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Biological Control of Sclerotinia sclerotiorum Using Indigenous Chitinolytic Actinomycetes in Jordan

  • Tahtamouni M.E.W. (Department of Plant Production, Jordan University of Science and Technology) ;
  • Hameed K.M. (Department of Plant Production, Jordan University of Science and Technology) ;
  • Saadoun I.M. (Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology)
  • 발행 : 2006.06.01

초록

The white cottony stem rot pathogen Sclerotinia scierotiorum was subjected to 70 different isolates of actinomycetes indigenous to Jordan as biological control agents. Forty of them demonstrated chitinase activity on crab shell chitin agay (CCA) media and they were segregated into three groups: 14 highly active, 12 moderately active, and 14 with low activity, with average clearing zones of (4.7-8.3), (3.7-4.3), and (2.3-3.3) mm surrounding colonies on CCA, respectively. Further, these isolates were able to inhibit radial mycelium growth of the pathogen and were categorized into three antagonistic groups: 13 strong, 13 moderate, and 14 weak antagonists, with antibiosis inhibition Bones of (32.0-45.7), (22.7-31.3), and (3.7-22.3) mm, respectively. High levels of chitinase activity of the isolates Ma3 (8.3 mm), Jul (7.7 mm), and Sa8 (7.7 mm) with their antagonistic activity against mycelium growth of 45.7, 44.3, and 40.7 mm were observed, respectively. These isolates exhibited fungicidal activity against sclevotia of S. sclerotiorum. On the other hand, isolates Na5, Aj3, and Aj2 that produced no chitinase showed fungistatic effect only.

키워드

참고문헌

  1. Adams, P. and Ayers, W. 1979. Ecology of Sclerotinia species. Phytopathology 69:895-898
  2. Boland, G and Smith, E. 1991. Variation in cultural morphology and virulence among protoplast regenerated isolates of Sclerotinia sclerotiorum. Phytopathology 81:766-770 https://doi.org/10.1094/Phyto-81-766
  3. EI-Tarabily, K., Soliman, M., Nassar, A., AI-Hassani, H., Sivasithamparam, K., McKenna, F. and Hardy, GE StJ. 2000. Biological control of Sclerotinia minor using a chitinolytic bacterium and actinomycetes. Plant Pathol. 49:573-583 https://doi.org/10.1046/j.1365-3059.2000.00494.x
  4. Freeman, J., Ward, E., Carmen, C. and Alastair, M. 2002. A polymerase chain reaction (PCR) assay for the detection of inoculum of Sclerotinia sclerotiorum. Eur. J. Plant Pathol. 108:877-886 https://doi.org/10.1023/A:1021216720024
  5. Gupta, R., Saxena, R., Chaturvedi, P. and Virdi, J. 1995. Chitinase production by Streptomyces viridificans: its potential in fungal cell wall lysis. J. Appl. Bact. 78:378-383 https://doi.org/10.1111/j.1365-2672.1995.tb03421.x
  6. Hus, S. and Lockwood, J. 1975. Powder chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl. Microbiol. 29 :422-426
  7. Lumsden, R. 1979. Histology and physiology of pathogenesis in plant diseases caused by Sclerotinia spp. Phytopathology, 69:890-895 https://doi.org/10.1094/Phyto-69-890
  8. Ordentlich, A., Elad, Y.a nd Chet, I. 1988. The role of chitinase of Serratia marcescens in biological control of Sclerotium rolfsii. Phytopathology 78:84-88
  9. Osofee, H., Hameed, K. and Mahasneh, A. 2005. Relatedness among indigenous members of Sclerotinia, sclerotiorum by mycelial compatibility and RAPD analysis in the Jordan Valley. Plant Pathol. J. 21:106-107 https://doi.org/10.5423/PPJ.2005.21.2.106
  10. Patterson, C. and Grogan, R. 1985. Differences in epidemiology and control of lettuce drop caused by Sclerotinia minor and Sclerotinia sclerotiorum. Plant Dis. 69:766-770 https://doi.org/10.1094/PD-69-766
  11. Purdy, L. 1979. Sclerotinia sclerotiorum: History, diseases and Symptomology, host range, geographic distribution, and impact. Phytopathology, 69:875-880 https://doi.org/10.1094/Phyto-69-875
  12. Saadoun, I. and AL-Momani, F. 1997. Streptomyces from Jordan soils active against Agrobacterium tumefaciens. Actinomycetes 8:30-36
  13. Saadoun, I. and Gharaibeh, R. 2002. The Streptomyces flora of Jordan and its potential as a source of antibiotics active against antibiotic resistant Gram-negative bacteria. World J. Microbial Biotech.18:465-470 https://doi.org/10.1023/A:1015531205871
  14. Saadoun, I., Hameed, K., AL-Momani, F., Malkawi, H., Meqdam, M. and Mohammad, M. 2000. Characterization and analysis of antifungal activity of soil Streptomycetes isolated from north Jordan. Egypt. J. Microbiol. 35 :463-471
  15. Shirling, E. B. and Gottlieb, D. 1964. Methods for characterization of Streptomyces species. Inter. J. Syst. Bacteriol. 16:313-340
  16. Singh, P., Shin, Y, Park, C. and Chung, Y 1999. Biological control of Fusarium wilt of cucumber by chitinolytic bacteria. Phytopathology, 89:92-99 https://doi.org/10.1094/PHYTO.1999.89.1.92
  17. Subbarao, K. 1998. Progress toward integrated management of lettuce drop. Plant Dis. 82:1068-1078 https://doi.org/10.1094/PDIS.1998.82.10.1068

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