Asian-Australasian Journal of Animal Sciences

  • 제19권8호
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  • Pages.1065-1070
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  • 2006
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Identification of Novel SNPs with Effect on Economic Traits in Uncoupling Protein Gene of Korean Native Chicken

  • Oh, J.D. (Genomic Informatics Center, Hankyong National University) ;
  • Kong, H.S. (Animal Products Grading Service) ;
  • Lee, J.H. (Genomic Informatics Center, Hankyong National University) ;
  • Choi, I.S. (Genomic Informatics Center, Hankyong National University) ;
  • Lee, S.J. (Genomic Informatics Center, Hankyong National University) ;
  • Lee, S.G. (Genomic Informatics Center, Hankyong National University) ;
  • Sang, B.D. (National Livestock Research Institute, RDA) ;
  • Choi, C.H. (National Livestock Research Institute, RDA) ;
  • Cho, B.W. (Deptartment of Animal Science, Miryang National University) ;
  • Jeon, G.J. (Genomic Informatics Center, Hankyong National University) ;
  • Lee, H.K. (Dept. of Biotechnology, Hankyong National University)
  • 투고 : 2005.11.18
  • 심사 : 2006.03.02
  • 발행 : 2006.08.01
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초록

The avian uncoupling protein (avUCP) is a member of the mitochondrial transporter superfamily that uncouples proton entry in the mitochondrial matrix from ATP synthesis. The sequencing analysis method was used to identify nucleotide polymorphisms within the avUCP gene in Korean native chicken (KNC). This study identified ten single nucleotide polymorphisms (SNPs) in the avUCP gene. We analyzed the SNPs of the avUCP gene to investigate whether polymorphism in the gene might be responsible for quantitative variations in economic traits in KNC. Three significant polymorphic sites for economic traits were avUCP C+282T (mean body weight, p<0.05), avUCP C+433T (daily percent lay, p<0.05), and avUCP T+1316C (daily percent lay, p<0.05). The frequency of each SNP was 0.125 (C+282T in avUCP gene exon 1 region), 0.150 (C+433T in avUCP gene intron 1 region), and 0.15 (T+1316C in avUCP gene exon 3 region), respectively. Among the identified SNPs, one pair of SNPs (genotype CC, C+282T and TT, avUCP C+433T) showed the highest daily percent lay (p<0.05) and mean body weight (p<0.05) and the frequency was 0.067. This study of the avUCP gene could be useful for genetic studies of this gene and selection on economic traits for KNC.

