Sequence analysis of VP2 gene of infectious bursal disease virus field isolate and vaccine strains

Infectious bursal disease virus 국내분리주 및 백신주의 VP2 gene의 비교분석

  • Jin, Ji-Dong (School of Veterinary Medicine, Kangwon National University) ;
  • Kang, Zheng-Wu (School of Veterinary Medicine, Kangwon National University) ;
  • Kim, Sun-Joong (College of Veterinary Medicine, Seoul National University) ;
  • Kwon, Hyuk-Moo (School of Veterinary Medicine, Kangwon National University) ;
  • Hahn, Tae-Wook (School of Veterinary Medicine, Kangwon National University)
  • Accepted : 2006.09.11
  • Published : 2006.09.30

Abstract

The VP2 full gene of Korean infectious bursal disease virus(IBDV) strain, SH/92, three attenuated vaccine strains, Bur706, Bursine-2 and CEV/AC strains, were amplified by reverse transcriptase-polymerase chain reaction and sequenced and compared with published VP2 gene sequences of IBDVs. The VP2 nucleotide sequence similarity between SH/92 and three vaccine stains was 95.6~96.5% whereas the nucleic acid similarity among three vaccine strains was 97.5~98.5%. The amino acid sequence similarity of VP2 of SH/92 compared with three vaccine strains was between 94.4 and 97.6% while the amino acid similarity among three vaccine strains was between 97.4 and 98.4%. The amino acid similarity between SH/92 and classical virulent strain, 52/70 and STC strain was 96.4 and 96.5%, respectively. The serine-rich heptapeptide was conserved in CEVAC and Bursine-2 as well as SH/92 but not in Bur706. The phylogenetic tree developed from amino acid sequences showed that SH/92 was categorized with vv IBDVs(HK46, OKYM, KKI, UPM94/273, SH95) in one branch while three vaccine strains were catagorized with STC strain in the other branch.

Keywords

Acknowledgement

Supported by : 농림부

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