Journal of Microbiology and Biotechnology

  • 제16권7호
  • /
  • Pages.1026-1031
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  • 2006
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Production of a Fusion Protein Containing the Antigenic Domain 1 of Human Cytomegalovirus Glycoprotein B

  • Sousa Fani (Centro de Investigacao em Ciencias da Saude, Universidade da Beira Interior) ;
  • Ferreira Susana (Centro de Investigacao em Ciencias da Saude, Universidade da Beira Interior) ;
  • Queiroz Joao (Centro de Investigacao em Ciencias da Saude, Universidade da Beira Interior) ;
  • Domingues Fernanda (Centro de Investigacao em Ciencias da Saude, Universidade da Beira Interior)
  • 발행 : 2006.07.01
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초록

The optimization of the production of a fusion protein containing the antigenic domain 1 (AD-1) is of a great importance, considering its use in diagnostic tests. The fusion protein is produced by the fermentation of a recombinant strain of Escherichia coli containing the plasmid Mbg58, which expresses the AD-1 (aa 484-650) of human cytomegalovirus glycoprotein B as a fusion protein together with aa 1-375 of ${\beta}-galactosidase$. An important characteristic of promoters (lac and derivatives) used in recombinant protein production in E. coli is their inducibility. Induction by IPTG is widely used for basic research; however, its use in large-scale production is undesirable because of its high cost and toxicity. In this work, studies using different inducers and carbon sources for the production of a fusion protein containing the AD-l were performed. The results showed that lactose could be used as an inducer in the fermentation process for the production of this protein, and that expression levels could exceed those achieved with IPTG. The use of lactose for protein expression in E. coli should be extremely useful for the inexpensive, large-scale production of heterologous proteins in E. coli. Addition of sucrose to the fermentation medium improved the yield of recombinant protein, whereas addition of fructose or trehalose decreased the yield.

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참고문헌

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