Quinone profile를 이용한 하천생태계의 미생물군집구조 해석

Analysis of Microbial Community Structure in River Ecosystem Using Quinone Profiles

  • 투고 : 2006.06.15
  • 심사 : 2006.09.14
  • 발행 : 2006.10.15

초록

The differences in microbial community structures between planktonic microorganism and biofilm in rivers were investigated using respiratory quinone profiles. The compositions of microbial quinone for 4 tributaries of the Kyongan Stream located in/flowing through Yongin City, Gyeonggi-Do were analyzed. Ubiquinone(UQ)-8, UQ-9, menaquinone(MK)-6 and Plastoquinone(PQ)-9 were observed in all samples of planktonic microorganism and biofilm for the sites investigated, Most planktonic microorganism and biofilm had UQ-8(15 to 30%) and PQ-9(over 30%) as the dominant quinone type. These results indicated that oxygenic phototrophic microbes(cyanobacteria and/or eukaryotic phytoplankton) and UQ-8 containing proteobacteria constituted major microbial populations in the river. The quinone concentration in the river waters tested, which reflects the concentration of planktonic microorganisms, increases with increasing DOC. Further research into this is required. The microbial diversities of planktonic microorganism and biofilm calculated based on the composition of all quinones were in the range from 4.2 to 7.5, which was lower than those for activated sludge(ranging from 11 to 14.8) and soils(ranging from 13.4 to 16.8). The use of quinone profile appears to be a useful tool for the analysis of microbial community structure in river.

키워드

참고문헌

  1. 나진성, 김기태, 김상돈 (2005) 독성 반응곡선을 이용한 수계 주요 오염물질의 혼합독성평가, 대한환경공학회지, 27(1). pp.67-74
  2. 배연재, 원두희, 이웅재, 승현우 (2003) 하천 생태계에 대한 환경영향평가 기법과 생물다양성 관리시스템의 개발 및 적용, Korean J. Environ. Biol., 21(3), pp. 223-233
  3. 신재기, 박경미, 황순진, 조경제 (2001) 경안천과 팔당호에서 총세균수의 분포 및 동태, Korean J. Limnol., 34(2), pp.119-125
  4. 이영옥, 박지은, 신승필, 안영희 (2003) FISH법을 이용한 낙동강 상.중.하류의 세균군집 구조 분석, 한국물환경학회지,19(3), pp,311-320
  5. 임병란, 홍준석, 안규홍 (2003) 퀴논프로화일을 이용한 하수처리장 공정내 미생물군집구조 해석, 대한환경공학회 추계학술연구발표회 논문집, paper on CD-ROM
  6. 조경제, 신제기 (1998) 낙동강 하류에서 동.하계 무기 N. P 영양염류와 식물플랑크톤의 동태, Korean J. Limnol., 31(1), pp.67-75
  7. 한강유역환경청 (2001) 경안천 유역의 오염부하량조사 보고서
  8. Fujie, K, Hu, H-Y., Tanaka, H., Urano, K, Saito, K, and Katayama, A. (1998) Analysis of Respiratory Quinones in Soil for Characterization of Microbiota, Soil Sci. Plant Nutr., 44(3), pp. 393-404 https://doi.org/10.1080/00380768.1998.10414461
  9. Helen, C., Sarakinos, N.B., Paul, A.W., Joseph, B.R. (2000) Correspondence between Whole Effluent Toxicity and the Presence of Priority Substance in Complex Industrial Effluents, Environ. Toxicol. Chem., 19(1), pp. 63-71 https://doi.org/10.1002/etc.5620190107
  10. Hiraishi, A. (1999) Isoprenoid Quinones as Biomarkers of Microbial Populations in the Environment, J. Biosci. Bioeng., 88, pp. 449-460 https://doi.org/10.1016/S1389-1723(00)87658-6
  11. Horowitz, A.J. (1991) A primer on sediment-trace element chemistry, Lewis Publishers, Chelsea
  12. Hu, H.-Y., Fujie, K and Urano, K. (1999a) Development of a Novel Solid Phase Extraction .vlcthod for the Analysis of Bacterial Quinones in Activated Sludge with a Higher Reliability, J. Biosci. Bioeng. 87(3), pp. 378-382 https://doi.org/10.1016/S1389-1723(99)80049-8
  13. Hu, H-Y., Fujie, K., Nakagome, H., Urano, K., and Katayama, A. (1999b) Quantitative Analyses of the Change in Microbial Diversity in a Bioreactor for Wastewater Treatment Based on Respiratory Quinones, Wat. Res., 33(15), pp. 3263-3270 https://doi.org/10.1016/S0043-1354(99)00044-5
  14. Hu, H.-Y., Lim, B.-R., Goto, N., Bhupathiraju, V. K, and Fujie, K. (2001) Characterization of Microbial Community in an Activated Sludge Process Treating Domestic Wastewater using Quinone Profiles, Wat. Sci. Tech., 43, pp.99-106
  15. Kunihiro, T., Hu, H.-Y., Lim, B.-R., Goto, N. and Fujie, K. (2002) Analysis of the Differences in Microbial Community Structures between Suspended and Sessile Microorganisms in River based on Quinone Profile, J. Gen. Appl. Microbiol., 48(1), pp. 35-41 https://doi.org/10.2323/jgam.48.35
  16. Lim, B-R., Hu, H.-Y., Huang, X. and Fujie, K .(2002) Effect of Seawater on the Treatment Performance and Microbial Population in a Biofilter Treating Coke-oven Wastewater, Process Biocbemistry. 37, pp. 943-948
  17. Lim, B-R. and Ahn, K.-H. (2004) Analysis of Microbial Community Structure in a Biofilm on Membrane Surface in the Submerged Membrane Bioreactor Treating Domestic Wastewater on the Basis of Respiratory Quinone Profiles, J. Gen. Appl. Microbiol., 50(4), pp. 197-202 https://doi.org/10.2323/jgam.50.197
  18. Ying, a., Omura, T., Umita, T., Aizawa, J., Sato, Y., and Kaito, T., (1993) Aquatic Environmental Evaluation by the Benthic Animal Species in the River with Non-point Pollution Source, 日本水環境學曾, 16(11), pp.804-814