DOI QR코드

DOI QR Code

Biological Control of Garlic White Rot Accused by Sclereotium cepivorum and Sclereotium sp. Using Bacillus subtilis 122 and Trichoderma harzianum 23

Bacillus subtilis 122와 Trichoderma harzianum 23에 의한 마늘 흑색썩음균핵병의 생물적 방제

  • Lee Sang-Yeob (Division of Plant Pathology, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Lee Sang-Bum (Research Policy Planning Division, Research Management Bureau, Rural Development Administration) ;
  • Kim Yong-Ki (Division of Plant Pathology, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Hwang Soon-Jin (Green Biotech)
  • 이상엽 (농촌진흥청 농업과학기술원 식물병리과) ;
  • 이상범 (농촌진흥청 연구개발국 연구정책과) ;
  • 김용기 (농촌진흥청 농업과학기술원 식물병리과) ;
  • 황순진 ((주)그린바이오텍)
  • Published : 2006.08.01

Abstract

Trichoderma harzianum 23 WP and Bacillus subtilis 122 WP were formulated as antagonists of Sclerotium cepivorum and Sclerotium sp. of garlic white rot. In the field test, applications of Trichoderma harzianum WP and Bacillus subtilis WP reduced garlic white rot by Sclerotium cepivrum from 10.9% in the control to 4.1% and 6.2%, respectively at Taean. Also at Seosan, applications of Trichoderma harzianum 23 WP and Bacillus subtilis 122 WP reduced garlic white rot by Sclerotium sp. from 17.8% in the control to 1.2% and 2.6%, respectively. Treatment of Trichoderma harzianum 23 WP and Bacillus subtilis 122 WP increased garlic yield in two area. Therefore, Trichoderma harzianum 23 WP and Bacillus subtilis 122 WP have shown potential as biofungicides of garlic white rot in the two different pathogens.

마늘 흑색썩음균핵병균 (Sclerotium cepivrum, Sclerotium sp.)에 대하여 생물적 방제제 Trichoderma harzianum 23 WP와 Bacillus subtilis 122 WP를 제제화하였다. 2003 년에 포장시험결과에서 태안지역은 남도마늘의 흑색썩음균핵 병균 (Sclerotium cepivrum)에 Trichoderma harzianum 23 WP 와 Bacillus subtilis 122 WP를 습분의 처리한 결과는 무처리 10.9% 비하여 4.1% 와 6.2%로 병 발생이 감소하였다. 서산지역은 서산종 마늘의 흑색썩음균핵병균 (Sclerotium sp.)에 Trichoderma harzianum 23 WP 와 Bacillus subtilis 122 WP를 습분의 처리한 결과는 무처리 17.8% 비하여 1.2% 와 2.6%로 병 발생이 감소하였다. 마늘수량은 두 지역에서 무처리에 비하여 증수되었다. 그러므로 Trichoderma harzianum 23 WP 와 Bacillus subtilis 122 WP는 마늘 흑색썩음병에 생물농약으로서 방제가능성을 나타내었다.

Keywords

References

  1. Clarkon, J. P., Payne, T., Mead, A. and Whipps, J. M. 2002. Selection of fungal biological control agentsfo for control of white rot by sclerotial degradation in a UK soil. Plant Pathology. 51: 735-745 https://doi.org/10.1046/j.1365-3059.2002.00787.x
  2. Copping. L. G. 2004. The manual of biocontrol agents. third edition, BCPC. UK. 702 pp
  3. Crowe, F., Parks, R. and Bafus, R. 2002. Fungicidal control of white rot on garlic and onion in Oregon. Proc. of the 7th international workshop on Allium white rot, June 4-8, 2002. Harris Ranch, California
  4. Esler, G. and Coley-Smith, J. R. 1983. Flavor and odor characteristics of species of Allium in relation to their capacity to stimulate germination of sclerotia of Sclerotium cepivorum. Plant pathology 32: 13-22 https://doi.org/10.1111/j.1365-3059.1983.tb01296.x
  5. Harper, G. E., Frmpton, C. M. and Stewart, A. 2002. Factors influencing survival of sclerotia of sclerotium cepivorum in New Zealand soils. N. Z. J. Crop Hort. Sci. 30: 29-35 https://doi.org/10.1080/01140671.2002.9514196
  6. Hovious, M. H. Y. and McDonald, M. R. 2002. Management of Allium white rot (Sclerotium cepivorum) in onions on organic soil with soil-applied dially disulfide and di-N-propyl disulfide. Can. J. Plant Pathol. 24: 281-286 https://doi.org/10.1080/07060660209507010
  7. Kay, S. J. and Stewart, A. 1994. Evaluation of fungal antagonists for control of onion white rot in soil box trials. Plant Path. 43: 371-377 https://doi.org/10.1111/j.1365-3059.1994.tb02698.x
  8. Kim, Y.-K., Kwon, M.-K., Shim, H.-S., Yeh, W.-H., Kim, T.-S., Cho, W.-D. and Kim, C.-H. 2004. A new method for sclerotial isolation of two species of sclerotium from infested soils. Plant Pathol. J. 20: 240-243 https://doi.org/10.5423/PPJ.2004.20.4.240
  9. McLean, K. L. and Stewart, A. 2000. Application stratgies for control of onion white rot by fungal antagonists. N.Z.J. Crop Hort. Sci. 28: 115-122 https://doi.org/10.1080/01140671.2000.9514131
  10. McLean, K. L., Hunt, J. and Stewart, A. 2001. Compatibility of the biocontrol agent Trichoderma harzianum C52 with selected fungicides. N.Z. Plant Prot. 54: 84-88
  11. Michael Davis, R. 2002. Use of a natural product to stimulate sclerotial germination of Sclerotium cepivorum for the control white rot of onions and garlic. Pest Management Grants Final Report: 1-9
  12. Pinto, C. M. F., Maffia, L. A., Berger, R. D., Mizubutti, E. S. G and Casali, V. W. D. 1998. Progress of white rot on garlic cultivars planted at different times. Plant Dis. 82: 1142-1146 https://doi.org/10.1094/PDIS.1998.82.10.1142
  13. Sandys, W., C., Whipps, J. M., Gerlagh, M. and Kruse, M. 1993. World distribution of the sclerotial mycoparasite Coniothyrium minitans. Mycol. Res. 97: 1175-1178 https://doi.org/10.1016/S0953-7562(09)81280-X

Cited by

  1. Biological Control of White Rot in Garlic Using Burkholderia pyrrocinia CAB08106-4 vol.19, pp.1, 2013, https://doi.org/10.5423/RPD.2013.19.1.021
  2. Antagonistic Activities of Bacillus spp. Strains Isolated from Tidal Flat Sediment Towards Anthracnose Pathogens Colletotrichum acutatum and C. gloeosporioides in South Korea vol.31, pp.2, 2015, https://doi.org/10.5423/PPJ.OA.03.2015.0036
  3. Antifungal Activity of Benzoic Acid from Bacillus subtilis GDYA-1 against Fungal Phytopathogens vol.18, pp.2, 2012, https://doi.org/10.5423/RPD.2012.18.2.109
  4. Control Activities of Fungicides Against Garlic White Rot Caused by Sclerotium cepivorum vol.19, pp.1, 2015, https://doi.org/10.7585/kjps.2015.19.1.64