Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

The Antioxidative and Antimicrobial Effects of Gloiopeltis Tenax

참가사리 분획물의 항산화.항균효과

  • Jung Young-Hwa (Department of Food and nutrition, Marine biotechnology Center for Biofunctional Material Industries, Silla University) ;
  • Jung Bok-Mi (Department of Food Science and Nutrition, Yosu National University) ;
  • Kang Dae-Yeon (Department of Food and nutrition, Marine biotechnology Center for Biofunctional Material Industries, Silla University) ;
  • Ku Mi-Jeong (Department of Food and nutrition, Marine biotechnology Center for Biofunctional Material Industries, Silla University) ;
  • Shin Mi-Ok (Department of Food and nutrition, Marine biotechnology Center for Biofunctional Material Industries, Silla University) ;
  • Bae Song-Ja (Department of Food and nutrition, Marine biotechnology Center for Biofunctional Material Industries, Silla University)
  • 정영화 (신라대학교 식품영양학과, 마린-바이오 산업화지원센터) ;
  • 정복미 (여수대학교 식품영양학과) ;
  • 강대연 (신라대학교 식품영양학과, 마린-바이오 산업화지원센터) ;
  • 구미정 (신라대학교 식품영양학과, 마린-바이오 산업화지원센터) ;
  • 신미옥 (신라대학교 식품영양학과, 마린-바이오 산업화지원센터) ;
  • 배송자 (신라대학교 식품영양학과, 마린-바이오 산업화지원센터)
  • Published : 2006.06.01
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Abstract

In this study, we investigated the antioxidative and antimicrobial activities of red algae Gloiopeltis tenax (GT). GT was extracted with methanol and then further fractionated it into four different types: methanol (GTMM), hexane (GTMH), butanol (GTMB) and aqueous (GTMA) soluble fractions. The antioxidant activity of the fractions from GT was investigated by measuring the scavenging activities of GT against reactive oxygen species (ROS) and reactive nitrogen species (RNS). Among the four fractions of GT, GTMM and GTMB showed a marked scavenging effect against ROS, but they displayed very low levels of the scavenging effect against RNS. The antimicrobial activity was increased in proportion to its concentration by the paper disc method. Among the various solvent layers, the GTMM and GTMB showed strong antimicrobial activities.

참가사리의 항산화효과를 측정하기 위해 ROS와 RNS 제거활성을 측정하였다. ROS 제거활성 측정결과 GTMM층과 GTMB층이 positive control로 사용한 trolox와 유사한 높은 제거능을 보였고, $ONOO^-$ 제거능은 그 효과가 미약하였으나 GTMM 층에서 나타났으며 NO 제거활성 역서 GTMM층과 GTMB층에서 약한 효과가 관찰되었다. 따라서 본 실험의 결과 GTMM 층에서 ROS 제거 활성의 효과가 높았으며 다음으로 GTMB층에서 효과를 보여 GTMM층과 GTMB층에 활성산소 제거효과를 가지는 물질이 있는 것으로 사료되어진다. Paper disc method를 이용한 항균활성 효과 실험결과, Serratia marcescens가 모든 층에서 높은 항균력을 보였으며 Proteus mirabilis에서도 GTMA층을 제외한 모든 층에서 항균효과를 보였다. 사용한 모든 균주에서 각각 항균력의 차이는 있으나 전체적으로 GTMB 층과 GTMM 층에서 높은 항균활성을 관찰할 수 있었다. 이 연구결과를 토대로 참가사리의 항산화 및 항균활성물질에 대한 단계적인 분리 동정이 이루어져 GTMM 층과 GTMB 층의 생리활성물질 개발이 기대되는 바이다.

Keywords

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