The Effect of Phytase and Organic Acid on Growth Performance, Carcass Yield and Tibia Ash in Quails Fed Diets with Low Levels of Non-phytate Phosphorus

  • Sacakli, P. (Ankara University, Faculty of Veterinary Medicine, Department of Animal Nutrition) ;
  • Sehu, A. (Ankara University, Faculty of Veterinary Medicine, Department of Animal Nutrition) ;
  • Ergun, A. (Ankara University, Faculty of Veterinary Medicine, Department of Animal Nutrition) ;
  • Genc, B. (Ankara University, Faculty of Veterinary Medicine, Department of Animal Nutrition) ;
  • Selcuk, Z. (Ankara University, Faculty of Veterinary Medicine, Department of Animal Nutrition)
  • Received : 2005.04.11
  • Accepted : 2005.09.01
  • Published : 2006.02.01


An experiment was conducted to investigate the effect of phytase, organic acids and their interaction on body weight gain, feed consumption, feed conversion ratio, carcass yield and tibia ash. A total of 680 three-day old Japanese quail chicks (Coturnix coturnix japonica) were assigned to 20 battery brooders, 34 chicks in each. The experimental period lasted 35 days. The treatment groups employed were: 1) a positive control which included 3.5 g available phosphorus (AP)/kg diet and 10 g Ca/kg diet; 2) a negative control which included 2 g AP/kg diet and 8 g Ca/kg diet, 3) negative control diet supplemented with either 300 FTU phytase/kg diet (phytase) or 4) 2.5 g organic acid (lactic acid+formic acid)/kg diet (organic acid); or 5) 300 FTU phytase/kg diet+2.5 g organic acid/kg diet (phytase+organic acid). All birds were fed with the positive control diet for a week and then transferred to the dietary treatments. At the end of the study, there were no differences (p>0.005) among the groups in body weight, weight gain, feed consumption, feed conversion ratio and carcass yield. Tibia ash, however, was reduced (p<0.001) for quails fed the negative control diet containing a low-level of AP compared to the positive control diet containing adequate AP. The addition of phytase, organic acid or phytase+organic acid to the diets containing the low-level of AP improved (p<0.001) tibia ash. On the other hand, an extra synergistic effect of phytase and organic acid on tibia ash was not determined. This study demonstrated that it may be possible to reduce supplemental level of inorganic P with phytase and/or organic acid supplementation for quail diets without adverse effect on performance and tibia ash.


