Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Evaluation of Antimicrobial Activity and Mutagenicity of Ozonized Olive Oil

오존화 올리브유의 항균성 및 변이원성 평가

  • Published : 2006.12.31
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Abstract

Ozonized olive oil was tested for its mutagenic potential in a Salmonella/microsome assay. Additionally, antimicrobial activity was tested against Propionibacterium acnes, Staphylococcus epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa, pathogenic strains related to acne, using the paper disk and agar dilution method. Ozonized olive oil showed antimicrobial activities against all the strains tested, with minimal inhibitory concentrations (MICs) values in a range of 2${\sim}$10 mg/mL. Mutagenicity of ozonized olive oil was evaluated with Salmonells typhimurium TA98, TA100 and Ta1535, with and without addition of S9 mixture. No increase in the number of $his^{+}$ revertants over the negative control (solvent and non-ozonized olive oil) values was observed with TA98 (1,000 ${\mu}g/plate$), TA100 (1,500 ${\mu}g/plate$) and TA1535 (1,500 ${\mu}g/plate$) strains. The results from this study suggested that ozonized olive oil does not show any mutagenic potential.

오존화 올리브유의 여드름 치료용 소재로서의 사용 가능성을 알아보기 위하여 여드름 형성에 관여하는 미생물인 Propionibacterium acnes, Staphylococcus epidermidis, S. aureus와 Pseudomonas aeruginosa에 대한 생육 억제효과를 검토하였고, Ames test로 그의 변이원성 유무를 평가하였다. 항균활성을 paper disk 법으로 평가했을 때 P. acnes, S. epidermidis, S. aureus, P. aeruginosa에 대하여 항균활성을 나타내기 시작한 농도는 각각 4, 1, 0.5, 0,5 mg/mL이었고, 20 mg/mL 농도에서의 생육 저지환의 크기는 각각 7.7, 5.3, 5.6, 5.5 mm이었다. 50% 생육저해활성을 표시하는 $MIC_{50}$은 P. acnes, S. aureus, P. aeruginosa에 대해 2 mg/mL, S. epidermidis에 대해 4 mg/mL로 나타났으며 미생물의 생육이 관찰되지 않는 MIC 농도는 P. acnes, S. epidermidis, P. aeruginosa에 대해 8 mg/mL, S. aureus에 대해 10 mg/mL로 나타났다. 최소저해농도(MICs) 범위는 2${\sim}$10 mg/mL이었다. Salmonella typhimurium TA98, TA100, TA1535 균주에 대하여 오존화 올리브유와 오존화 메칠 올레산은 S9 mix의 첨가 유무에 상관없이 세포독성을 나타내지 않는 농도에서 $his^{+}$ 복귀돌연변이주의 수가 증가하지 않아 변이원성을 나타내지 않았다. 오존화 올리브유는 돌연변이원성을 나타내지 않으면서 여드름 형성 원인균에 대한 생육 억제효과를 나타내어 여드름 치료용 소재로서 사용할 수 있을 것으로 생각된다.

Keywords

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