Asian-Australasian Journal of Animal Sciences

  • 제19권7호
  • /
  • Pages.931-937
  • /
  • 2006
  • /
  • 1011-2367(pISSN)
  • /
  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Sequence Characterization, Expression Profile, Chromosomal Localization and Polymorphism of the Porcine SMPX Gene

  • Guan, H.P. (Lab of Molecular Biology and Animal Breeding, College of Animal Science and Technology Huazhong Agricultural University) ;
  • Fan, B. (Lab of Molecular Biology and Animal Breeding, College of Animal Science and Technology Huazhong Agricultural University) ;
  • Li, K. (Lab of Molecular Biology and Animal Breeding, College of Animal Science and Technology Huazhong Agricultural University) ;
  • Zhu, M.J. (Lab of Molecular Biology and Animal Breeding, College of Animal Science and Technology Huazhong Agricultural University) ;
  • Yerle, M. (INRA, Laboratoire de Genetique Cellulaire) ;
  • Liu, Bang (Lab of Molecular Biology and Animal Breeding, College of Animal Science and Technology Huazhong Agricultural University)
  • 투고 : 2005.09.05
  • 심사 : 2005.12.29
  • 발행 : 2006.07.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The full-length cDNA of the porcine SMPX gene was obtained by the rapid amplification of cDNA ends (RACE). The nucleotide sequences and the predicted protein sequences share high sequence identity with both human and mouse. The promoter of SMPX was sequenced and then analyzed to find the promoter binding sites. The reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that SMPX has a high level of expression in heart and skeletal muscle, a very low expression in lung and spleen and no expression in liver, kidney, fat and brain. Moreover, SMPX has a differential expression level in skeletal muscle, the expression in 65-day embryos being higher than other stages. The porcine SMPX was mapped to SSCXp24 by using a somatic cell hybrid panel (SCHP) and was found closely linked to SW1903 using the radiation hybrid panel IMpRH. An A/G single nucleotide polymorphism (PCR-RFLP) in the 3'-untranslated region (3'-UTR) was detected in eight breeds. The analysis of allele frequency distribution showed that introduced pig breeds (Duroc and Large White) have a higher frequency of allele A while in the Chinese indigenous pig breeds (Qingping pig, Lantang pig, YushanBlack pig, Large Black-White pig, Small Meishan) have a higher frequencies of allele G. The association analysis using an experimental population (188 pigs), which included two cross-bred groups and three pure-blood groups, suggested that the SNP genotype was associated with intramuscular fat content.

