Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Isolation of SYP61/OSMl that is Required for Salt Tolerance in Arabidopsis by T-DNA Tagging

애기장대에서 고염 스트레스 내성에 관여하는 OSM1/SYP61 유전자의 동정

  • Kim, Ji-Yeon (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity) ;
  • Baek, Dong-Won (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity) ;
  • Lee, Hyo-Jung (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity) ;
  • Shin, Dong-Jin (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity) ;
  • Lee, Ji-Young (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity) ;
  • Choi, Won-Kyun (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity) ;
  • Kim, Dong-Giun (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity) ;
  • Chung, Woo-Sik (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity) ;
  • Kwak, Sang-Soo (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Yun, Dae-Jin (Division of Applied Life Science (BK21 program), and Environmental Biotechnology National Core Research Center, Gyeongsang National Univerity)
  • 김지연 (경상대학교 대학원 응용생명과학부) ;
  • 백동원 (경상대학교 대학원 응용생명과학부) ;
  • 이효정 (경상대학교 대학원 응용생명과학부) ;
  • 신동진 (경상대학교 대학원 응용생명과학부) ;
  • 이지영 (경상대학교 대학원 응용생명과학부) ;
  • 최원균 (경상대학교 대학원 응용생명과학부) ;
  • 김동균 (경상대학교 대학원 응용생명과학부) ;
  • 정우식 (경상대학교 대학원 응용생명과학부) ;
  • 곽상수 (한국생명공학연구원 환경생명공학연구센터) ;
  • 윤대진 (경상대학교 대학원 응용생명과학부)
  • Published : 2006.03.01
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Abstract

Salt stress is one of major environmental factors influencing plant growth and development. To identify salt tolerance determinants in higher plants, a large-scale screen was conducted with a bialaphos marker-based T-DNA insertional collection of Arabidopsis ecotype C24 mutants. One line for salt stress-sensitive mutant (referred to as ssm1) exhibited increased sensitivity to both ionic (NaCl) and nonionic (mannitol) osmotic stress in a root growth assay. This result suggests that ssm1 mutant is involved in ion homeostasis and osmotic compensation in plant. Molecular cloning of the genomic DNA flanking T-DNA insert of ssm1 mutant was achieved by mutant genomic DNA library screening. T-DNA insertion appeared in the first exon of an open reading frame on F3M18.7, which is the same as AtSYP61. SSM1 is SYP61/OSM1 that is a member of the SNARE superfamily of proteins required for vesicular/target membrane fusions and factor related to abiotic stress.

고염 스트레스에 대한 연구는 농업 생산성에 직결되기 때문에, 고염에 대한 식물의 반응 및 신호전달, 적응기작은 중요한 연구주제가 되어 왔다. 현재까지 연구된 고염 스트레스에 대한 저항성 기작 및 유전학적 요소들이 많이 밝혀졌는데도 불구하고 아직 많은 연구를 필요로 하고 있다. 그래서 본 연구에서는 모델식물로 잘 알려진 애기장대에 pSK1015 vector로 T-DNA를 삽입하여 고염 스트레스에 대해 감수성을 보이는 돌연변이체, ssm1 돌연변이체를 선별하였다. ssm1 돌연변이체는 고염 스트레스를 받게 되면 이온의 독성 스트레스와 세포내 삼투압의 불균형에서 오는 스트레스에 대해 대조군에 비해 감수성을 보였다. ssm1 돌연변이체의 genomic DNA 상의 T-DNA가 삽입된 부위를 찾기 위하여 genomic DNA mutant library screening을 수행한 결과, 기존의 알려진 syntaxin 기능 및 환경 스트레스에 관련된 F3M18/AtSYP61/OSM1 임을 알 수 있었다.

