Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Increase of Membrane Potential by Ginsenosides in Prostate Cancer and Glioma cells

  • Lee, Yun-Kyung (Laboratory of Pharmacology, College of Pharmacy and Research Institute for Drug Development, Pusan National University) ;
  • Im, Young-Jin (Laboratory of Pharmacology, College of Pharmacy and Research Institute for Drug Development, Pusan National University) ;
  • Kim, Yu-Lee (Laboratory of Pharmacology, College of Pharmacy and Research Institute for Drug Development, Pusan National University) ;
  • Sacket Santosh J. (Laboratory of Pharmacology, College of Pharmacy and Research Institute for Drug Development, Pusan National University) ;
  • Lim, Sung-Mee (Laboratory of Pharmacology, College of Pharmacy and Research Institute for Drug Development, Pusan National University) ;
  • Kim, Kye-Ok (Laboratory of Pharmacology, College of Pharmacy and Research Institute for Drug Development, Pusan National University) ;
  • Kim, Hyo-Lim (Laboratory of Pharmacology, College of Pharmacy and Research Institute for Drug Development, Pusan National University) ;
  • Ko, Sung-Ryong (KT&G Central Research Institute) ;
  • Lm, Dong-Soon (Laboratory of Pharmacology, College of Pharmacy and Research Institute for Drug Development, Pusan National University)
  • Published : 2006.06.01
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Abstract

Ginseng has an anti-cancer effect in several cancer models. As a mechanism study of ginsenoside-induced growth inhibition in cancer cells, we measured change of membrane potential in prostate cancer and glioma cells by ginsenosides, active constituents of ginseng. Membrane potential was estimated by measuring fluorescence change of DiBAC-Ioaded cells. Among 11 ginsenosides tested, ginsenosides $Rb_2$, $Rg_3$, and $Rh_2$ increased significantly and robustly the membrane potential in a concentration-dependent manner in prostate cancer and glioma cells. Ginsenosides Rc, Ro, and $Rb_1$ slightly increased membrane potential. The ginsenoside-induced membrane potential increase was not affected by treatment with pertussis toxin or U73122. The ginsenoside-induced membrane potential increase was not diminished in $Na^+$-free or $HCO_3^-$-free media. Furthermore, the ginsenoside-induced increase of membrane potential was not changed by EIPA (5-(N-ethyl-N-isopropyl)-amiloride), SITS (4-acetoamido-4'-isothiocyanostilbene-2,2'-disulfonic acid), and omeprazole. In summary, ginsenosides $Rb_2$, $Rg_3$, and $Rh_2$ increased membrane potential in prostate cancer and glioma cells in a GPCR-independent and $Na^+$ independent manner.

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References

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