Quality Characteristics of White Bread Added with Chlorella powder

클로렐라를 첨가한 식빵의 품질특성

  • Jeong Chang-Ho (Division of Applied Life Sciences, Graduate School, Institute of Agricultural & Sciences, Gyeongsang National University) ;
  • Cho Hyun-Jae (Division of Applied Life Sciences, Graduate School, Institute of Agricultural & Sciences, Gyeongsang National University) ;
  • Shim Ki-Hwan (Division of Applied Life Sciences, Graduate School, Institute of Agricultural & Sciences, Gyeongsang National University)
  • 정창호 (경상대학교 대학원 응용생명과학부 농업생명과학연구원) ;
  • 조현제 (경상대학교 대학원 응용생명과학부 농업생명과학연구원) ;
  • 심기환 (경상대학교 대학원 응용생명과학부 농업생명과학연구원)
  • Published : 2006.08.01

Abstract

The chemical components of chlorella powder and quality characteristics (texture, color and sensory evaluation etc.) of white bread added with chlorella powder in order to use as a new functional food material of chlorella powder were examined. The proximate composition was composed of crude protein 61.45%, crude fiber 13.47%, nitrogen free extract 13.15%, ash 6.35%, moisture 3.27% and crude fat 2.31%, respectively. The component of major minerals were P (1,478.94 mg%), K (250.54 mg%), Ca (147.24 mg%) and Na (107.26 mg%) and free sugar was composed of maltose 0.12%, glucose 0.09% and fructose 0.08%. The major amino acids of chlorella powder were glutamic acid(4,009.20 mg%), aspartic acid(3,017.72 mg%), proline(3,055.24 mg%), methionine(3,001.52 mg%) and histidine(2,951.06 mg%). The major fatty acids in chlorella powder were composed of linoleic acid and the amounts of those showed 34.27%. The volume tended to increase according to the addition of chlorella powder. Substituting chlorella powder with flour also resulted in decreased lightness, redness and yellowness of the white bread crust The color of white bread crust became darker as the amount of chlorella powder increased. The texture characteristics of white bread containing chlorella powder was no significant difference. The result of sensory evaluation by QDA (quantitative descriptive analysis) showed that overall acceptability of white broads containing 0.2% chlorella powder were higher than that of control without chlorella powder.

클로렐라를 새로운 기능성 식품의 재료로 사용하기 위하여 클로렐라 분말의 화학성분 분석 및 클로렐라 분말을 첨가한 식빵을 제조하여 품질특성을 조사하였다. 클로렐라의 화학성분을 분석한 결과 단백질이 61.45%로 가장 많은 함량을 나타내었으며, 조섬유, 가용성 무질소물 및 회분순으로 나타났다. 무기성분을 분석한 결과 주된 무기성분은 P, K Ca 및 Na로 나타났으며, 그 중 P이 1,478.94 mg%로 가장 많은 함량을 나타났다. 유리당을 분석한 결과 클로렐라에는 sucrose, glucose, fructose 및 maltose 등 총 4종류의 유리당이 함유되어 있었으며, 그 중 maltose가 0.12%로 가장 높게 나타났다. 아미노산을 분석한 결과 총 17종의 아미노산이 분리, 동정되었으며, 총 아미노산은 37,425.11 mg%로 나타났고, 그 중 aspartic acid, glutamic acid, proline, methionine 및 histidine이 주요 아미노산으로 나타났으며, glutamic acid가 4,009.20 mg%로 가장 높게 나타났다. 지방산 조성을 분석한 결과 불포화지방산이 전체지방산의 68.87%를 차지하였으며, 그 중 linoleic acid가 34.27%로 가장 높게 나타났다. 클로렐라를 첨가하여 제조한 반죽을 발효시간 경과에 따른 부피 변화를 측정한 결과 발효시간이 경과함에 따라 반죽의 부피가 증가하는 경향을 나타내었으나 대조구와 비교하여 클로렐라 분말의 첨가량이 증가함에 따라 부피가 증가하는 경향을 나타내었다. 식빵의 crust와 crumb의 색도는 클로렐라의 첨가량이 증가함에 따라 밝기를 나타내는 L값과 적색도를 나타내는 a값은 crust와 crumb 모두 감소하는 경향을 나타내었으며, 황색도를 나타내는 b값의 경우 crust는 감소하였고, crumb는 증가하는 것으로 나타났다. 클로렐라 분말을 첨가한 식빵의 조직감은 유의적인 차이를 보이지 않았으며, 관능검사를 실시한 결과 전체적인 기호도에서 클로렐라 분말을 0.2% 첨가한 시료에서 가장 좋은 기호도를 나타내었다.

