한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제34권2호
  • /
  • Pages.150-157
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  • 2006
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Bacillus sp. SMMJ-2의 Keratinolytic protease 생산최적조건

Optimization of Keratinolytic Protease Productions from Bacillus sp. SMMJ-2

  • 발행 : 2006.06.01
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초록

스웨덴 스톡홀름의 항구부근 토양시료로부터 분리한 Bacillus sp. SMMJ-2는 단백질 분해활성이 높은 균주로써 특히 keratinolytic protease 생산성이 우수한 균주이다. 효소생산을 위한 최적 배지조성은 0.7% $K_2HPO_4$, 0.2% $KH_2PO_4$, 1.0% fructose, 1.2% soybean meal(roasted), 그리고 0.01% $Na_2CO_3$ 이었으며 배양초기 pH는 7.0, 배양온도는 30$^{\circ}C$에서 200 rpm조건일 때 효소생산이 가장 우수하였으며 이때의 활성은 105 units/ml/min로 조사되었다.

Bacillus sp. SMMJ-2 producing extracellular keratinolytic protease was isolated from the Swedish soils. The optimal culture conditions for production of keratinolytic protease by Bacillus sp. SMMJ-2 were investigated. The optimal medium compositions for the keratinolytic protease production were 0.7% $K_2HPO_4$, 0.2% $KH_2PO_4$, 1.0% fructose,1.2% soybean meal (roasted), and 0.01% $Na_2CO_3$. Optimal initial pH and temperature for the production of keratinolytic protease were 7.0 and 30$^{\circ}C$, respectively. The keratinolytic protease production showed a maximum of 105 units/ml/min after 72 hours cultivation under the optimal culture conditions.

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