Nutritional Sciences

The Korean Nutrition Society (한국영양학회)
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Antioxidative and Anti-aging Effects of Sancho (Zanthoxylum schinifolium) Extract in Rats Fed High Fat Diet

  • Jang Mi-Jin (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Woo Mi-Hee (Department of pharmacy, Catholic University of Daegu) ;
  • Rhee Soon-Jae (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Cho Sung-Hee (Department of Food Science and Nutrition, Catholic University of Daegu)
  • Published : 2006.08.01
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Abstract

This study was performed to investigate antioxidative and anti-aging action of extracts from Sancho (Zanthoxylum schinifolium) leaves. Two extracts were obtained by 80% methanol extraction followed by subsequent fractionation with methylene chloride (MC) and n-butanol (B) and fed at one or three levels to rats on normal level (5%) of fat (control) and high fat(20%) in diets. Male Sprague-Dawley rats weighing about 100 g were divided into ten groups such as control diet group(C), control diet+0.50%B group (CB), control diet+0.50%MC group (CMC), high-fat diet group (HF), high-fat diet+0.25%B group (HBL), high-fat diet+0.50%B group (HBM), high-fat diet+0.75%B group (HBH), high-fat diet+0.25%MC group (HMCL), high-fat diet+0.50%MC group (HMCM) and high-fat diet+ 0.75%MC group (HMCH) and fed each diet for four weeks. The effects of the extracts on antioxidant enzyme activities and indices of lipid peroxidation and aging were seen only in high fat diet groups. Hepatic superoxide dismutase and aryleaterase activities were not changed by Sancho extracts. But glutathione peroxidase, catalase and paraxonase activities were significantly restored by both MC and B at the level of 0.75% lipid peroxide which was increased by high fat diet was significantly reduced by B and MC at the level of 0.25% and over. Lipofuscin fluorescence and cabonyl value were increased by high fat diet were reduced by B and MC at the level of 0.5% and over. It is concluded that the Sancho extracts can be utilized as functional ingredients of health foods for reducing oxidative stress.

