Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Genotoxicological Safety of High-Dose Gamma-Irradiated Cereal Powders

고선량 감마선조사 곡류 분말의 유전독성학적 안전성평가

  • Han Sag-Myung (Department of Food Science and Nutrition & The Korean Institute of Nutrition, Hallym University) ;
  • Kim Hye-Mi (Department of Food Science and Nutrition & The Korean Institute of Nutrition, Hallym University) ;
  • Jeung Seung-Kyoung (Department of Food Science and Nutrition & The Korean Institute of Nutrition, Hallym University) ;
  • Lee Ju-Woon (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Byun Myung-Woo (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Kang Il-Jun (Department of Food Science and Nutrition & The Korean Institute of Nutrition, Hallym University)
  • 한삭명 (한림대학교 식품영양학과, 한국영양연구소) ;
  • 김혜미 (한림대학교 식품영양학과, 한국영양연구소) ;
  • 정승경 (한림대학교 식품영양학과, 한국영양연구소) ;
  • 이주운 (한국원자력연구소) ;
  • 변명우 (한국원자력연구소) ;
  • 강일준 (한림대학교 식품영양학과, 한국영양연구소)
  • Published : 2006.08.01
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Abstract

Gamma irradiation at 30 kGy was applied to cereal powders to evaluate their possible genotoxicity. The genotoxicity of 30 kGy-irradiated cereal powders was evaluated by Salmonella typhimurium reversion assay, chromosomal aberration test and in vivo micronucleus assay. The result were negative in the bacterial reversion assay with S. typhimurium TA98, IA100, TA1535 and TA1537. No mutagenicity was detected in the assay with and without metabolic activation. In chromosomal aberration tests with CHL cells and in vivo mouse micronucleus assay, no significant difference in the incidences of chromosomal aberration and micronuclei was observed between non-irradiated and 30 kGy-irradiated cereal powders. These result indicate that cereal powders irradiated at 30 kGy did not show any genotoxic effect under these experimental conditions.

곡류분말의 위생화 및 물리적 특성 개선을 위해 감마선 조사기술의 이용 가능성이 높아짐에 따라 이들의 안전성을 확보할 목적으로 30 kGy의 고선량 감마선조사 곡류분말의 유전독성학적 안전성 평가를 실시하였다. 감마선 조사 및 비조사 곡류분말의 S. typhimurium TA 98, TA100, TA1535 및 TA1537에 대한복귀변이 집락수를 조사한 결과, 대사활성계 도입 및 부재시 모두 시험적용 농도인 0.625-10 mg/plate의 범위에서 복귀변이 집락수의 농도 의존적인 증가 혹은 감소를 보이지 않아 감마선 조사 곡류분말(30 kGy)은 돌연변이원성이 없는 것으로 나타났다. 또한, 설치류 망상적혈구를 이용하여 감마선조사 곡류분말의 소핵 형성 시험을 수행한 결과, 30 kGy 감마선 조사 곡류분말은 시험적용 용량인 625-5,000 mg/kg의 범위에서 소핵을 가진 망상 적혈구의 출현율이 음성대조군과 유의한 차이를 나타내지 않아 소핵을 유발하지 않음을 확인하였다. 포유류 배양세포를 이용한 염색체 이상시험에서도 30 kGy 감마선 조사곡류분말은 시험적용 용량에서 염색체이상 유발능이 5% 미만이었다.

Keywords

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