Toxicological Research

  • 제22권2호
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  • Pages.75-85
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  • 2006
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  • 1976-8257(pISSN)
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  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
권호 표지

T-2 toxin을 투여한 닭에서 Comet assay 방법을 이용한 DNA 손상 평가와 독성

Assessment of DNA Damage using an Alkaline Single Cell Gel Electrophoresis (SCGE) Comet Assay and Toxic Effects in Chickens by T-2 Toxin Treatment

  • 하대식 (경남보건 환경연구원) ;
  • 허정호 (경남 축산 진흥연구소 남부지소) ;
  • 이국천 (경남 축산 진흥연구소 남부지소) ;
  • 조명희 (경남 축산 진흥연구소 남부지소) ;
  • 김국헌 (경남 축산 진흥연구소 남부지소) ;
  • 김충희 (진주산업대학교 동물생명과학과) ;
  • 류재두 (국립동물 검역원 부산지원) ;
  • 이승환 (경상대학교 수의과 대학 (동물의학 연구소)) ;
  • 김곤섭 (경상대학교 수의과 대학 (동물의학 연구소)) ;
  • 김의경 (경상대학교 수의과 대학 (동물의학 연구소)) ;
  • 김종수 (경상대학교 수의과 대학 (동물의학 연구소))
  • Hah Dae-Sik (Gyeongnam provincial Government Institute of Health Environment) ;
  • Heo Jung-Ho (Gyeongnam Livestock Promotion Institute South-branch) ;
  • Lee Kuk-Cheon (Gyeongnam Livestock Promotion Institute South-branch) ;
  • Cho Myung-Heui (Gyeongnam Livestock Promotion Institute South-branch) ;
  • Kim Kuk-Hun (Gyeongnam Livestock Promotion Institute South-branch) ;
  • Kim Chung-Hui (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Lue Jae-Du (National Veterinary Research and Quarantine service Busan Regional Office) ;
  • Lee Seung-Hwan (College of Veterinary Medicine and Institute of Animal Science, Gyeongsang National University) ;
  • Kim Gon-Sup (College of Veterinary Medicine and Institute of Animal Science, Gyeongsang National University) ;
  • Kim Eui-Gyung (College of Veterinary Medicine and Institute of Animal Science, Gyeongsang National University) ;
  • Kim Jong-Shu (College of Veterinary Medicine and Institute of Animal Science, Gyeongsang National University)
  • 발행 : 2006.06.01
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초록

This study was designed to evaluate the possible DNA damaging effects of T-2 toxin using an alkaline single cell gel electrophoresis (SCGE) comet assay and also to investigate toxic effects in chickens. A total of 20 chickens were used in these experiments. Graded concentrations of dietary T-2 toxin (0, 4, 8, and $16{\mu}g/g$ of diet) were given to groups of 5 broiler chickens. In comet assay, The DNA damage was analysed by the tail extent moment (TEM) and tail length (TL), which were used as markers of DNA strand breaks in SCGE. A significant dose-dependent increase in the extent of DNA migration as well as in the percentage of cells with tails was observed after treatment with T-2 toxin (P<0.05). Treatment with the low T-2 toxin ($4{\mu}/g$ of diet) induced a relatively low level of DNA damage in comparison with the high T-2 toxin ($16{\mu}/g$ of diet) group. The growth rate was significantly reduced by concentrations of 8, and $16{\mu}/g$ of diet (P < 0.05). The feed conversion ratio were significantly affected by any concentrations (P < 0.05). The relative weight of the spleen, and lung was decreased by the growth inhibitory concentrations. The bursa of Fabricius, thymus, and kid- ney were decreased in relative weight by concentrations of $16{\mu}/g$ of diet. The relative weight of the liver and heart were unaffected. The hemoglobin (Hb), hematocrit (HCT), and mean corpuscular hemoglobin (MCH) were decreased at concentration of $16{\mu}/g$ of diet. As compared with control chickens, there was no marked change in serum components except uric acid in T-2 treated chickens. All lymphoid tissues retained atrophic and lymphoid cell depletion throughout the three weeks trial.

키워드

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