Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Molecular Characterization and Expression Patterns of Porcine Eukaryotic Elongation Factor 1 A

  • Wang, H.L. (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agricultural Sciences) ;
  • Wang, H. (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agricultural Sciences) ;
  • Zhu, Z.M. (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agricultural Sciences) ;
  • Yang, S.L. (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agricultural Sciences) ;
  • Fen, S.T. (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agricultural Sciences) ;
  • Li, Kui (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agricultural Sciences)
  • Received : 2005.11.18
  • Accepted : 2006.02.21
  • Published : 2006.07.01
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Abstract

The eukaryotic elongation factor 1 A (EEF1A) participates in protein synthesis by forming the eEF1A GTP tRNA complex to deliver aminoacyl-tRNA to the A site of ribosomes. This study described cDNA sequences and partial genomic structure of porcine EEF1A1. The porcine EEF1A1 gene encoded a protein with 462 amino acids, which shared complete homology with human, chimpanzee and dog. The temporal expression pattern showed the diversity of EEF1A1 level in mRNA was relatively minor in prenatal embryo skeletal muscle, however, the expression decreased during aging after birth in skeletal muscle of the Chinese Tongcheng pig. The spatial expression patterns indicated that the gene expressed in skeletal muscle, heart, lung, liver, kidney, fat and spleen. In addition, we assigned the gene to porcine chromosome 1 using a radiation hybrid panel.

Keywords

References

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