대한본초학회지 (The Korea Journal of Herbology)

  • 제21권4호
  • /
  • Pages.85-92
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  • 2006
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지

가매보양환오탕(加昧補陽還五湯)의 SH-SY5Y 뇌신경세포에서 산화적 손상에 의한 세포사멸에 대한 보호효과

Protective effects of added Bo-Yang-Hwan-Oh-Tang on $H_2O_2-induced$ neurotoxicity in SH-SY5Y neuronal cells

  • 한형수 (경북대학교 의과대학 생리학교실) ;
  • 박용기 (동국대학교 한의과대학 본초학교실)
  • Han, Hyung-Soo (Department of Physiology, School of Medicine, Kyungpook National University) ;
  • Park, Yong-Ki (Dept. of Herbology, College of Oriental Medicine, Dongguk University)
  • 발행 : 2006.12.30
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초록

Objectives : To evaluate the neuroprotective effects of added Bo-Yang-Hwan-Oh-Tang (BHT), we investigated the neuronal death protection effects to oxidative damages in SH-SY5Y neuronal cells. Methods : To study the cytotoxic effects of BHT on SH-SY5Y cells, the cell viability was determined by MTT assay. To investigate the neuronal death protection of BHT, SH-SY5Y cells were induced oxidative damages by $H_2O_2$ and then assayed the cell viability and DNA fragmentation. We also investigated DPPH free radical scavenging effect of BHT by tube test. Results : In MTT assay, $1000{\mu}g/ml$ of BHT was not showed the cytotoxic effect on SH-SY5Y cells. BHT protected SHSY5Y cells from $H_2O_2-induced $ neuronal cell death in a dose-dependent manner. BHT also protected SH-SY5Y cells from $H_2O_2-induced$ DNA fragmentation. BHT effectively scavenged DPPH free radicals in a dose-dependent manner. Conclusion : These data suggest that BHT may have strong antioxidant effects through the free radical scavenging and neuroprotective effects in human neuronal cells.

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