Determination and Generation Factor of Acrylamide Content in the French Fries and Snacks by HPLC/MS/MS

HPLC/MS/MS를 이용한 French Fries와 스낵류에서의 Acrylamide 함량 분석 및 생성요인

  • Park Geon-Yong (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee Sung-Deuk (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Chang Min-Su (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Choi Young-Hee (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim Eun-Hee (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Han Sang-Un (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Chough Nam-Joon (Seoul Metropolitan Government Research Institute of Public Health and Environment)
  • 박건용 (서울특별시보건환경연구원) ;
  • 이성득 (서울특별시보건환경연구원) ;
  • 장민수 (서울특별시보건환경연구원) ;
  • 최영희 (서울특별시보건환경연구원) ;
  • 김은희 (서울특별시보건환경연구원) ;
  • 한상운 (서울특별시보건환경연구원) ;
  • 조남준 (서울특별시보건환경연구원)
  • Published : 2006.06.01

Abstract

The formed acrylamide in many fried and baked starchy foods is in consequence of its formation during frying and preparation methods. Being acrylamide established to be a toxic substance, the implications to public health from amounts found in food are not clear. So this study was carried out to investigate the contents of acrylamide of French fries and snacks in addition to acrylamide formation in relation to water and lipid in foods. The raw materials of foods used in the experiments were wheat, potato, com and rice. The preparation of foods was modified in a little to raise the recovery ratio and contents of water and lipid were analyzed to the public food method. The contents of acrylamide were measured by the liquid chromatography tandem mass spectrometry. The average level of acrylamide produced in snacks is $236{\pm}322$ ppb and potato snacks came out the highest value at $521{\pm}403$ ppb in 104 snacks. The formation of acrylamide in fried food was found to depends on the composition of raw material not on water and lipid contents in food and not on storage period and temperature.

유통 중인 스낵과자 104건에서 acrylamide함량조사 결과는 $236{\pm}322$ ppb로 측정되었으며 제품마다 차이가 크게 나타났다. 스낵과자는 그 원료에 따라 함량에 큰 차이를 나타내어 감자, 밀, 옥수수 순으로 측정되었고 감자스낵을 대상으로 국내산과 수입산의 함량을 조사한 결과 국내산이 약간 높게 측정되었다. 또한 패스트푸드점의 french fries 30건을 대상으로 측정한 acrylamide함량조사 결과는 $378{\pm}221$ ppb이었으며 제조회사별로 함량 차이가 크게 나타났다. French fries의 수분의 양과 acrylamide의 상관계수는 0.55이었고,지방 함량과의 상관계수는 0.25이었다. 제품의 저장에 따른 변화는 크지 않았으며 생산과정에서의 적절한 제조조건의 설정이 acrylamide 저감에 중요함을 알 수 있었다.

Keywords

References

  1. Ehling, S. and Shibamoto, T.: Correlation of Acrylamide generation in thermally processed model system of asparagine and glucose with color formation, amounts of pyrazines formed, and antioxidative properties of extracts, Agricultural and food chemistry, 53(4), 813-4819(2005)
  2. Knol, J.K., van Loon, A.M., Linssen, P.H., Ruck, A.L., van Boekel, AJ.S. and Voragen, GJ.: Toward a kinetic model for ACrylamide formation in a glucose-asparagine reaction system, Agricultural and food chemistry, 53(6), 133-6139 (2005)
  3. Jung, M.Y., Choi, D.S. and Ju, J.w.: A noval technique for limitation of ACrylamide formation in fried and baked com chips and in french fries, Journal of food science, 68, 1287-1290(2004) https://doi.org/10.1111/j.1365-2621.2003.tb09641.x
  4. Zyzak, D.V., Sandera, R.A., Stojanovic, M., Tallmadge, D.H., Eberhart, B.L., Ewald, D.K., Gruber, D.C., Morsch, T.R., Strothers, M.A., Rizzi, GP. and Villagran: Acrylamide formation mechanism in heated foods. Agricultural andfood chemistry, 51, 4782-4787(2003) https://doi.org/10.1021/jf034180i
  5. Erland, B., Agnieszka, K., Svein, H. and Trude, w.: Addition of glycine reduces the content of Acrylamide in cereal and potato products. Agricultural and food chemistry, 53, 3259-3264(2005) https://doi.org/10.1021/jf048082o
  6. Kim, C.T., Hwang, E.S. and Lee, HJ.: Reducing Acrylamide in fried snack products by adding amino acids. Food chemistry and toxicology, 70, C334-C358(2005)
  7. Granda, C., Moreira, R.G. and Tichy, S.E.: Reduction of Acrylamide formation in potato chips by low-temperature vacuum flying, Journal of food science, 69, E405-E411 (2004) https://doi.org/10.1111/j.1365-2621.2004.tb09903.x
  8. Grob, K.: Reduction of exposure to Acrylamide, Journal of AOAC international, 88, 253-261(2005)
  9. Kita, A., Brathen, E., Knutsen, S.H. and Wicklund, T.: Effective ways of decreasing acrylamide content in potato crips during processing. Agricultural and food chemistry, 52, 7011-7016(2004) https://doi.org/10.1021/jf049269i
  10. Siayne, M.A.: acrylamide:Consoderations for risk management, Journal of AOAC international, 88, 227-233(2005)
  11. Tareke, E., Rydberg, P., Karlsson, P., Eriksson, S. and Tornqvist, M.: Analysis of acrylamide, a carcinogen formed in heated foodstuffs. Agricultural and food chemistry, 50, 4998 - 5006(2002) https://doi.org/10.1021/jf020302f
  12. Rosen, J. and Hellenas, K.E.: Analysis of acrylamide in cooked foods by liquid chromatography tandem mass spectrometry. Analyst, 127, 880-882(2002) https://doi.org/10.1039/b204938d
  13. Owen, L.M., Castle, L., Kelly, J., Wilson, L. and Lloyd, A.S. : acrylamide analysis: Assessment of results from six rounds of food analysis performance assessment scheme proficiency testing. Journal of AOAC international, 88, 285-291(2005)
  14. Maiyani, T. : acrylamide in foods.食衛諒, 43, J-348- 351(2002)
  15. Takatsuki,S.,Nemoto,S.,Sasaki,K. and Maitani,T.: Determinationofacrylamideinprocessedfoodsby LC/MS usingcolumnswitching 食筋諒, 44, 89-95(2003)
  16. Lineback,D.:Overview of acrylamide monitoring databases. Journal of AOAC international,88,246-252(2005)
  17. Hoenicke,K. and Gatermann,R.: Studiesonthe stability of acrylamide in food during storage. Journal of AOAC international,88,268-272(2005)