Keywords

References

  1. Choi HM. NUTRITION & HEALTH. Life Science Publication Co., 2002
  2. Shin DH. Antioxidation substances in mulberry leaf. J Korean Oil Chemists Soc 16(3):27-31, 1998
  3. Kodama T, Ishida H, Kokubo T, Yamakawa T, Noguchi H. Glucosylation of quercetin by a cell suspension culture of vitis species. Agric Biol Chem 54:3238-3288, 1990
  4. Chae JY, Lee JY, Hoang IS, Whangbo D, Choi PW, Lee WC, Kim JW, Kim SY, Choi SW, Rhee SJ. Analysis of functional components of leaf of different mulberry cultivars. J Korean Soc Food Sci Nutr 32:15-21, 2003 https://doi.org/10.3746/jkfn.2003.32.1.015
  5. Frielovich I. The biology of oxygen radicals: The superoxide radical as an agent of oxygen toxicity: Superoxide dismutase provides an important defense. Sci 201:875-880, 1978 https://doi.org/10.1126/science.210504
  6. Naitoh K. Studies on the micro constituent in mulberry leaves part 2. Isolation of rutin and quercetin from mulberry leaves. Nippon Nogei Kagaku Kaishi 42:422-425, 1968
  7. Shin KH, Young HS, Lee TW, Choi JS. Studies on the chemical component and antioxidative effects of solanum lyratum. Kor J Pharmacogn 26:130-138, 1995
  8. Nakayama T. Suppression of hydroperoxide induced cytotoxicity by polyphenol. University of Shizuoka Japan 7:1991-1993, 1994
  9. Chi YS, Jong HG, Son KH, Chang HW, Kang SS, Kim HP. Effects of naturally occurring prenylated flavonoids on enzymes metabolizing arachidonic acid: cylcooxygenases and lipoxygenases. Biochemical pharmacology 62: 1185-1191, 2001 https://doi.org/10.1016/S0006-2952(01)00773-0
  10. Kwak CS, Lee SH. Carboxylesterase, arylesterase and cholinesterase activities in cholestatic rat liver induced by common bile duct ligation. Korean Biodchem J 25(3):251-261, 1992
  11. Park KY, Kang CS, Lee YS, Lee YH, Lee YS. Tocotrienol and tocopherol content in various plant seeds. Korean J Crop Sci 49:207-210, 2004
  12. Cha WS, Shin HR, Park JH, Oh SL, Lee WY, Chun SS, Chao TW, Cho YJ, Antioxidant activity of phenol compounds from Mulberry fruits. Korean J Food Preservation 11:383-387, 2004
  13. Mun SI, Ryu HS, Lee HJ, Choi JS. Further screening for antioxidant activity of vegetable plants and its active principles from Zanthoxylum schinifolium. J Korean Soc Food Nutr 23:466-471, 1994
  14. Jang MJ, Woo MH, Kim YH, Jun DY, Rhee SJ. Effects of antioxidative, DPPH radical scavenging activity and antithrombogenic by the extract of sancho (Zanthoxylum schinifolium). J Korean Nutr 38:386-394, 2006
  15. Lee MS, Chung MS. Analysis of volatile flavor components from Zanthoxylum schinifoliumand sensory evaluation as natural spice. Korean J Soc Food Sci 16:216-220, 2000
  16. Lim SJ, Han HK, Ko JH. Effects of edible and medicinal plants intake on blood glucose, glycogen and protein levels in streptozotocin induced diabetic rats. J Korean Nutr 36:981-989, 2003
  17. Reeves PG, Nielsen FH, Fahey Jr GC. AIN-93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition ad hoc writing committee on the freformulation of the AIN-76A dodent diet. J Nutr 123:1939-1951, 1993 https://doi.org/10.1093/jn/123.11.1939
  18. Marklund S, Marklund G. Involvement of the superoxide anion radical in the antioxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469-474, 1974 https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  19. Richard AL, Raymond FB. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem and BiopHysical Research Communications 71(4):952-958, 1976 https://doi.org/10.1016/0006-291X(76)90747-6
  20. Aebi H, Wyss SR, Scherz B, Skvaril F. Heterogeneity of erythrocyte catalase II. Isolation and Characterization of normal and variant erythrocyte catalase and their subunits. Eur J Biochem 48:137-145, 1974 https://doi.org/10.1111/j.1432-1033.1974.tb03751.x
  21. Mackness MI, Harty D, Bhatnagar D, Winocour PH, Arrol S, Ishola M, Durrington PN. Serum paraoxonase activity in familial hypercholesterolemia and insulin-dependent diabetes mellitus. Atherosclerosis 86:193-199, 1991 https://doi.org/10.1016/0021-9150(91)90215-O
  22. La Du BN, Exkerson HW. The polymorpHic paraoxonase/arylesterase isozymes of human serum. Fed Proc 43:2338-2341, 1992
  23. Satoh K. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica Chemica Acta 90:37-43, 1978 https://doi.org/10.1016/0009-8981(78)90081-5
  24. Fletcher BL, Dillard CJ, Tappel AL. Measurement of fluorescent lipid peroxidation products in biological systems and tissues. Analytical Biochemistry 52:1-9, 1973 https://doi.org/10.1016/0003-2697(73)90327-8
  25. Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn B, Shaltiel S, Stadtman ER. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol 186:464-478, 1990 https://doi.org/10.1016/0076-6879(90)86141-H
  26. Oliver HL, Nira JR, Lewis F, Rose JR. Protein measurement with the folin phenol reagent. J Biol Chem 193:265-275, 1951
  27. Sreel RGD, Torrie JH. Principles and procedures of satistics. McGrow Hill New York, USA, 1990
  28. Mun SI, Ryu HS, Choi JS. Inhibition effects of Zanthoxylum schinifolium and its active principle on lipid peroxidation and liver damage in carbon tetrachloride-treated mice. J Korean Soc Food Nutr 26:943-951, 1997
  29. Mun SI. Effects of Zanthoxylum schinifolium and its active principle on serum lipid levels in carbon tetrachloride-treated mice. Korean J Food & Nutr 13:249-254, 2000
  30. Halliwell B, Gutteridge MC. Free radicals inbiology and medicine. pp.166-170, Oxford University Press Oxford, 1985
  31. Kim MJ, Cho SY, Jang JY, Park JY, Park EM, Lee MK, Kim DJ. Effect of water extract of green tea, persimmon leaf and safflower seed on heme synthesis and erythrocyte antioxidant enzyme activities in lead-administered rats. J Korean Soc Food Sci Nutr 32:191-196, 2003 https://doi.org/10.3746/jkfn.2003.32.2.191
  32. Kim JH, Kwon MJ, Lee SY, Ryu JD, Moon GS, Cheigh HS, Song YO. The effect of kimchi intake on production of free radicals and anti-oxidative enzyme activities in the liver of SAM. J Korean Soc Food Sci Nutr 31:109-116, 2002 https://doi.org/10.3746/jkfn.2002.31.1.109
  33. Kwon RH, Jung MA, Rhee SJ, Choi SW, Cho SH. Antioxidative effects and improvement of lipid metabolism of mulberry fruit, mulberry leaves and silkworm powder with different mixing ratios in streptozotocin-induced diabetic rats. Korean J Nutr 39:91-99, 2006
  34. Kim WS, Kim YS. Paraoxogenase polymorphism as a new genetic marker of atherosclerosis. Korean Society for Lipid and Atherosclerosis 6:81-86, 1996
  35. Griesmacher A, Kindhauser M, Andert SE, Schreiner W, Toma C, Knoebl P, Pietschmann P, Prager R, Schnack C, Schemthaner G. Enhanced serum levels of thiobarbituric-acid-reacive substances in diabetes mellitus. Am J Med 98:469-475. 1995 https://doi.org/10.1016/S0002-9343(99)80347-7
  36. Fang Z. Bioactive constituents from the leaves of Zanthoxylum schinifolium. MS thesis, Dept. of Pharmacy, Daegu catholic University, 2006
  37. Choi JH, Kim DI, Park SH, Kim DW, Lee KG, Yeo JH, Kim JM, Lee YW. Effects of silk fibroin powder on lipofuscin, acetylcholine and Its related enzyme activities in brain of SD rats. Korean J Seric Sci 42:120-125, 2000
  38. Fridovich I. Bioligical effects of the superoxide radical. Arch Biochem Biophys 247:1-15, 1986 https://doi.org/10.1016/0003-9861(86)90526-6