The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Development of Single-tube Multiplex Immunocapture RT-PCR Assay for Simultaneous Detection of Two Pepper Tobamoviruses

  • Kim, Jae-Hyun (Bio-resources, Research Group, KT&G Central Research Institute) ;
  • Choi, Gug-Seoun (National Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Jeong-Soo (National Horticultural Research Institute, Rural Development Administration) ;
  • Lee, Sin-Ho (National Horticultural Research Institute, Rural Development Administration) ;
  • Choi, Jang-Kyung (Division of Biological Environment, Kanwon National University) ;
  • Ryu, Ki-Ryun (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University)
  • Published : 2006.06.01
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Abstract

An immunocapture reverse transcription-polymerase chain reaction (IC/RT-PCR) was developed for simultaneous detection of two pepper-infecting RNA viruses, Pepper mud mottle virus (PMMoV) and Tobacco mild green mosaic virus (TMGMV). The assay could be performed in a single tube for simultaneous and sensitive detection of these tobamoviruses. This detection system revealed thousand-fold increase in detection sensitivity compare to ELISA. This method could save time and reagent cost compare to common RT-PCR which needs several reactions and several procedures of viral RNA extractions for the same number of samples.

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References

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