Biological Control Activity of Two Isolates of Pseudomonas fluorescens against Rice Sheath Blight

  • Choi Gyung-Ja (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Kim Jin-Cheol (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Park Eun-Jin (Division of Applied Biology, College of Natural Resources and Life Science, Dong-A University) ;
  • Choi Yong-Ho (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Jang Kyoung-Soo (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Lim He-Kyoung (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Cho Kwang-Yun (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
  • Lee Seon-Woo (Division of Applied Biology, College of Natural Resources and Life Science, Dong-A University)
  • Published : 2006.09.01


Two isolates of mucous bacteria, mc75 and pc78, were isolated from fungal culture plate as culture contaminants with an interesting swarming motility. Both isolates were identified as Pseudomonas fluorescens based on microscopy, biochemical analysis, Biolog test and DNA sequence analysis of the 16S rRNA gene. Both strains have the exactly the same 16S rRNA gene sequences, and yet their biological control activity were not identical each other. In vitro analysis of antagonistic activity of two isolates against several plant pathogenic fungi indicated that both produced diffusible and volatile antifungal compounds of unknown identities. Treatment of the bacterial culture of P. fluorescens pc78 and its culture filtrate exhibited a strong biological control activity against rice sheath blight in vivo among six plant diseases tested. More effective disease control activity was obtained from treatment of bacterial culture than that of culture filtrate. Therefore, in addition to antifungal compound and siderophore production, other traits such as biofilm formation and swarming motility on plant surface may contribute to the biological control activity of P.fluorescens pc78 and mc75.


  1. Caiazza, N. C., Shanks, M. Q. and O'Toole, G. A. 2005. Rhamnolipids modulate swarming motility patterns of Pseudomonas aeruginosa. J. Bacteriol. 187:7351-7361
  2. Cook, R. J. 1993. Making greater use of introduced microorganisms for biological control of plant pathogens. Ann. Rev. Phytopathol. 31 :53-80
  3. DowIing, D. N. and O'Gara, F. 1994. Metabolites of Pseudomonas involved in the biocontrol of plant disease. Trends Biotechnol. 12:133-141
  4. Edwards, U., Rogall, T., Blocker, H., Emde, M. and Bottger, E. C. 1989. Isolation and direct complete determination of entire genes. Nucleic Acids Res. 17:7843-7853
  5. Ferrin , D. M. and Rhode, R. G 1992. In vivo expression of resistance to metalaxyl by a nursery isolate of Phytophthora parasitica from Cathamathus rose us. Plant Dis. 76:82-84
  6. Gerhardt, P., Murray, R. G. E., Costilow, R. N., Nester, E. W., Wood, W. A., Kreig, N. R. and Phillips, G. B. 1981. Manual of methods for general bacteriology, American Society for Microbiology, Washington DC, USA, 524 pp
  7. Haas, D. and Keel, C. 2003. Regulation of antibiotic production in root-colonizing Pseudomonas spp. and relevance for biological control of plant disease. Ann. Rev. Phytopathol. 41:117-153
  8. Handelsman, J. and Parke, J. L. 1989. Mechanisms in biocontrol of soilborne plant pathogens, In: Plant-microbe interactions, Vol. 3, ed. by T. Kosuge and E. W. Nebster, pp. 27-61.McGraw-Hill Publishing Co., New York
  9. Handelsman, J. and Stabb, E. V. 1996. Biocontrol of soilborne plant pathogens. Plant Cell 8:1855-1869
  10. Kay, E., Dubuis, C. and Haas, D. 2005. Three small RNAs jointly ensure secondary metabolism and biocontrol in Pseudomonas fluorescens SHA0. Proc. Natl. Acad. Sci. USA 102:17136-17141
  11. Keane, P. J., Kerr, A. and New, P. B. 1970. Crown gall of stone fruit. II. Identification and nomenclature of Agrobacterium isolates. Aust. J. BioI. Sci. 23:585-595
  12. Kim, J-C., Choi, G. J., Lee, S-W., Kim, J-S., Chung, K. Y. and Cho, K. Y. 2004. Screening extracts of Achyranthes japonica and Rumex crispus for activity against various plant pathogenic fungi and control of powdery mildew. Pest Manag. Sci. 60:803-808
  13. Kremer, R. J. and Soussi, T. 2001.Cyanide production by rhizobacteria and potential for suppression of weed seedling growth. Curr. Microbiol. 43:182-186
  14. Lee, S-W. and Cooksey, D. A. 2000. Genes expressed in Pseudomonas putida during colonization of a plant pathogenic fungus. Appl. Environ. Microbiol. 66:2764-2772
  15. Martinez-Granero, F., Rivilla, R. and Martin, M. 2006. Rhziosphere selection of highly motile phenotypic variants of Pseudomonas fluorescens with enhanced competitive colonization ability. Appl. Environ. Microbiol. 72:3429-3434
  16. O'Sullivan, D. J. and O'Gara, F. 1992. Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens. Microbiol. Rev. 56:662-676
  17. Weller, D. M. 1988. Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Ann. Rev. Phytopathol. 26:379-407
  18. Weller, D. M., Howie, W. J. and Cook, R. J. 1988. Relationship between in vitro inhibition of Gauemannomyces graminis var tritici and suppression of take-all of wheat by fluorescent pseudomonads. Phytopathology 78:1094-1100
  19. Yang, C.-H., Menge, J. A. and Cooksey, D. A. 1994. Mutations affecting hyphal colonization and pyoverdine production in pseudomonads antagonistic toward Phytophthora parasitica. Appl. Environ. Microbiol. 60:473-481
  20. Yourman, L. F. and Jeffers, S. N. 1999. Resistance to Benzimidazole and Dicarboximide fungicides in greenhouse isolates of Botrytis cinerea. Plant Dis. 83:569-575

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