Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Biological Activities of Oenothera laciniata Extracts (Onagraceae, Myrtales)

애기달맞이꽃(Oenothera laciniata Hill) 추출물의 생리활성 탐색

  • Lee, Jung-A (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute) ;
  • Kim, Ji-Young (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute) ;
  • Yoon, Weon-Jong (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute) ;
  • Oh, Dae-Ju (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute) ;
  • Jung, Yong-Hwan (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute) ;
  • Lee, Wook-Jae (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute) ;
  • Park, Soo-Yeong (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute)
  • 이정아 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 김지영 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 윤원종 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 오대주 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 정용환 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 이욱재 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 박수영 ((재)제주하이테크산업진흥원 제주생물종다양성연구소)
  • Published : 2006.12.31
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Abstract

The biological activities of Oenothera laciniata extracts were measured, including antioxidant activity and cytotoxic effects. O. laciniata is an endemic species of Jeju Island, Korea with a seaside habitat. The concentration of total polyphenolic compounds from ethanol (EtOH), n-hexane, dichloromethane ($CH_2Cl_2$), ethylacetate (EtOAc), butanol (BuOH), and water fractions of O. laciniata was 63.96, 8.49, 28.11, 172.64, 114.56, and 34.91 mg/g, respectively. The EtOAc fraction contained the highest antioxidative activities ($IC_{50}$), measured as follows: 16.19 ${\mu}g/mL$ in DPPH radical scavenging capacity, 220.37 ${\mu}g/mL$ in xanthine oxidase inhibitory activity, 42.07${\mu}g/mL$ in superoxide radical scavenging capacity, and 421.33 ${\mu}g/mL$ in nitric oxide scavenging capacity. The cytotoxicity of O. laciniata extracts was examined through their effect on the growth of HL-60 cells. Incubation of HL-60 cells with the EtOAc fraction resulted in the greatest inhibition of cell growth; high DNA fragmentation and numerous sub-G1 hypodiploid cells were observed in HL-60 cell cultures treated with the EtOAc fraction. These results suggest that the EtOAc fraction of O. laciniata has potent apoptotic and antioxidative activities in vitro.

본 실험에서는 애기달맞이꽃(Oenothera laciniata Hill)를 80% EtOH로 추출하고 추출물을 극성에 따른 용매 분획을 실시하여 분리된 분획의 총 페놀화합물 함량과 DPPH radical 소거, xanthine oxidase 저해활성, superoxide 소거활성 및 nitric oxide 소거활성 및 세포독성 등의 생리 활성을 측정하였다. 본 연구를 통해 애기달맞이꽃은 산소 유리기의 자유기를 소거할 수 있는 우수한 생리 활성물질을 다수 함유하고 있음을 확인 할 수 있었으며 분획 레벨에서의 활성이 대조구 성분 못지않게 우수하게 나타났다. 또한 총 폴리페놀 함량과 세포독성이 가장 높게 나타난 EtOAc 분획물을 농도별로 HL-60세포에 처리한 결과, 세포성장 억제는 농도 의존적으로 나타났으며, 따라서 세포성장 억제효과가 apoptosis 유도에 의한 것이지를 확인하고자, apoptosis 유도에 의하여 나타나는 현상의 하나인 DNA 단편화 현상을 비롯하여 세포 형태학적 변화 및 유동세포분석기를 통한 DNA content을 측정하였다. 그 결과, 애기달맞이꽃 추출물에 의한 세포증식 억제효과는 apoptosis유도에 의해 세포사멸이 일어남을 확인할 수 있었다.

Keywords

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