Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Increased osteopontin expression in activated glial cells in experimental autoimmune encephalomyelitis

자가면역성 뇌척수염 흰쥐의 활성화된 신경아교세포에서 증가된 osteopontin의 발현

  • Park, Suk-jae (Department of Veterinary Medicine and Applied Radiological Science Institute, College of Applied Life Science, Cheju National University) ;
  • Hwang, In-sun (Department of Veterinary Medicine and Applied Radiological Science Institute, College of Applied Life Science, Cheju National University) ;
  • Kim, Gyu-beom (Department of Veterinary Medicine and Applied Radiological Science Institute, College of Applied Life Science, Cheju National University) ;
  • Shin, Tae-kyun (Department of Veterinary Medicine and Applied Radiological Science Institute, College of Applied Life Science, Cheju National University) ;
  • Jee, Young-heun (Department of Veterinary Medicine and Applied Radiological Science Institute, College of Applied Life Science, Cheju National University)
  • 박석재 (제주대학교 농업생명과학대학 수의학과) ;
  • 황인선 (제주대학교 농업생명과학대학 수의학과) ;
  • 김규범 (제주대학교 농업생명과학대학 수의학과) ;
  • 신태균 (제주대학교 농업생명과학대학 수의학과) ;
  • 지영흔 (제주대학교 농업생명과학대학 수의학과)
  • Accepted : 2006.07.14
  • Published : 2006.09.30
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Abstract

Experimental autoimmune encephalomyelitis (EAE) is a disease model of multiple sclerosis (MS) that is characterized by remittance and relapse of the disease and autoimmune and demyelinating lesions in the central nervous system (CNS). Autoimmune inflammation is maintained by secretion of a large number of protein. Previous studies have suggested that transcripts encoding osteopontin (OPN) are frequently detected in the mRNA population of MS plaques. To elucidate the functional role of OPN in initiation and development of EAE, we examined the expression and localization of OPN in the spinal cord during acute EAE. We demonstrated that OPN significantly increased at the early stage of EAE and slightly declined thereafter by western blot analysis. An immunohistochemical study revealed that OPN was constitutively expressed in some glial cells (microglia, astrocytes) of white matter and neurons in the CNS of control rats. OPN expression was shown to be increased in the same cells at the early and peak stage of EAE. To identity cells expressing OPN by double-immunofluorescence labeling, we labeled rat spinal cord sections for OPN with a monoclonal OPN antibody and with mAbs for astrocyte (GFAP), microglia/macrophage (OX42)-specific markers. The major cell types of OPN-expressing cells were activated astrocytes and microglia in the adjacent inflammatory lesions. Interestingly, OPN was mainly expressed in the end feet of astrocytes around vascular cell adhesion molecule-1 (VCAM-1) expressing endothelial cells of CNS blood vessel. These findings suggest that increased levels of OPN in activated glial cell may play an important role in the recruitment of inflammatory cells into the CNS parenchyma during EAE.

Keywords

References

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