Asian-Australasian Journal of Animal Sciences

  • 제19권2호
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  • Pages.153-159
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  • 2006
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Polymorphism of Ghrelin Gene in Twelve Chinese Indigenous Chicken Breeds and Its Relationship with Chicken Growth Traits

  • Li, C.C. (Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine Huazhong Agricultural University) ;
  • Li, K. (Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine Huazhong Agricultural University) ;
  • Li, J. (Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine Huazhong Agricultural University) ;
  • Mo, D.L. (Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine Huazhong Agricultural University) ;
  • Xu, R.F. (Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine Huazhong Agricultural University) ;
  • Chen, G.H. (Laboratory of Animal Genetic-Breeding and Propagation, Colloge of Animal Science and technology, Yangzhou University) ;
  • Qiangba, Y.Z. (Department of Animal Science, Tibet Agriculture and Animal Husbandry College) ;
  • Ji, S.L. (Department of Animal Science, Tibet Agriculture and Animal Husbandry College) ;
  • Tang, X.H. (Department of Animal Science, Tibet Agriculture and Animal Husbandry College) ;
  • Fan, B. (Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine Huazhong Agricultural University) ;
  • Zhu, M.J. (Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine Huazhong Agricultural University) ;
  • Xiong, T.A. (Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine Huazhong Agricultural University) ;
  • Guan, X. (Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine Huazhong Agricultural University) ;
  • Liu, Bang (Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine Huazhong Agricultural University)
  • 투고 : 2005.03.03
  • 심사 : 2005.07.07
  • 발행 : 2006.02.01
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초록

A 2,656 bp fragment of chicken ghrelin gene was cloned and SNPs were detected by PCR-RFLP and Allele Specific PCR (ASP) in 12 Chinese indigenous chicken breeds and a commercial chicken population. The results showed that there were 23 base variations and an amino acid change ($Gln{\rightarrow}Arg$) in cloned chicken ghrelin gene. Three SNPs were confirmed in 13 populations and associations between this gene and growth traits of Tibetan chicken (TC) and Recessive White chicken (RW) were investigated. The results of haplotype analysis revealed that 26 haplotype genotypes were composed of eight haplotypes. The results of $x^2$ tests indicated that there were significant differences between genotypes or haplotype genotype frequencies in some of the breeds or sexes at 0.05 or 0.01 levels. The results of ANOVA revealed that there were significant differences between genotypes or haplotype genotypes on some growth traits of TC and RW chicken breeds at 0.05 or 0.01 levels. Multiple comparisons showed that there were significant associations between genotype CT at site 71 and some growth traits of two chicken breeds and between genotype AG at site 1,215 and body weight at 16 wk of two chicken breeds, and there was a significant association between haplotype genotype CAA/CAG and body weight and shank girth at 16 wk of two chicken breeds.

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참고문헌

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