주과학회지 2004;34:711-721
  53. 홍소미, 허익, 권영혁, 박준봉. 동결건조골이 재생공간 유지 및 골재생에 미치는 영향. 대한치주과학회지 2004;34:149-161