Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Induction of Kanamycin Resistance Gene of Plasmid pUCD615 by Benzoic Acid and Phenols

  • Mitchell Robert J. (National Research Laboratory on Environmental Biotechnology, Gwangju Institute of Science and Technology, (GIST)) ;
  • Hong Han-Na (National Research Laboratory on Environmental Biotechnology, Gwangju Institute of Science and Technology, (GIST)) ;
  • Gu Man-Bock (School of Life Sciences and Biotechnology, Korea University)
  • Published : 2006.07.01
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Abstract

A kan'::luxCDABE fusion strain that was both highly bioluminescent and responsive to benzoic acid was constructed by transforming E. coli strain W3110 with the plasmid pUCDK, which was constructed by digesting and removing the 7-kb KpnI fragment from the promoterless luxCDABE plasmid pUCD615. Experiments using buffered media showed that this induction was dependent on the pH of the media, which influences the degree of benzoic acid protonation, and the expression levels seen are likely due to acidification of the cytoplasm by uncoupling of benzoic acid. Consequently, the sensitivity of this strain for benzoic acid was increased by nearly 20-fold when the pH was shifted from 8.0 to 6.5. Benzoic acid derivatives and several phenolics also resulted in significantly increased bioluminescent signals. Although these compounds are known to damage membranes and induce the heat-shock response within E. coli, bacterial strains harboring mutations in the fadR and rpoH genes, which are responsible for fatty acid biosynthesis during membrane stress and induction of the heat-shock response, respectively, showed that these mutations had no effect on the responses observed.

Keywords

References

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