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참고문헌

  1. Boss, O., S. Samec, A. Paoloni-Giacobino, C. Rossier, A. Dulloo, J. Seydoux, P. Muzzin and J. P. Giacobino. 1997. Uncoupling protein-3: a new member of the mitochondrial carrier family with tissue-specific expression. FEBS Lett. 408:39-42 https://doi.org/10.1016/S0014-5793(97)00384-0
  2. Bouillaud, F., E. Couplan, C. Pecqueur and D. Ricquier. 2001. Homologues of the uncoupling protein from brown adipose tissue (UCP-1):UCP2, UCP3, BMCP1 and UCP4. Biochim. Biophys. Acta. 1:107-119
  3. Cassard, A. M., F. Bouillaud, M. G. Mattei, E. Hentz, S. Raimbault, M. Thomas and D. Ricquier. 1990. Human uncoupling protein gene: structure, comparison with rat gene, and assignment to the long arm of chromosome 4. J. Cell Biochem. 43:255-264 https://doi.org/10.1002/jcb.240430306
  4. Clement, K., J. Ruiz, A. M. Cassard-Doulcier, F. Bouillaud, D. Ricquier, A. Basdevant, B. Guy-Grand and P. Froguel. 1996. Additive effect of A$\rightarrow$G (-3,826) variant of the uncoupling protein gene and the Trp64Arg mutation of the beta 3-adrenergic receptor gene on weight gain in morbid obesity. Int. J. Obes. Relat. Metab. Disord. 20:1062-1066
  5. Collin, A., J. Buyse, P. van As, V. M. Darras, R. D. Malheiros, V. M. Moraes, G. E. Reyns, M. Taouis and E. A. Decuypere. 2003. Cold-induced enhancement of avian uncoupling protein expression, heat production, and triiodothyronine concentrations in broiler chicks. General and Comparative Endocrinol. 130:70-77 https://doi.org/10.1016/S0016-6480(02)00571-3
  6. Duchamp, C., F. Marmonier, F. Denjean, J. Lachuer, T. P. D. Eldershaw, J. L. Rouanet, A. Morales, R. Meister, C. Benistant, D. Roussel and H. Barre. 1999. Regulatory, cellular and molecular aspects of avian muscle non-shivering thermogenesis. Ornis. Fenn. 76:151-165
  7. Esterbauer, H., C. Schneitler, H. Oberkofler, C. Ebenbichler, B. Paulweber, F. Sandhofer, G. Ladurner, E. Hell, A. D. Strosberg, J. R. Patsch, F. Krempler and W. Patsch. 2001. A common polymorphism in the promoter of UCP2 is associated with decreased risk of obesity in middle-aged humans. Nat. Genet. 28:178-183 https://doi.org/10.1038/88911
  8. Fleury, C., M. Neverova, S. Collins, S. Raimbault, O. Champigny, C. Levy-Meyrueis, F. Bouillaud, M. F. Seldin, R. S. Surwit, D. Riquier and C. H. Warden. 1997. Uncouping protein-2: a novel gene linked to obesity and hyperinsulinemia. Nat. Genet. 15:269-272 https://doi.org/10.1038/ng0397-269
  9. Fogelholm, M., R. Valve, K. Kukkonen-Harjula, A. Nenonen, V. Hakkarainen, M. Laakso and M. Uusitupa. 1998. Additive effects of the mutations in the beta3-adrenergic receptor and uncoupling protein-1 genes on weight loss and weight maintenance in Finnish women. J. Clin. Endocrinol. Metab. 83:4246-4250 https://doi.org/10.1210/jc.83.12.4246
  10. Fumeron, F., I. Durack-Bown, D. Betoulle, A. M. Cassard-Doulcier, S. Tuzet, F. Bouillaud, J. C. Melchior, D. Ricquier and M. Apfelbaum. 1996. Polymorphisms of uncoupling protein (UCP) and beta 3 adrenoreceptor genes in obese people submitted to a low calorie diet. Int. J. Obes. Relat. Metab. Disord. 20:1051-1054
  11. Gabarrou, J. F., P. A. Geraert, M. Picard and A. Bordas. 1997. Diet-induced thermogenesis in cockerelsid modulated by genetic selection for high or low residual feed intake. J. Nutr. 127:2371-2376
  12. Garlid, K. D., D. E. Orosz, M. Modriansky, S. Vassanelli and P. J. Jezek. 1996. On the mechanism of fatty acid-induced proton transport by mitochondrial uncoupling protein. J. Biol. Chem. 271:2615-2620 https://doi.org/10.1074/jbc.271.5.2615
  13. Gimeno, R. E., M. Dembski, X. Weng, N. Deng, A. W. Shyjan, C. J. Gimeno, F. Iris, S. J. Ellis, E. A. Woolf and L. A. Tartaglia. 1997. Cloning and characterization of an uncoupling protein homolog. A potential molecular mediator of human thermogenesis. Diabetes. 46:900-906 https://doi.org/10.2337/diabetes.46.5.900
  14. Hayakawa, T., Y. Nagai, M. Taniguchi, H. Yamashita, T. Takamura, T. Abe, G. Nomura and K. Kobayashi. 1999. Phenotypic characterization of the beta3-adrenergic receptor mutation and the uncoupling protein 1 polymorphism in Japanese men. Metabolism. 48:36-40
  15. Heilbronn, L. K., K. L. Kind, E. Pancewicz, A. M. Morris, M. Noakes and P. M. Clifton. 2000. Association of -3826G variant in uncoupling protein-1 with increased BMI in overweight Australian women. Diabetol. 43:242-244 https://doi.org/10.1007/s001250050036
