Journal of the Korean Society for Precision Engineering (한국정밀공학회지)

Korean Society for Precision Engineering (한국정밀공학회)
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Nano-Bio Applications Using Carbon Nanotube-Biomolecule Conjugates

탄소 나노튜브와 생체 분자와의 결합을 통한 나노-바이오 응용

  • 황응수 (서울대학교 의과대학) ;
  • 조승범 (성균관대학교 기계공학과) ;
  • 홍상현 (성균관대학교 기계공학과) ;
  • 정혜진 (서울대학교 의과대학) ;
  • 차창용 (서울대학교 의과대학) ;
  • 최재붕 (성균관대학교 기계공학과) ;
  • 김영진 (성균관대학교 기계공학과) ;
  • 백승현 (성균관대학교 기계공학과)
  • Published : 2006.03.01
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Abstract

Single-walled carbon nanotubes (SWNT) exhibit strong Raman signals as well as fluorescence emissions in the near infrared regions where most biomolecules are transparent. Such signals do not blink or photobleach under prolonged excitation. which is advantageous to optical nano-bio marker applications. In this paper, single walled carbon nanotubes are conjugated with specific types of single-stranded DNA in order to detect oligonucleotides of corresponding complimentary sequences. Dot blotting experiments and comparative Raman spectroscopy observations demonstrated excellent sensitivity and specificity of carbon nanotube-DNA probes. The results show the possibility of using SWNT as generic nano-bio markers for the precise detection of specific kinds of genes.

Keywords

References

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