Identification of the Antioxidative Function of Metallothionein by Oxidation of NADPH and Production of Nitrite

NADPH의 산화반응과 아질산 생성반응에 의한 Metallothionein 의 항산화적 기능 확인

  • Kim Kwan-Chun (Dept. of Environmental Administration, Gwangju Health College) ;
  • Kim Joon-Tae (Dept. of Environmental Administration, Gwangju Health College) ;
  • Kim Hee-Joung (Dept. of Chemistry, Chosun University)
  • 김관천 (광주보건대학 환경행정과) ;
  • 김준태 (광주보건대학 환경행정과) ;
  • 김희정 (조선대학교 화학과)
  • Published : 2006.03.01

Abstract

Metallothioneins(MTs) belong to the class of low molecular weight proteins. Recently, it has been suggested that MTs may playa direct role in cellular defense against oxidative stress by functioning as antioxidants. Oxidative damage to different cellular components makes a major contribution to many pathogenenesses. Several studies have demonstrated that MT is able to quench a wide range of reactive oxygen species at a higher efficiency than other well known antioxidants such as superoxide dismutate(SOD). The present study was designed to evaluate the effect of MT on the activities of the reactive oxygen species removal system. MT showed the scavenging of superoxide in the SOD assay system in the presence or absence of SOD. When MT was added to nicotinamide adenine dinucleotide phosphate(NADPH) oxidation system in presence of fixed amount of SOD increase the breakdown rate of superoxide. When MT was added to the system that form nitrite from hydroxylammonium chloride, the formation of nitrite was inhibit. We concluded that the function of MT as antioxidant might have an effect on the level of superoxide scavenging.

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References

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