  1. AOAC. 1984. Official methods of analysis. 14th edn. Association of Official Analytical Chemists, Arlington, Virginia
  2. Boling, S. D., M. W. Douglas, J. L. Snow, C. M. Parsons and D. H. Baker. 2000a. Citric acid does not improve phosphorus utilization in laying hens fed a corn-soybean meal diet. Poult. Sci. 79:1335-1337
  3. Boling, S. D., D. M. Webel, I. Mavromichails, C. M. Parsons and D. H. Baker. 2000b. The effects of citric acid on phytate phosphorus utilization in young chicks and pigs. J. Anim. Sci. 78:682-689
  4. Boling, S. D., J. L. Snow, C. M. Parsons and D. H. Baker. 2001. The effect of citric acid on the calcium and phosphorus requirements of chicks fed corn-soybean meal diets. Poult. Sci. 80:783-788
  5. Cabahug, S., V. Ravindran, P. H. Selle and W. L. Bryden. 1999. Response of broiler chickens to microbial phytase supplementation as influenced by dietary phytic acid and nonphytate phosphorus contents. I. Effects on bird performance and toe ash. Br. Poult. Sci. 40 :660-666
  6. Carpenter, K. J. and K. M. Clegg. 1956. The metabolizable energy of poultry feedingstuffs in relation to their chemical composition. J. Sci. Food Agric. 7:45-51
  7. Cave, N. A. G. 1984. Effect of dietary propionic and lactic acids on feed intake by chicks. Poult. Sci. 63:131-134
  8. Denbow, D. M., V. Ravindran, E. T. Kornegay, Z. Yi and R. M. Hulet. 1995. Improving phosphorus availability in soybean meal for broilers by supplemental phytase. Poult. Sci. 74:1831
  9. Duncan, D. B. 1955. Multiple Range and Multiple F tests. Biometrics, 11:1-42
  10. Gibson, D. M. and A. H. J. Ullah. 1990. Phytase and their actions on phytic acid. In: Inositol Metabolism in Plants. (Ed. D. J. Morre, W. F. Boss, F. A. Loewus) Wiley-Liss, Inc., New York. p. 77
  11. Gordon, R. W. and Sr. D. A. Roland. 1997. Performance of commercial laying hens fed various phosphorus levels, with and without supplemental phytase. Poult. Sci. 76:1172
  12. Jacob, J. P., R. Blair and E. E. Gardlner. 1990. Effect of dietary lactate and glucose on the incidence of sudden death syndrome in male broiler chickens. Poult. Sci. 69:1529-1532
  13. Jongbloed, A. W. and P. A. Kemme. 1990. Apparent digestible phosphorus in the feeding of pigs in relation to availability, requirement and environment. 1. Digestible phosphorus in feedstuffs from plant and animal origin. Netherlands J. Agric. Sci. 38:567
  14. Jongbloed, A. W., Z. Mroz, R. van der Weij-Jongbloed and P. A. Kemme. 2000. The effect of microbial phytase and organic acids and their interactions in diets for growing pigs. Livest. Prod. Sci. 1. 67(1-2):113-122
  15. Kornegay, E. T., D. M. Denbow, Z. Yi and V. Ravindran. 1996. Response of broilers to graded levels of microbial phytase added to maize-soyabean meal-based diets containing three levels of non-phytate phosphorus. Br. J. Nutr. 75:839
  16. Lessard, P., M. R. Lefrançois and J. F. Bernier. 1993. Dietary addition of cellular metabolic intermediates and carcass fat deposition in broilers. Poult. Sci. 72:535-545
  17. Nair, V. C. and Z. Duvnjak. 1991. Phytic acid content reduction in canola meal by various microorganisms in a solid state fermentation process. Acta Biotech. 11:211
  18. Nelson, T. S. 1967. The utilization of phytate P by poultry-A review. Poult. Sci. 46:862
  19. Oberleas, D. 1973. Phytats: Toxicants Occurring Naturally in Foods. National Academy Press. Washington, DC
  20. Pinchasov, Y. and L. S. Jensen. 1989. Effect of short chain fatty acids on voluntary feed of broiler chicks. Poult. Sci. 68:1612-1618
  21. Qian, H., H. P. Veit, E. T. Kornegay, V. Ravindran and D. M. Denbow. 1996. Effect of supplemental phytase and phosphorus on histological and other tibial bone characteristic and performances of broilers fed semi-purified diets. Poult. Sci. 75:618-626
  22. Selle, P. H., V. Ravindran, P. H. Pittolo and W. L. Bryden. 2003. Effects of Phytase Supplementation of Diets with Two Tiers of Nutrient Specifications on Growth Performance and Protein Efficiency Ratios of Broiler Chickens. Asian-Aust. J. Anim. Sci. 16(8):1158-1164
  23. Simons, P. C. M., H. A. J. Versteegh, A. W. Jongbloed, P. A. Kemme, K. D. Bos, M. G. E. Wolters, R. F. Beudeker and V. Verchoor. 1990. Improvement of phosphorus availability by microbial phytase in poultry and pigs. Br. J. Nutr. 64:525-540
  24. Singh, P. K., V. K. Khatta, R. S. Thakur, S. Dey and M. L. Sangwan. 2003. Effects of Phytase Supplementation on the Performance of Broiler Chickens Fed Maize and Wheat Based Diets with Different Levels of Non-Phytate Phosphorus. Asian-Aust. J. Anim. Sci. 16(11):1642-1649
  25. Skinner, J. T. A., A. L. Izat and P. W. Waldroup. 1992a. Effect of removal of supplemental calcium and phosphorus from broiler finisher diets. J. Appl. Poult. Res. 1:42-47
  26. Skinner, J. T. A., A. L. Waldroup and P. W. Waldroup. 1992b. Effect of removal of vitamin and trace mineral supplements from grower and finisher diets on live performance and carcass composition of broilers. J. Appl. Poult. Res. 1 :280-286
  27. Snedecor, G. W. and W. C. Cochran. 1980. Statistical Methods. 7th edn. Iowa State University Press, Ames, Iowa
  28. Sohail, S. S. and Sr. D. A. Roland. 1999. Influence of supplemental phytase on performance of broilers four to six weeks of age. Poult. Sci. 78:550-555
  29. Vohra, P., G. A. Gray and F. H. Kratzer. 1965. Phytic acid-metal complexes. Proceedings of the Society of Experimental Biology and Medicine. 120:447
  30. Waldroup, P. W., R. J. Mitchell and K. R. Hazen. 1974. The phosphorus needs of finishing broilers in relation to dietary nutrient density levels. Poult. Sci. 53:1655-1663
  31. Yalcin, S., I. Onbasilar and B. Kocaoglu. 1997. Blldlrcln besisinde laktik asit kullanlml. Ankara Univ. Vet. Fak. Derg. 44:169-181
  32. Yan, F., J. H. Kersey, J. H. Fritts and P. W. Waldroup. 2003. Phosphorus requirements of broiler chicks six to nine weeks of age as influenced by phytase supplementation. Poult. Sci. 82:294-300

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