Journal of Microbiology and Biotechnology

  • 제16권7호
  • /
  • Pages.1163-1168
  • /
  • 2006
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Cloning, Sequencing and Characterization of Acyltransferase Gene Involved in Exopolysaccharide Biosynthesis of Zoogloea ramigera 115SLR

  • Lee Sam-Pin (Department of Food Science and Technology, Keimyung University) ;
  • Troyano Esperanza (Department of Biology, Massachusetts Institute of Technology) ;
  • Lee Jin-Ho (Department of Chemistry, Keimyung University) ;
  • Kim Hyun-Soo (Department of Microbiology, Keimyung University) ;
  • Sinskey Anthony John (Department of Biology, Massachusetts Institute of Technology)
  • 발행 : 2006.07.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The recombinant plasmid pLEX2FP complements the mutation in Zoogloea ramigera 115MM1, and the complemented mutant produces an exopolysaccharide that shows higher affinity for the calcofluor dye than the exopolysaccharide from Z. ramigera 115SLR, resulting in higher fluorescence intensity under UV light. A compositional and structural analysis of the exopolysaccharide from Z. ramigera 115MM1 showed that the different fluorescent properties were due to a lower content of acetyl groups when compared with Z. ramigera 115SLR exopolysaccharide. These results were in agreement with a sequence analysis of the gene carried in the plasmid pLEX2FP, which appeared to encode an O-acyltransferase highly homologous to the 3-O-acyltransferase of Streptomyces mycarofaciens. The gene encoding the acyltransferase from Z. ramigera 115SLR was expressed as a GST-fusion protein with 70,000 daltons in E. coli.

키워드

참고문헌

  1. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2
  2. Dugan, P. R. 1975. Bioflocculation and accumulation of chemicals by floc-forming organisms. Report ERS-660 1275-032, U.S. Environmental Protection Agency, Office of Research and Development, Cincinnati, Ohio
  3. Easson, Jr., D. D. 1987. Isolation of Zoogloea ramigera I-16-M exopolysaccharide biosynthetic genes and evidence for instability within this region. J. Bacteriol. 169: 4518-4524 https://doi.org/10.1128/jb.169.10.4518-4524.1987
  4. Easson, Jr., D. D. 1988. A recombinant DNA approach to the design and synthesis of novel polysaccharides. MIT, Ph D. Thesis
  5. Franzen, L. E. and A. B. Norberg. 1984. Structural investigation of the acidic polysaccharide secreted by Zoogloea ramigera 115. Carbohydr. Res. 128: 111-117 https://doi.org/10.1016/0008-6215(84)85089-2
  6. Friedman, B. A., P. R. Dugan, R. M. Pfister and C. C. Remsen. 1969. Structure of exocellular polymers and their relationship to bacterial flocculation. J. Bacteriol. 98: 1328-1334
  7. Glucksmann, M. A., T. L. Reuber, and G. C. Walker. 1993. Genes needed for the modification, polymerization, export and processing of succinoglycan by Rhizobium meliloti: A model for succinoglycan biosynthesis. J. Bacteriol. 175: 7045-7055 https://doi.org/10.1128/jb.175.21.7045-7055.1993
  8. Haigler, C. H., R. M. Brown Jr., and M. Benziman. 1980. Calcofluor white ST alters the in vivo assembly of cellulose microfibrils. Science 210: 903-906 https://doi.org/10.1126/science.7434003
  9. Hakomori, S. 1964. A rapid premethylation of glycolipid and polysaccharide catalyzed by methylsulfinyl carbanion in dimethyl sulfoxide. J. Biochem. 55: 205-208
  10. Hara, O. and C. R. Hutchinson. 1992. A macrolide 3-O-acyltransferase gene from the midecamycin-producing species Streptomyces mycrofaciens. J. Bacteriol. 174: 5141-5144 https://doi.org/10.1128/jb.174.15.5141-5144.1992
  11. Ikeda, T., H. Shuto, T. Saito, T Fukui, and K. Toita. 1982. An extracellular polysaccharide produced by Zoogloea ramigera 115. Eur. J. Biochem. 123: 437-445 https://doi.org/10.1111/j.1432-1033.1982.tb19787.x
  12. Laemmli, U. K. 1970. Cleavege of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685 https://doi.org/10.1038/227680a0
  13. Lee, S.-P., O.-S. Kwon, and A. J. Sinskey. 1996. Localization of genes involved in exopolysaccharide biosynthesis in Zoogloea ramigera 115SLR. J. Microbiol. Biotechnol. 6: 321-325
  14. Lee, S.-P., T.-R. Kim, and A. J. Sinskey. 2000. Cloning and sequencing of the gene involved in morphological change of Zoogloea ramigera 115SLR. J. Microbiol. Biotechnol. 10: 161-168
  15. Leigh, J. A., E. R. Signer, and G. C. Walker. 1985. Exopolysaccharide-deficient mutants of Rhizobium meliloti that form ineffective nodules. Proc. Natl. Acad. Sci. USA 82: 6231-6235
  16. Leigh, J. A., J. W. Reed, J. F. Hanks, A. M. Hirsch, and G. C. Walker. 1987. Rhizobium meliloti mutants that fail to succinylate their calcofluor-binding exopolysaccharide are defective in nodule invasion. Cell 51: 579-587 https://doi.org/10.1016/0092-8674(87)90127-9
  17. Norberg, A. B. and S. O. Enfors. 1982. Production of extracellular polysaccharide by Zoogloea ramigera. Appl. Environ. Microbiol. 44: 1231-1237
  18. Prehm, P. 1980. Methylation of methyl trifluoromethanesulfonate in trimethyl phosphate. Carbohydr. Res. 78: 372-374 https://doi.org/10.1016/0008-6215(80)90018-X
  19. Sambrook, J. and D. W. Russell. 2001. Molecular Cloning: A Laboratory Manual, pp. 1.35-1.37. 3rd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, U.S.A
  20. Simon, R., U. Priefer, and A. Puhler. 1983. Vector plasm ids for in vivo and in vitro manipulation of Gram-negative bacteria, pp. 99-105. In Puhler, A. (ed). Molecular Genetics of the Bacteria-plant Interaction. Springer Verlag, New York, U.S.A
  21. Smith, D. B. and K. S. Johnson. 1988. Single step purification of polypeptides in Escherichia coli as fusions with glutathione S-transferase. Gene 67: 31-40 https://doi.org/10.1016/0378-1119(88)90005-4
  22. Sutherland, I. W. 1990. Biotechnology of Microbial Expolysaccharides, pp. 7-8. Cambridge University Press, New York, U.S.A
  23. Troyano, E., S.-P. Lee, C.-K. Rha, and A. J. Sinskey. 1996. Presence of acetate and succinate groups in the exopolysaccaride from Z. ramigera 115. Carbohydr. Poly. 31: 35-40 https://doi.org/10.1016/S0144-8617(96)00056-2
  24. Verma, N. K., J. M. Brandt, D. J. Verma, and A. A. Lindberg. 1991. Molecular characterization of the O-acetyl transferase gene of converting bacteriophage SF6 that adds group antigen 6 to Shigella flexneri. Mol. Microbiol. 5: 71-75 https://doi.org/10.1111/j.1365-2958.1991.tb01827.x