Keywords

References

  1. Kang, M.S., Sim, S.J. and Chae, H.J. (2004) Chlorella as a functional biomaterial. Korean J. Biotechnol. Bioeng., 19, 1-11
  2. 村上千秋,高橋次郞, 新保園弘,丸山武紀. (1997) クロレラ の脂質成分巧脂肪酸組成.日本油化學曾誌. 46, 423-427
  3. 한재갑, 강기권, 김진국, 김상환. (2002) 클로렐라 추출물 현황 및 전망. 식품과학과 산업, 35, 64-69
  4. 이유경, 이홍금. (2002) 조류의 산업적 이용. 생물산업, 15, 19-24
  5. Umezawa, I., Komiyama, K., Shinbukawa, N., Mori, M. and Kojima, Y. (1982) An acidic polysaccharide, chlon A, from Chlorella pyrenoidosa. Chemotherapy, 30, 1041-1045
  6. Kobayashi, S. (1978) Influence of chlorella extract on reticuloendotherelial of rats. Health and industry Newsletter, Agriculture Chemical Convention
  7. Morita, K. and Matsueda, T. (1999) Chlorella accelerates dioxin excretion in rats. J. Nutr., 129, 1731-1736 https://doi.org/10.1093/jn/129.9.1731
  8. Tanaka, K., Konishi, F. and Himeno, K. (1984) Augmentation of antitumor resistance by a strain of unicellular green algae Chlorella vulgaris. Cancer Immunol. Immunother, 17, 90-94 https://doi.org/10.1007/BF00200042
  9. Lin, J.K. (1981) Effect of chIorella on serum cholesterol of rats. Taiwan Medical Science Journal
  10. 백승화. (1989) ChIorella ellipsoidea 첨가식이가 흰쥐의 혈청지질 성분 및 효소활성도에 미치는 효과, 명지대학교 박사학위논문
  11. Wang, C.J., Shiow, S.J. and Lin, J.K. (1981) Effect of chIorella an the level of serum cholesterol in rats. J. Formosan Med. Assoc., 80, 929-933
  12. Shimizu, M., Yamada, N., Hisada, M., Suzuki, J. and Inata, I. (1985) Effect of chlorella on human pulse wave velocity. Kanazawa Medical University, Dept. of Serology
  13. Want, L.F., Lin, J.K. and Tung, Y.C. (1980) Effect of chlorella on the levels of glycogen, triglyceride and cholesterol in ethionine treated rats. J. Formosan Medical Assoc., 79, 1-10
  14. Young, R.W. and Beregi, J.S. (1980) Use of chIoreophyllin in the care of Geriatric patients. J. Am. Geriatrics Soc., 28, 46-47 https://doi.org/10.1111/j.1532-5415.1980.tb00124.x
  15. Nagano, T., Watanabe, Y., Homma, T., Suketa, Y. and Yamamoto, T. (1978) Absorption and excretion of cadmimn by the rat administered cadmimn containing chIorella. Eisei Kagaku, 24, 182-186 https://doi.org/10.1248/jhs1956.24.182
  16. Hagino, Y. (1975) Effect of chlorella on urinary cadmimn excretion in ' Itai-itai '. J. Hyg., 30, 77-79
  17. Nakamura, M. (1978) Promotion of reticuloendothelial function by chIorella components. Health and industry Newsletter, Agricultural Chemical Convention
  18. 菅野敏博, 新保園弘,政田正弘,田村五郎. (1996) クロレラ 熱水抽出物由來の酵母に對する生育促進物質. 生物工學曾誌, 74, 159-162
  19. 김동호, (1998) 클로렐라, 식품저널, 1, 64-69
  20. Kang, M.S., Sim, S.J. and Chae, H.J. (2004) Partial purification of anticancer and anticoagulant proteins from chIorella hydrolysate. Food Engineering Progress, 8, 98-104