Keywords

References

  1. Baek D, Nam J, Koo YD, Kim DH, Lee J, Jeong JC, Kwak SS, Chung WS, Lim CO, Bahk JD, Hong JC, Lee SY, Kawai-Yamada M, Uchimiya H, Yun DJ. (2004) Baxinduced cell death of Arabidopsis is meditated through reactive oxygen-dependent and -independent processes. Plant Mol Biol. 56: 15-27 https://doi.org/10.1007/s11103-004-3096-4
  2. Bezprozvanny I, Zhong P, Scheller RH, Tsien RW (2000) Molecular determinants of the functional interaction between syntaxin and N-type $Ca^{2+}$ channel gating. Proc Natl Acad Sci USA 97: 13943-13948
  3. Delauney AJ, Verma DPS (1993) Proline biosynthesis and osmoregulation in plants. Plant J 4: 215-223 https://doi.org/10.1046/j.1365-313X.1993.04020215.x
  4. Grillo S, Leone A, Xu Y, Tucci M, Francione R, Hasegawa PM, Monti L, Bressan RA (1995) Control of osmotin gene expression by ABA and osmotic stress in vegetative tissues of wild-type and ABA-deficient mutants of tomato. Physiol Plant 93: 498-504 https://doi.org/10.1111/j.1399-3054.1995.tb06849.x
  5. Knight H, Trewavas AJ, Knight MR (1997) Calcium signaling in Arabidopsis thaliana responding to drought and salinity. Plant J 12: 1067-1078 https://doi.org/10.1046/j.1365-313X.1997.12051067.x
  6. Louis P, Galinski EA (1997) Characterization of genes for the biosynthesis of the compatible solute ectoine from Marinococcus halophilus and osmoregulated expression in E. coli. Microbiol. 143: 1141-1151 https://doi.org/10.1099/00221287-143-4-1141
  7. McCue KF, Hanson AD (1990) Drought and salt tolerance: Towards understanding and application. Biotechnol 8: 358-362 https://doi.org/10.1038/nbt0490-358
  8. Qi Z, Spalding EP (2004) Protection of plasma membrane $K^+$transport by the salt overly sensitive 1 $Na^+/H^+$ antiporter during salinity stress. Plant Physiol 136: 2548-2555 https://doi.org/10.1104/pp.104.049213
  9. Qui QS, Guo Y, Dietrich MA, Schumaker KS, Zhu JK (2002) Regulation of SOS1, a plasma membrane $Na^+/H^+$> exchanger in Arabidopsis thaliana, by SOS2 and SOS3. Proc Natl Acad Sci USA 99: 8436-8441
  10. Qiu QS, Guo Y, Quintero FJ, Pardo JM, Schumaker KS, Zhu JK (2004) Regulation of vacuolar $Na^+/H^+$ exchange in Arabidopsis thaliana by the salt-overly-sensitive (SOS) pathway. J Biol Chem 279: 207-215 https://doi.org/10.1074/jbc.M307982200
  11. Quintero FJ, Ohta M, Shi H, Zhu JK, Pardo JM (2002) Reconstitution in yeast of the Arabidopsis SOS signaling pathway for $Na^+$ homeostasis. Proc Natl Acad Sci USA 99: 9061-9066
  12. Rus A, Lee BH, Munoz-Mayor A, Sharkhuu A, Miura K, Zhu JK, Bressan RA, Hasegawa PM (2004) AtHKTl facilitates $Na^+$ homeostasis and $K^+$ nutrition in planta. Plant Physiol 136: 2500-2525 https://doi.org/10.1104/pp.104.042234
  13. Rus A, Yokoi S, Sharkhuu A, Reddy M, Lee BH, Matsumoto TK, Koiwa H, Zhu JK, Bressan RA, Hasegawa PM (2001) AtHK1 is a salt tolerance determinant that controls $Na^+$ entry into plant roots. Proc Natl Acad Sci USA 98: 14150-14155
  14. Sanderfoot AA, Assaad FF, Raikhel NV (2000) The Arabidopsis genome; An abundance of soluble N-ethylmaleimidesensitive factor adaptor protein receptors. Plant Physiol 124: 1558-1569 https://doi.org/10.1104/pp.124.4.1558
  15. Shi H, Lee BH, Wu SJ, Zhu JK (2003) Overexpression of a plasma membrane $Na^+/H^+$ anti porter gene improves salt tolerance in Arabidopsis thaliana. Nature Biotechnol 21: 81-85 https://doi.org/10.1038/nbt766
  16. Shinozaki K, Yamaguchi-Shinozaki K (1997) Gene expression and signal transduction in water-stress response. Plant Physiol 115: 327-334 https://doi.org/10.1104/pp.115.2.327
  17. Weigel D, Ahn JH, Blazquez MA, Borevitz JO, Christensen SK, Fankhauser C, Ferrandiz C, Kardailsky I, Malancharuvil EJ, Neff MM, Nguyen JT, Sato S, Wang ZY, Xia Y, Dixon RA, Harrison MJ, Lamb CJ, Yanofsky MF, Chory J. (2000) Activation Tagging in Arabidopsis. Plant Physiol 122: 1003-1014 https://doi.org/10.1104/pp.122.4.1003
  18. Yokoi S, Quintero FJ, Cubero B, Ruiz MT, Bressan RA, Hasegawa PM, Pardo JM (2002) Differential expression and function of Arabidopsis thaliana NHX $Na^+/H^+$ antiporters in the salt stress response. Plant J 30: 529-539 https://doi.org/10.1046/j.1365-313X.2002.01309.x
  19. Yun DJ (2005) Molecular mechanism of plant adaption to high salinity. Korean J Plant Biotechnol 32: 1-14 https://doi.org/10.5010/JPB.2005.32.1.001
  20. Zhu J, Gong Z, Zhang C, Song AP, Damsz B, Inan G, Koiwa H, Zhu JK, Hasegawa PM, Bressan RA (2002) OSM1/ SYP61: A syntaxin protein in Arabidopsis controls abscisic acid-mediated and non-absclsic acid-mediated responses to abiotic stress. Plant Cell 14: 3009-3028 https://doi.org/10.1105/tpc.006981
  21. Zhu JK (2001) Plant salt tolerance. Trends Plant Sci 6: 66-71 https://doi.org/10.1016/S1360-1385(00)01838-0
  22. Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53: 247-273 https://doi.org/10.1146/annurev.arplant.53.091401.143329
  23. Zhu JK (2003) Regulation of ion homeostasis under salt stress. Curr Opin Plant Biol 6: 441-445 https://doi.org/10.1016/S1369-5266(03)00085-2