Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Inhibition of Proliferation and Induction of Apoptosis by EGCG in Human Osteogenic Sarcoma (HOS) Cells

  • Ji Sang-Jin (Department of Biochemistry, College of Dentistry, KyungHee University) ;
  • Han Dong-Hoon (Department of Biomedical Science, KyungHee University) ;
  • Kim Jeong-Hee (Department of Biochemistry, College of Dentistry, KyungHee University)
  • Published : 2006.05.01
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Abstract

EGCG [(-)-epigallocatechin-3-gallate], a major component of green tea has been considered as a major antioxidant constituent. In addition to having been considered for cancer treatment as a chemopreventive and chemotherapeutic agent, EGCG has recently been attributed an anti-proliferative effect. We re-examined the latter finding in this study and added specific focus on the ability of EGCG to induce apoptosis in human osteogenic sarcoma (HOS) cells. Antiproliferative action of EGCG $(IC_{50}=35.3{\pm}6.0{\mu}g/mL)$ appeared to be linked to apoptotic cell death based on morphological changes, chromosomal DNA degradation, and an increase in the $sub-G_1$ apoptotic cell population. Treatment of HOS cells with EGCG gradually activated caspase-3, an established inducer of apoptotic cell death.

Keywords

References

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