Korean journal of food and cookery science (한국식품조리과학회지)

Korean Society of Food and Cookery Science (한국식품조리과학회)
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Functional Properties of Medicinal Plant Extracts

한방식재료 추출물의 기능성

  • Park, Chan-Sung (Dept. of Herbal Food Couisine & Nutrition, Daegu Haany University) ;
  • Yang, Kyung-Mi (Dept. of Herbal Food Couisine & Nutrition, Daegu Haany University) ;
  • Kim, Mi-Lim (Dept. of Herbal Food Couisine & Nutrition, Daegu Haany University)
  • 박찬성 (대구한의대학교 한방식품조리영양학부) ;
  • 양경미 (대구한의대학교 한방식품조리영양학부) ;
  • 김미림 (대구한의대학교 한방식품조리영양학부)
  • Published : 2006.10.31
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Abstract

The purpose of this study was to investigate the functional properties of medicinal plant extracts. Four kinds of medicinal plants, Dioscorea batatas(DB), Armeniacae Semen(AS) Crataegus pinnatifida Bunge(CP) and Ponciri Fractus(PF), were extracted with water and 70% ethanol and the extracts were tested for their electron donating ability(EDA), nitrite scavenging ability(NSA) and inhibitory effects on cancer cells(MDA cell and A549 cell)growth. EDA at 100-1,000 ppm of water extract ranged from 3% to 14%, 14% to 36%, 29% to 72% and 14% to 43%, and that of ethanol extract ranged from 9% to 62%, 27% to 59%, 33% to 89% and 14% to 44%, in DB, AS, CP and PF, respectively. NSA of extracts measured at various pH(1.2, 3.0, 4.2, 6.0) showed the highest ability in all extracts at pH 1.2 and decreased with increasing pH. The highest NSA of water extracts of 1,000 ppm at pH 1.2 was 6%, 31%, 55% and 44% and that of ethanol extract was15%, 32%, 69% and 52%, in DB, AS, CP and PF, respectively Inhibition ratio of water and ethanol extracts on MDA cell growth was 24% and 17%, 51% and 93%, 46% and 69%, and 48% and 47%, while that on A549 cell was 18% and 9%, 6% and 3%, 7% and 3%, and 43% and 11%, at 1,000 ppm, in DB, AS, CP and PF, respectively.

본 연구는 산약, 행인, 산사자, 지실 등 4종류의 한약재를 물과 에탄올로 추출하여 전자공여능, 아질산염소거능 및 암세포 증식억제능을 검토하여 이들을 이용한 기능성 식품제조에 필요한 기초자료를 얻고자 하였다. 전자공여능은 물 추출물 1,000 ppm에서 산약은 14%, 행인은 36%, 산사자는 72% 그리고 지실은 43%였고, 에탄올 추출물의 전자공여능은 1,000 ppm에서 산약은 62%, 행인은 59%, 산사자는 89% 그리고 지실은 42%였다. 4가지 약재 모두에서 물 및 에탄올 추출물 농도가 올라 갈수록 전자공여능도 높아져 1,000 ppm에서 가장 높았고 1,000 ppm에서 물 추출물은 산사자, 지실, 행인 그리고 산약의 순서였으며 에탄올 추출물은 산사자, 행인, 지실 그리고 산약의 순서로 산사자의 전자공여능이 가장 높았고 산약이 가장 낮았다. 산약, 행인, 산사자 및 지실의 아질산염 소거능은 물 및 에탄올 추출물 모두에서 pH가 낮아짐에 따라 아질산염 소거능은 증가하여 pH 1.2에서 가장 높았고 동일한 pH에서는 농도 의존적으로 아질산염 소거능도 증가하였다. pH 1.2에서 물 추출물 1,000 ppm의 아질산염 소거능은 산약이 5%, 행인이 31%, 산사자가 55% 그리고 지실이 45%였고 동일농도에서 에탄올 추출물은 산약이 18%, 행인이 32%, 산사자가 69% 그리고 지실이 52%였다. 물 및 에탄올 추출물 모두에서 산사자 가 가장 높은 아질산염 소거능을 보였고 지실, 행인 그리고 산약의 순서였다. 물 추출물과 에탄올 추출물의 MDA-cell(유방암 세포)에 대한 증식억제능으로 행인과 산사자는 물 추출물보다 에탄올 추출물이 모든 농도에서 유의적으로 높았고 산약과 지실은 에탄을 추출물보다 물 추출물이 높았으나 유의적인 차이는 없었다. 특히 에탄올 추출물 1,000 ppm에서 행인과 산사자의 MDA cell에 대한 증식억제능은 94% 및 69%로 매우 높았다. 물 추출물과 에탄올 추출물의 A549 cell(폐암세포)에 대한 증식억제능은 물 및 에탄올 추출물 모두에서 지실이 가장 높아 물 추출물 1,000 ppm에서 45%였다.

Keywords

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