키워드

참고문헌

  1. Bai, Q., C. McGillivray, N. Costa, S. Dornan, G. Evans, M. J. Stear and K. C. Chang. 2003. Development of a porcine skeletal muscle cDNA microarray: analysis of differential transcript expression in phenotypically distinct muscles. BMC Genomics. 4, 8 https://doi.org/10.1186/1471-2164-4-8
  2. Cepica, S., G. Reiner, H. Bartenschlager, G. Moser and H. Geldermann. 2003. Linkage and QTL mapping for Sus scrofa chromosome X. J. Anim. Breed. Genet. 120 (Supp. 1):144-151 https://doi.org/10.1046/j.0931-2668.2003.00436.x
  3. Chevalet, C., J. Gouzy and M. San Cristobal-Gaudy. 1997. Regional assignment of genetic markers using a somatic cell hybrid panel: a WWW interactive program available for the pig genome. Comput. Appl. Biosci. 13:69-73
  4. Ervasti, J. M. 2003. Costameres: the Achilles'heel of Herculean Muscle. J. Biol. Chem. 278:13591-13594 https://doi.org/10.1074/jbc.R200021200
  5. Hicks, G. R. and N. V. Raikhel. 1995. Protein import into the nucleus: An integrated view. Annu. Rev. Cell Dev. Biol. 11:155-188 https://doi.org/10.1146/annurev.cb.11.110195.001103
  6. Janzen, M. A., D. L.Kuhlers, S. B.Jungst and C. F. Louis. 2000. ARPP-16 mRNA is up-regulated in the longissimus muscle of pigs possessing an elevated growth rate. J. Anim. Sci: 78:1475-1484
  7. Jeffery, D. M., Brian. Black, James F. Martin and Eric N. Olson 1996. Mutational Analysis of the DNA Binding, Dimerization, and Transcriptional Activation Domains of MEF2C. Mol. Cell. Biol. 16:2627-2636 https://doi.org/10.1128/MCB.16.6.2627
  8. Kemp, T. J., T. J. Sadusky, M. Simon, R. Brown, M. Eastwood, D. A. Sassoon and G. R. Coulton. 2001. Identification of a novel stretch-responsive skeletal muscle gene (SMPX). Genomics. 72:260-71 https://doi.org/10.1006/geno.2000.6461
  9. Kenealy, S., N. J. Larsen, C. K. Tuggle and M. F. Rothschild. 1998. The very-long-chain acyl-coA dehydrogenase gene maps to pig chromosome 12. J. Anim. Sci. 76:915-916
  10. Kho, C.-J., G. S. Huggins, W. O. Endege, C. Patterson, M. K. Jain, M. E. Lee and E. Haber. 1997. The Polymyositis-Scleroderma Autoantigen Interacts with the Helix-Loop-Helix Proteins E12 and E47. J. Biol. Chem. 272:13426-13431 https://doi.org/10.1074/jbc.272.20.13426
  11. Larzul, C., L. Lefaucheur, P. Ecolan, J. Gogue, A. Talmant, P. Sellier, P. Le Roy and G. Monin. 1997. Phenotypic and genetic parameters for longissimus muscle fiber characteristics in relation to growth, carcass and meat quality traits in Large White pigs. J. Anim. Sci. 75:3126-3137
  12. Lefaucheur, L., F. Edom, P. Ecolan and G. S. Butler-Browne. 1995. Pattern of muscle fiber type formation in the pig. Dev. Dyn. 203:27-41 https://doi.org/10.1002/aja.1002030104
  13. McCoard, S. A., S. C. Fahrenkrug, L. J. Alexander, B. A. Freking, G. A. Rohrer, T. H. Wise and J. J. Ford. 2002. An integrated comparative map of the porcine X chromosome. Anim. Genet. 33:178-185 https://doi.org/10.1046/j.1365-2052.2002.00878.x
  14. Milan, D., R. Hawken, C. Cabau, S. Leroux, C. Genet, Y. Lahbib, G. Tosser, A. Robic, F. Hatey, L. Alexander, C. Beattie, L. Schook, M. Yerle and J. Gellin. 2000. IMpRH server: an RH mapping server available on the web. Bioinformatics. 16:558-559 https://doi.org/10.1093/bioinformatics/16.6.558
  15. Palmer, S., N. Groves, A. Schindeler, T. Yeoh, C. Biben, C. C. Wang, D. B. Sparrow, L. Barnett, N. A. Jenkins, N. G. Copeland, F. Koentgen, T. Mohun and R. P. Harvey. 2001. The Small Muscle-specific Protein Csl Modifies Cell Shape and Promotes Myocyte Fusion in Insulin-like Growth Factor 1-dependent Manner. J. Cell Biol. 153:985-998 https://doi.org/10.1083/jcb.153.5.985
  16. Pan, P. W., S. H. Zhao, M. Yu, B. Liu, T. A. Xiong and K. Li. 2003. Identification of differentially expressed genes in the longissimus dorsi muscle tissue between duroc and erhualian pigs by mRNA differential display. Asian-Aust. J. Anim. Sci. 16:1066-1070 https://doi.org/10.5713/ajas.2003.1066
  17. Patzak, D., O. Zhuchenko, C.-C. Lee and M. Wehnert. 1999. Identification, mapping and genomic structure of a novel Xchromosomal human gene (SMPX) encoding a small muscular protein. Hum. Genet. 105:506-512 https://doi.org/10.1007/s004390051138
  18. Pollock, R. and R. Treisman. 1991. Human SRF-related proteins: DNA-binding properties and potential regulatory targets. Genes Dev. 5:2327-2341 https://doi.org/10.1101/gad.5.12a.2327
  19. Saadane, N., L. Alpert and L. E. Chalifour. 1999. Expression of immediate early genes, GATA-4, and Nkx-2.5 in adrenergicinduced cardiac hypertrophy and during regression in adult mice. Br. J. Pharmacol. 127:1165-1176 https://doi.org/10.1038/sj.bjp.0702676
  20. Schindeler, A., L. Lavulo and R. P. Harvey. 2005. Muscle costameric protein, Chisel/Smpx, associates with focal adhesion complexes and modulates cell spreading in vitro via a Rac1/p38 pathway. Exp. Cell Res. 307:367-380 https://doi.org/10.1016/j.yexcr.2005.04.006
  21. Shaw, G. and R. Kamen. 1986. A conserved AU sequence from the 3'-untranslated region of GM-CSF mRNA mediates selective mRNA degradation. Cell. 46:659-667 https://doi.org/10.1016/0092-8674(86)90341-7
  22. Yang, J., M. Yu, B. Liu, B. Fan, M. Zhu, T. Xiong and K. Li. 2005. Clone and initial analysis of porcine MPDU1 gene. Asian-Aust. J. Anim. Sci. 18:1237-1241 https://doi.org/10.5713/ajas.2005.1237
  23. Yerle, M., G. Echard, A. Robic, A. Mairal, C. Dubut-Fontana, J. Riquet, P. Pinton, D. Milan, Y. Lahbib-Mansais and J. Gellin. 1996. A somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics. Cytogenet. Cell Genet. 73:194-202 https://doi.org/10.1159/000134338
  24. Yerle, M., P. Pinton, A. Robic, A. Alfonso, Y. Palvadeau, C. Delcros, R. Hawken, L. Alexander, L. B. Beattie, D. Milan and J. Gellin. 1998. Construction of a wholegenome radiation hybrid panel for high-resolution gene mapping in pigs. Cytogenet. Cell Genet. 82:182-188 https://doi.org/10.1159/000015095
  25. Valdez, M. R., J. A. Richardson, W. H. Klein and E. N. Olson. 2000. Failure of Myf5 to support myogenic differentiation without myogenin, MyoD and MRF4. Dev. Biol. 219:287-298 https://doi.org/10.1006/dbio.2000.9621
  26. Wingender, E., X. Chen, R. Hehl, H. Karas, I. Liebich, V. Matys, T. Meinhardt, M. Prub, I. Reuter and F. Schacherer. 2000. TRANSFAC: an integrated system for gene expression regulation. Nucleic Acids Res. 28:316-319 https://doi.org/10.1093/nar/28.1.316

피인용 문헌

  1. Characterization and Mapping of the Bovine FBP1 Gene vol.20, pp.9, 2007, https://doi.org/10.5713/ajas.2007.1319
  2. The Porcine FoxO1, FoxO3a and FoxO4 Genes: Cloning, Mapping, Expression and Association Analysis with Meat Production Traits vol.20, pp.5, 2006, https://doi.org/10.5713/ajas.2007.627