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Biological Control Activity of Two Isolates of Pseudomonas fluorescens against Rice Sheath Blight

  • Choi Gyung-Ja (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Kim Jin-Cheol (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Park Eun-Jin (Division of Applied Biology, College of Natural Resources and Life Science, Dong-A University) ;
  • Choi Yong-Ho (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Jang Kyoung-Soo (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Lim He-Kyoung (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Cho Kwang-Yun (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Lee Seon-Woo (Division of Applied Biology, College of Natural Resources and Life Science, Dong-A University)
  • Published : 2006.09.01

Abstract

Two isolates of mucous bacteria, mc75 and pc78, were isolated from fungal culture plate as culture contaminants with an interesting swarming motility. Both isolates were identified as Pseudomonas fluorescens based on microscopy, biochemical analysis, Biolog test and DNA sequence analysis of the 16S rRNA gene. Both strains have the exactly the same 16S rRNA gene sequences, and yet their biological control activity were not identical each other. In vitro analysis of antagonistic activity of two isolates against several plant pathogenic fungi indicated that both produced diffusible and volatile antifungal compounds of unknown identities. Treatment of the bacterial culture of P. fluorescens pc78 and its culture filtrate exhibited a strong biological control activity against rice sheath blight in vivo among six plant diseases tested. More effective disease control activity was obtained from treatment of bacterial culture than that of culture filtrate. Therefore, in addition to antifungal compound and siderophore production, other traits such as biofilm formation and swarming motility on plant surface may contribute to the biological control activity of P.fluorescens pc78 and mc75.

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