  16. Herrmann, S. M., J. G. Wang, E. Staessen, J. A. Kertmen, K. Schmidt-Petersen, W. Zidek, M. Paul and E. Brand. 2003. Uncoupling protein 1 and 3 polymorphisms are associated with waist-to-hip ratio. J. Mol. Med. 81:327-332 https://doi.org/10.1007/s00109-003-0431-1
  17. Kogure, A., T. Yoshida, N. Sakane, T. Umekawa, Y. Takakura and M. Kondo. 1998. Synergic effect of polymorphisms in uncoupling protein 1 and beta3-adrenergic receptor genes on weight loss in obese Japanese. Diabetol. 41:1399 https://doi.org/10.1007/s001250051084
  18. Krempler, F., H. Esterbauer, R. Weitgasser, C. Ebenbichler, J. R. Patsch, K. Miller, M. Xie, V. Linnemayr, H. Oberkofler and W. Patsch. 2002. A functional polymorphism in the promoter of UCP2 enhances obesity risk but reduces type 2 diabetes risk in obese middle-aged humans. Diabetes 51:3331-3335 https://doi.org/10.2337/diabetes.51.11.3331
  19. Miller, S. A., D. D. Dykes and H. F. Polesky. 1988. A simple salting out procedure for extracting DNA from human nucleated cell. Nucleic Acids Res. 16:1215 https://doi.org/10.1093/nar/16.3.1215
  20. Monica, D., P. Lucia, C. Marina, A. M. Maria, F. Simona, A. Francesco, S. Angela, L. Davide, S. Paolo, F. Massimo, P. Michele, E. P. Antonio, L. Renato, P. Francesco, F. Franco and S. Giorgio. 2004. The -866A/A genotype in the promoter of the human uncoupling protein 2 gene is associated with insulin resistance and increased risk of type 2 diabetes. Am. Diabetes Assoc. 53:1905-1910
  21. Oeffner, F., D. Bornholdt, A. Ziegler, A. Hinney, T. Gorg, G. Gerber, H. P. Goldschmidt, W. Siegfried, A. Wright, J. Hebebrand and K. H. Grzeschik. 2000. Significant association between a silent polymorphism in the neuromedin B gene and weight in German children and adolescents. Acta Diabetol. 37:93-101 https://doi.org/10.1007/s005920070026
  22. Oppert, J. M., M. C. Vohl, M. Changon, F. T. Dionne, A. M. Cassard-Doulcier, D. Ricquier, L. Perusse and C. Bouchard. 1994. DNA polymorphism in the uncoupling protein (UCP) gene and human body fat. Int. J. Obes. Relat. Metab. Disord. 18:526-531
  23. Pihlajamaki, J., J. Rissanen, R. Valve, S. Heikkinen, L. Karjalainen and M. Laakso. 1998. Different regulation of free fatty acid levels and glucose oxidation by the Trp64Arg polymorphism of the beta3-adrenergic receptor gene and the promoter variant (A-3826G) of the uncoupling protein 1 gene in familial combined hyperlipidemia. Metabol. 47:1397-1402 https://doi.org/10.1016/S0026-0495(98)90312-2
  24. Proenza, A. M., C. M. Poissonnet, M. Ozata, S. Ozen, S. Guran, A. Palou and A. D. Strosberg. 2000. Association of sets of alleles of genes encoding beta3-adrenoreceptor, uncoupling protein 1 and lipoprotein lipase with increased risk of metabolic complications in obesity. Int. J. Obes. Relat. Metab. Disord. 24:93-100 https://doi.org/10.1038/sj.ijo.0801091
  25. Raimbault, S., S. Dridi, F. Denjean, J. Lachuer, E. Couplan, F. Bouillaud, A. Bordas, C. Duchamp, M. Taouis and D. Ricquier. 2001. An coupling protein homologue putatively involved in facultative thermogenesis in birds. Biochem. J. 345:161-179
  26. Shihara, N., K. Yasuda, T. Moritani, H. Ue, M. Uno, T. Adachi, K. Nunoi, Y. Seino, Y. Yamada and K. Tsuda. 2001. Synergistic effects of polymorphisms of uncoupling protein 1 and beta3-adrenergic receptor genes on autonomic nervous system activity. Int. J. Obes. Relat. Metab. Disord. 25:761-766
  27. Sivenius, K., R. Valve, V. Lindi, L. Niskanen, M. Laakso and M. Uusitupa. 2000. Synergistic effect of polymorphisms of uncoupling protein 1 and beta3-adrenergic receptor genes on long-term body weight change in Finnish type 2 diabetic and non-diabetic control subjects. Int. J. Obes. Relat. Disord. 24:514-519 https://doi.org/10.1038/sj.ijo.0801194
  28. Sophie, L. F., L. S. Catherine, D. S. Christine and B. Pierre. 2004. The common -866G/A polymorphism in the promoter of uncoupling protein 2 is associated with increased carbohydrate and decreased lipid oxidation in juvenile obesity. Am. Diabet. Assoc. 53:235-239
  29. Vidal-Puig, A., G. Solanes, D. Grujic, J. S. Flier and B. B. Lowell. 1997. UCP3: an coupling protein homologue expressed preferentially and abundantly in skeletal muscle and brown adipose tissue. Biochem. Biophys. Res. Commun. 235:79-82 https://doi.org/10.1006/bbrc.1997.6740

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