Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Determination of Fructooligosaccharides and Raffinose in Infant Formula by High Performance Liquid Chromatography with Evaporative Light Scattering Detector

HPLC-ELSC를 이용한 조제분유 중 fructooligosaccharides 및 raffinose 분석

  • Shin, Man-Sub (National Veterinary Research and Quarantine Service) ;
  • Park, Jae-Woo (National Veterinary Research and Quarantine Service) ;
  • Cho, Mi-Ran (National Veterinary Research and Quarantine Service) ;
  • Song, Sung-Ok (National Veterinary Research and Quarantine Service) ;
  • Kim, Chun-Sun (National Veterinary Research and Quarantine Service) ;
  • Choi, Chun-Bae (National Veterinary Research and Quarantine Service) ;
  • Lee, Seoung-Won (National Veterinary Research and Quarantine Service) ;
  • Lee, Ki-Woong (Research and Development Center, Namyang Dairy Products Corporation) ;
  • Chang, Chi-Hoon (Research and Development Center, Namyang Dairy Products Corporation) ;
  • Kwak, Byung-Man (Research and Development Center, Namyang Dairy Products Corporation)
  • Published : 2006.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

A method was developed for the determination of fructooligosaccharides and raffinose contents in infant formula. The samples were extracted and analyzed by liquid chromatography equipped with carbohydrate column and evaporative light scattering detector. The mobile phase used for the gradient mode was water-acetonitrile, at a flow rate of 1.0mL/min. The method showed a mean recovery of 95-99%, the relative standard deviation obtained in the precision study was 0.774-8.982%, the quantification and detection limits were 25-50mg/L.

본 연구에서는 1-kestose, raffinose, nystose 및 $1^{F}-{\beta}-fructofura-nosylnystose$ 등 4종 올리고당 분석을 위하여 기울기용매가 가능 한 증기화광산란검출기를 사용하여 이동상 조성, 유속 등 최적 조건을 설정하였다. 설정한 분석조건을 이용하여 표준액을 농도별로 희석(50, 100, 200, 400, 800${\mu}g/mL$)하여 검량선을 quadratic curve로 작성한 결과 상관계수는 1-kestose(1.0000), raffinose(0.9999), nystose(1.0000), $1^{F}-{\beta}-fructofuranosylnystose$(1.0000)으로 나타났다. 또한 분석조건의 재현성을 측정하기 위하여 혼합표준액 200${\mu}g/mL$을 5회 주입한 면적 값의 RSD는 1-kestose(1.47), raffinose(8.98), nystose(0.77), $1^{F}-{\beta}-fructofuranosylnystose$(2.02)으로 나타났다. 또한 사용된 시료의 프락토올리고당의 측정된 함량 결정계수에 대한 t-검정결과 모든 표준당에 대해 상관이 유의(p<0.05)함을 알 수 있어 본 HPLC-ELSD방법에 의한 정량이 타당한 것으로 나타났다. 본 방법을 통하여 fructooligosacchrides 및 raffinose분석에 유용한 것으로 사료된다.

Keywords

References

  1. The Korean Nutrition Society. Recommended Dietary Allowances for Koreans. 7th rev. The Korea Nutrition Society, Seoul, Korea. p. 490 (2001)
  2. Korea Food and Drug Administration. Food Standards Codex. Korean Foods Industry Association, Seoul, Korea. pp. 170-180 (2005)
  3. AOAC. Official Methods of Analysis of AOAC Intl. 17th ed. Methods 50.1.01-25. Association of Official Analytical Chemists, Gaithersbrug, Maryland, USA. (2000)
  4. WHO. Technical Report Series, No. 797, Diet, Nutrition, and the Prevention of Chronic Diseases. (1990)
  5. Gibson GR, Roberfroid MB. Dietary modulation of the human colonic microbiota, J. Nutr. 125: 1401-1412 (1995)
  6. Chen HL, Lu YH, Lin J Jr, Ko LY. Effects of fructooligosaccharides on bowel function and indicators of nutritional status in constipated elderly men. Nutr. Res. 20: 1725-1733 (2000) https://doi.org/10.1016/S0271-5317(00)00274-8
  7. Tomomatsu H. Health effects of oligosaccharides. Food Technol. 48: 61-65 (1994)
  8. Umeki K, Kainuma K. Fractionation of maltosaccharides of relatively high degree of polymerization by multiple descending paper chromatography. J. Chromatogr. A. 150: 242-245 (1978) https://doi.org/10.1016/S0021-9673(01)92123-5
  9. Collins FW, Chandorkar KR. Thin-layer chromatography of fructo-oligosaccharides. J. Chromatogr. A. 56: 163-167 (1971) https://doi.org/10.1016/S0021-9673(00)97796-3
  10. Lee JH, Lee HW, Lee HK, Cho DL, Sunwo CS, Park KD, Choi JS, Kim DW, Kim DM. Simple and quantitative analysis method for total carbohydrate concentration in oligosaccharides by using TLC. Korean J. Biotechnol. Bioeng. 19: 269-273 (2004)
  11. Yoo JW, Choo GY, Rho SG, Kim TY, Cho SY, Kim SJ. Adsorption and separation characteristics of maltooligosaccharides. J. Korean Ind. Eng. Chem. 14: 787-792 (2003)
  12. Pontis HG. Separation of fructosans by gel filtration. Anal. Biochem. 23: 331-333 (1968) https://doi.org/10.1016/0003-2697(68)90363-1
  13. Points HG. Observations on the de novo systhesis of fructosans in vivo. Arch. Biochem. Biophys. 116: 416-424 (1966) https://doi.org/10.1016/0003-9861(66)90048-8
  14. Christopher JP. Oligosaccharide intermediates of fructan synthesis in Lolium temulentum. Phytochem. 21: 2461-2465 (1982) https://doi.org/10.1016/0031-9422(82)85241-2
  15. Christopher JP, Micheal AH, David PR. Structural analysis of fructose polymers by gas-liquid chromatography and gel filtration. J. Chromatogr. A. 171: 411-415 (1979) https://doi.org/10.1016/S0021-9673(01)95324-5
  16. Bancal P, Gaudillere JP. Oligofructan separation and quantification by high performance liquid chromatography. Plant Physiol. Bioch. 27: 745-749 (1989)
  17. Scobell HD Brabst KM. Rapid high-resolution separation of oligosaccharides on silver form cation-exchange resins. J. Chromatogr. A. 72: 51-64 (1981) https://doi.org/10.1016/0021-9673(72)80007-4
  18. Kang KJ, Cho JI. Comparison of colorimetry and HPLC method for quantitative analysis of chitooligosaccharide, Korean J. Food Sci. Technol. 32: 788-791 (2000)
  19. Ferreira IMPLVO, Gomes AMP, Ferreira MA. Determination of sugars, and some other compounds in infant forulae, follow-up milks and human milk by HPLC-UV/RI. Carbohyd. Polym. 37: 225-229 (1998) https://doi.org/10.1016/S0144-8617(98)00064-2
  20. Campbell JM, Bauer LL, Fahey GC, Jr., Hogarth AJCL, Wolf BW, Hunter DE. Selected fructooligosaccharide (1-Kestose, Nystose, and $1^{F}-{\beta}$-Fructofuranosylnystose) composition of foods and feeds. J. Agric. Food Chem. 45: 3076-3082 (1997) https://doi.org/10.1021/jf970087g