  21. Kim, Y.H., Hwang, Y.K., Hwang, J.M., Seoung, H.K. and Kim, D.U. (2003) Effects of chIorella dietary supplementation on bone biochemical markers of tmnover in postmenopausal women. J. Biomed. Lab. Sci., 9, 9-13
  22. Park, M.K., Lee, J.M., Park, C.H. and In, M.J. (2002) Quality characteristics of Sulgidduk containing chIorella powder. J. Korean Soc. Food Sci. Nutr., 31, 225-229 https://doi.org/10.3746/jkfn.2002.31.2.225
  23. Kim, S.S., Park, M.K., Oh, N.S., Kim, D.C., Han, M.S. and In, M.J. (2003) Studies on quality characteristics and shelf-life of chIorella soybean curd (Tofu). J. Korean Soc. Agric. Chem. BiotechnoI., 46, 12-15
  24. Park, S.l. and Cho, E.J. (2004) Quality characteristics of noodle added with chIorella extract. Korean J. Food & Nutr., 17, 120-127
  25. Kim, J.S. (2004) Preparation of chIorella drinks and its quality characteristics. Korean J. Food & Nutr., 17, 382-387
  26. AOAC. (1990) Official methods of Analysis. 15 th ed. Association of Official Analytical Chemists, Washington D.C
  27. Jeong, C.H., Bae, Y.I., Lee, H.J. and Shim, K.H. (2003) Chemical components of propolis and its ethanolic extracts. J. Korean Soc. Food Sci. Nutr., 32, 501-505 https://doi.org/10.3746/jkfn.2003.32.4.501
  28. Choi, J.H., Jang, J.G., Park, K.D., Park, M.H. and Oh, S.K. (1981) High performance liquid chromatographic determination of free sugars in ginseng and its products. Korean J Food Sci. TechnoI., 13, 107-113
  29. Jeong, C.H. and Shim, K.H. (2004) Quality characteristics of sponge cakes with addition of Pleurotus eryngii mushroom powders. J. Korean Soc. Food Sci. Nutr., 33, 716-722 https://doi.org/10.3746/jkfn.2004.33.4.716
  30. Metcalf, L.D., Schmits, A.A. and Pelka, J.R. (1966) Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Anal Chem., 38, 514-515 https://doi.org/10.1021/ac60235a044
  31. Lee, S.Y. and Kim, C.S. (2001) Effects of added yam powders on the quality characteristics of yeast leavened pan breads made from imported wheat flour and Korean wheat flour. J. Korean Soc. Food Sci. Nutr., 30, 56-63
  32. Chun, S.S., Park, J.R., Cho, Y.S., Kim, M.Y., Kim, R.Y. and Kim, K.O. (2001) Effect of onion powder addition on the quality of white bread. Korean J. Food & Nutr., 14, 346-354
  33. Lee, S.H., Park, H.C. and Her E.S. (1998) Statistics and Date Analysis Method. Hyoil press, Seoul. p. 253-296
  34. Kim, Y.H. (1999) The effect on bioactivities of Chlorella, Food Ind., 10, 122-128
  35. Sung, Y.M., Cho, J.R., Oh, N.S., Kim, D.C. and In, M.J. (2005) Preparation and quality characteristics of curd yogurt added with chIorella. J. Korean Soc. Appl. BioI. Chem., 48, 60-64
  36. Park, S.I. (2003) Effect of chIorella growth factor on quality of bread. Korean J. Food Culture, 18, 356-364